Gene delivery carrier

ABSTRACT

Disclosed are methods, compositions, reagents, systems, and kits to prepare and utilize poly (β-amino ester) (PBAE) polymers, which are synthesized via Michael addition reactions of diacrylates and amines disclosed herein. Various embodiments utilize lactones and lactone derivatives to generate the diacrylate compounds. The PBAE polymers are shown to be effective biodegradable carriers for the delivery of an agent such as an organic molecule, inorganic molecule, nucleic acid, protein, peptide, polynucleotide, targeting agent, an isotopically labeled chemical compound, vaccine, or an immunological agent.

RELATED APPLICATIONS

This application is a continuation of U.S. application Ser. No.16/179,698, filed Nov. 2, 2018, which claims priority under 35 U.S.C. §119(e) to U.S. Provisional Application Ser. No. 62/581,277, filed onNov. 3, 2017, and to U.S. Provisional Application Ser. No. 62/661,864,filed on Apr. 24, 2018, each of which is incorporated herein byreference in their entirety.

BACKGROUND OF THE INVENTION

Chemical materials have been widely used in biological research andmedicine for the development of delivery systems for a variety ofpayloads including organic molecules, inorganic molecules, nucleicacids, proteins, peptides, polynucleotides, targeting agents,isotopically labeled chemical compounds, vaccines, and immunologicalagents.¹ In particular, chemical delivery systems, such as lipids,polymers, and hydrogels, have helped advance the field of gene delivery,in which efficacious delivery of naked polynucleotides to the site ofinterest is a challenge due to their clearance by the immune system,their inability to cross the cell membrane, and their potentialdegradation in lysosomes.²

Gene delivery is a process by which foreign nucleic acids (DNA and RNA)are transferred to host cells for applications such as genetic researchor gene therapy. Gene delivery methods can be biological (e.g., viral orbacterial vectors), mechanical (e.g., microinjection, electroporation,or biolistics), or chemical (e.g., lipid or nanoparticle carriers).¹ Theideal gene delivery and transfection systems should have hightransfection efficiency, high cell specificity, and low cell toxicity.

Biological gene delivery systems exhibit well-known adverse effects,such as immunogenicity and application limitations, such as difficultiesin handling, difficulties in large-scale production, and limited lengthof the nucleic acid to be delivered.^(3,4) Mechanical gene deliverymethods also present several drawbacks. For example, mechanical methodscause damage to cells, and involve difficulty in large-scalemanipulation, labor-intensive protocols, and/or the necessity of costlyinstruments.⁵ To overcome the drawbacks of biological and mechanicalsystems, a variety of chemical gene delivery vehicles, such as calciumphosphates, lipids, and cationic polymers, including polyamidoaminedendrimers, polyethylenimine (PEI), poly (β-amino esters) (PBAEs), havebeen developed since the late 1960s.⁶⁻⁸

Cationic polymers are among the most common materials that have beenused for gene delivery given their ability to electrostatically bind andcondense nucleic acids to form nanoparticles.

Although many types of cationic polymers have been synthesized andtested as possible chemical delivery systems for gene delivery, such aspolyethyleneimine (PEI), poly(amido-amine) (PAMAM), and poly (β-aminoester) (PBAE) polymers, only CALAA-01, a cyclodextrin containingpolymer, has entered the clinic for siRNA delivery.¹³ High net-positivecharge and inability to undergo degradation under physiologicalconditions or the production of potentially toxic degradation products,which can accumulate in the body at hazardous levels, are the mainreasons limiting clinical applications of known cationic polymers. Ofthe known cationic polymers, PBAEs are particularly useful due to theirhigh transfection efficiency and biodegradability. As such, thereremains a need for new, improved PBAEs for gene delivery.

SUMMARY OF THE INVENTION

Described herein are novel PBAEs derived from polylactone diacrylates.Poly-caprolactone (PCL) and poly-lactic acid (PLA) based polymers areuseful in biomedical applications due to their biodegradability andbiocompatibility.¹⁴ Several PCL and PLA derivatives are already approvedby the FDA for human use. However, existing PCL- and PLA-based polymersare not positively charged, and therefore are unsuitable forapplications related to gene delivery. The use of polylactone-baseddiacrylates in the preparation of PBAEs is expected to produce PBAEswith high transfection efficiency and improved biocompatibility.Polylactone-based diacrylates can be synthesized via a novel two-stepprocedure that consists of (i) a ring opening polymerization of alactone initiated by a mono-acrylate-containing nucleophile, and (ii)acylation of the polylactone mono-acrylate with an acryloylelectrophile. See FIG. 4.

The ring opening polymerization depicted in the first step in FIG. 4 isa so-called “living polymerization”, a technique that allows controlover the number of units of lactone monomers, which in turn allows forcontrol over the polylactone's physical properties, such as molecularweight and lipophilicity.

Methods, compositions, reagents, systems, and kits for the preparationand utilization of polylactone-based PBAEs are also disclosed herein. Incertain embodiments, a polylactone diacrylate is reacted with one ormore amines in a step-growth polymerization to yield acrylate-terminatedPBAEs. The terminal acrylate groups can be reacted with nucleophiles(e.g., amines) to form end-functionalized PBAEs. See FIG. 5.

In one aspect, provided herein is a polymer of Formula (I):

or a pharmaceutically acceptable salt thereof,wherein:

is a single bond or a double bond

A is hydrogen or —XR₃;

each B independently is a diradical of the formula:

each D independently is:

E is hydrogen or —XR₄;

each R, R′, and R″ independently is optionally substituted, cyclic oracyclic aliphatic; optionally substituted, cyclic or acyclicheteroaliphatic; or a combination thereof;

each R_(a) independently is hydrogen or optionally substitutedaliphatic;

R₁ and R₂ independently are selected from hydrogen; optionallysubstituted, cyclic or acyclic aliphatic; optionally substituted, cyclicor acyclic heteroaliphatic; optionally substituted aryl; optionallysubstituted heteroaryl; and a nitrogen protecting group; or R₁ and R₂are combined to form a ring;

R₃ and R₄ independently are selected from hydrogen; optionallysubstituted, cyclic or acyclic aliphatic; optionally substituted, cyclicor acyclic heteroaliphatic; optionally substituted aryl; optionallysubstituted heteroaryl; and a protecting group;

X is O, S, NH, or NR_(X), wherein R_(X) is optionally substituted,cyclic or acyclic aliphatic; optionally substituted, cyclic or acyclicheteroaliphatic; optionally substituted aryl; or optionally substitutedheteroaryl;

X′ is O or NR_(y), wherein R_(y) is hydrogen or optionally substitutedaliphatic;

each m independently is an integer between 1 and 100, inclusive;

n is an integer between 1 and 10,000, inclusive.

In certain embodiments, provided herein are polymers of Formulae (II),(III), (IV), and (V), and pharmaceutically acceptable salts thereof:

In another aspect, provided herein is a polylactone diacrylate compoundof Formula (VI):

wherein:

R is optionally substituted, cyclic or acyclic aliphatic; optionallysubstituted, cyclic or acyclic heteroaliphatic; or a combinationthereof;

each R′ independently is optionally substituted, cyclic or acyclicaliphatic; optionally substituted, cyclic or acyclic heteroaliphatic; ora combination thereof;

each R_(a) independently is hydrogen or optionally substitutedaliphatic; and

m is an integer between 1 and 100, inclusive.

In another aspect, the present disclosure provides methods of preparingpolymers of Formulae (II), (III), (IV), and (V). In another aspect, thepresent disclosure provides methods of preparing compounds of Formula(VI).

In another aspect, the present disclosure provides compositionscomprising a polymer described herein. In certain embodiments, thecomposition further comprises an agent, e.g., a small organic molecule,inorganic molecule, nucleic acid, protein, peptide, or polynucleotide.

In another aspect, provided herein are methods of treating a disease,disorder, or condition from which a subject suffers comprisingadministering to the subject in need thereof an effective amount of acomposition described herein.

In another aspect, provided herein are methods of delivering apolynucleotide to a cell comprising contacting the cell with acomposition described herein.

In another aspect, the present disclosure provides a kit comprising oneor more components selected from the polymers and compounds describedherein.

The details of certain embodiments of the invention are set forth in theDetailed Description of Certain Embodiments, as described below. Otherfeatures, objects, and advantages of the invention will be apparent fromthe Definitions, Figures, Examples, and Claims.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the general synthesis of PBAE polymers.

FIG. 2 shows the biodegradation products generated by the hydrolysis ofthe PBAE polymers.

FIG. 3 shows the structure of 1,4-butanediol diacrylate and Bisphenol Aglycerolate diacrylate (DD).

FIG. 4 shows the synthesis of poly-caprolactone-based diacrylates.

FIG. 5 shows an embodiment of the synthesis of PBAE polymers usingexemplary amines and diacrylates.

FIG. 6 shows exemplary PCL-based diacrylates used in the synthesis ofPBAE polymers.

FIG. 7 shows exemplary amines used in the synthesis of PBAE polymers.

FIGS. 8A to 8B show: (FIG. 8A) The characterization data for diacrylatesA, B, C, and D. (FIG. 8B) ¹H NMR spectrum and proton peak assignments ofdiacrylate C.

FIG. 9 shows the gel permeation chromatography (GPC) characterization ofPBAE polymers prepared from amines (1, 2, 3, 4) and diacrylates (A, B,C, D).

FIGS. 10A to 10D show: (FIG. 10A) M_(w) of the custom diacrylates andthe PBAE polymers with different hydrophilic amines. (FIG. 10B)Transfection in HeLA cells of a PBAE series using direct mixing orpre-mixing protocol. (FIG. 10C) Transfection in HeLA cells using onlypre-mixing protocol. (FIG. 10D) HeLa cell relative viability for all thesynthesized PBAE polymers.

FIG. 11 shows NP PEG-lipid distributions of B2 for the primary andsecondary NPs obtained in the direct mixing protocol and NP D_(n)distribution of the same PBAE with the pre-mixing protocol.

FIG. 12A shows the characterization data of selected mRNA-containingPBAE nanoparticles containing 0% PEG. FIG. 12B shows thecharacterization data of selected mRNA-containing PBAE nanoparticlescontaining 15% PEG.

FIGS. 13A to 13F show: (FIG. 13A) Serum stability of PBAE polymers with5 CL unit (C) at 7 wt. % of PEG-lipid. (FIG. 13B) Serum stability of C1at different wt. % of PEG-lipid. (FIG. 13C) Image of C1 24 hours afterIV injection (0.5 mg/kg mRNA). (FIG. 13D) Liver enzyme activity of C1and Jet PEI at a dose of 0.5 mg/kg. (FIG. 13E) Average radiance of C1and JET PEI in the spleen after 24 hours at different mRNA doses. (FIG.13F) Average radiance of C1 and JET PEI in the lungs after 24 hours atdifferent mRNA doses.

FIGS. 14A to 14B show: (FIG. 14A) Transfection efficiency of PBAE 2series with varying percentages of PEG-lipid. (FIG. 14B) Transfectionefficiency of all PBAEs with 15 wt. % PEG-lipid.

FIG. 15 shows percent weight gain for C1 nanoparticles. Data arepresented as mean±SD, n=3.

DEFINITIONS

For convenience, certain terms employed herein, in the specification,examples, and claims are collected herein.

Unless otherwise required by context, singular terms shall includepluralities, and plural terms shall include the singular.

The following definitions are more general terms used throughout thepresent application:

The singular terms “a,” “an,” and “the” include plural references unlessthe context clearly indicates otherwise. Similarly, the word “or” isintended to include “and” unless the context clearly indicatesotherwise.

Other than in the examples, or where otherwise indicated, all numbersexpressing quantities of ingredients or reaction conditions used hereinshould be understood as modified in all instances by the term “about.”“About” and “approximately” shall generally mean an acceptable degree oferror for the quantity measured given the nature or precision of themeasurements. Exemplary degrees of error are within 20 percent (%),typically, within 10%, or more typically, within 5%, 4%, 3%, 2%, or 1%of a given value or range of values.

Definitions of specific functional groups and chemical terms aredescribed in more detail below. The chemical elements are identified inaccordance with the Periodic Table of the Elements, CAS version,Handbook of Chemistry and Physics, 75^(th) Ed., inside cover, andspecific functional groups are generally defined as described therein.Additionally, general principles of organic chemistry, as well asspecific functional moieties and reactivity, are described in OrganicChemistry, Thomas Sorrell, University Science Books, Sausalito, 1999;Smith and March March's Advanced Organic Chemistry, 5^(th) Edition, JohnWiley & Sons, Inc., New York, 2001; Larock, Comprehensive OrganicTransformations, VCH Publishers, Inc., New York, 1989; and Carruthers,Some Modern Methods of Organic Synthesis, 3^(rd) Edition, CambridgeUniversity Press, Cambridge, 1987.

Compounds described herein can include one or more asymmetric centers,and thus can exist in various stereoisomeric forms, e.g., enantiomersand/or diastereomers. For example, the compounds described herein can bein the form of an individual enantiomer, diastereomer or geometricisomer, or can be in the form of a mixture of stereoisomers, includingracemic mixtures and mixtures enriched in one or more stereoisomer.Isomers can be isolated from mixtures by methods known to those skilledin the art, including chiral high pressure liquid chromatography (HPLC)and the formation and crystallization of chiral salts; or preferredisomers can be prepared by asymmetric syntheses. See, for example,Jacques et al., Enantiomers, Racemates and Resolutions (WileyInterscience, New York, 1981); Wilen et al., Tetrahedron 33:2725 (1977);Eliel, E. L. Stereochemistry of Carbon Compounds (McGraw-Hill, N Y,1962); and Wilen, S. H. Tables of Resolving Agents and OpticalResolutions p. 268 (E. L. Eliel, Ed., Univ. of Notre Dame Press, NotreDame, Ind. 1972). The disclosure additionally encompasses compounds asindividual isomers substantially free of other isomers, andalternatively, as mixtures of various isomers.

In a chemical formula,

represents a bond that is absent or a single bond, and

or

each represent a single or double bond.

When a range of values is listed, it is intended to encompass each valueand sub-range within the range. For example “C₁-C₆ alkyl” is intended toencompass, C₁, C₂, C₃, C₄, C₅, C₆, C₁-C₆, C₁-C₅, C₁-C₄, C₁-C₃, C₁-C₂,C₂-C₆, C₂-C₅, C₂-C₄, C₂-C₃, C₃-C₆, C₃-C₅, C₃-C₄, C₄-C₆, C₄-C₅, and C₅-C₆alkyl.

The term “aliphatic” includes both saturated and unsaturated, straightchain (i.e., unbranched), branched, acyclic, cyclic, or polycyclicaliphatic hydrocarbons, which are optionally substituted with one ormore functional groups. Likewise, the term “heteroaliphatic” refers toheteroalkyl, heteroalkenyl, heteroalkynyl, and heterocyclic groups. Aswill be appreciated by one of ordinary skill in the art, “aliphatic” isintended herein to include, but is not limited to, alkyl, alkenyl,alkynyl, cycloalkyl, cycloalkenyl, and cycloalkynyl moieties. Thus, theterm “alkyl” includes straight, branched and cyclic alkyl groups. Ananalogous convention applies to other generic terms such as “alkenyl”,“alkynyl”, and the like. Furthermore, the terms “alkyl”, “alkenyl”,“alkynyl”, and the like encompass both substituted and unsubstitutedgroups. In certain embodiments, “lower alkyl” is used to indicate thosealkyl groups (cyclic, acyclic, substituted, unsubstituted, branched, orunbranched) having 1-6 carbon atoms.

In certain embodiments, the alkyl, alkenyl, and alkynyl groups employedin the disclosure contain 1-100 aliphatic carbon atoms. In certain otherembodiments, the alkyl, alkenyl, and alkynyl groups employed in thedisclosure contain 1-50 aliphatic carbon atoms. In certain otherembodiments, the alkyl, alkenyl, and alkynyl groups employed in thedisclosure contain 1-20 aliphatic carbon atoms. In certain otherembodiments, the alkyl, alkenyl, and alkynyl groups employed in thedisclosure contain 1-10 aliphatic carbon atoms. In yet otherembodiments, the alkyl, alkenyl, and alkynyl groups employed in thedisclosure contain 1-8 aliphatic carbon atoms. In still otherembodiments, the alkyl, alkenyl, and alkynyl groups employed in thedisclosure contain 1-6 aliphatic carbon atoms. In yet other embodiments,the alkyl, alkenyl, and alkynyl groups employed in the disclosurecontain 1-4 carbon atoms. Illustrative aliphatic groups thus include,but are not limited to, for example, methyl, ethyl, npropyl, isopropyl,cyclopropyl, —CH₂-cyclopropyl, vinyl, allyl, n-butyl, sec-butyl,isobutyl, tertbutyl, cyclobutyl, —CH₂-cyclobutyl, n-pentyl, sec-pentyl,isopentyl, tert-pentyl, cyclopentyl, —CH₂-cyclopentyl, n-hexyl,sec-hexyl, cyclohexyl, —CH₂-cyclohexyl, and the like, which again maybear one or more substituents. Alkenyl groups include, but are notlimited to, for example, ethenyl, propenyl, butenyl,1-methyl-2-buten-1-yl, and the like. Representative alkynyl groupsinclude, but are not limited to, ethynyl, 2-propynyl (propargyl),1-propynyl, and the like.

The term “alkyl” refers to a radical of a straight-chain or branchedsaturated hydrocarbon group. In some embodiments, an alkyl group has 1to 1000 carbon atoms (“C₁-C₁₀₀₀ alkyl”), 1 to 900 carbon atoms (“C₁-C₉₀₀alkyl”), 1 to 800 carbon atoms (“C₁-C₈₀₀ alkyl”), 1 to 700 carbon atoms(“C₁-C₇₀₀ alkyl”), 1 to 600 carbon atoms (“C₁-C₆₀₀ alkyl”), 1 to 500carbon atoms (“C₁-C₅₀₀ alkyl”), 1 to 400 carbon atoms (“C₁-C₄₀₀ alkyl”),1 to 300 carbon atoms (“C₁-C₃₀₀ alkyl”), 1 to 200 carbon atoms (“C₁-C₂₀₀alkyl”), 1 to 100 carbon atom (“C₁-C₁₀₀ alkyl”). In some embodiments, analkyl group has 1 to 10 carbon atoms (“C₁-C₁₀ alkyl”), 1 to 9 carbonatoms (“C₁-C₉ alkyl”), 1 to 8 carbon atoms (“C₁-C₈ alkyl”), 1 to 7carbon atoms (“C₁-C₇ alkyl”), 1 to 6 carbon atoms (“C₁-C₆ alkyl”), 1 to5 carbon atoms (“C₁-C₅ alkyl”), 1 to 4 carbon atoms (“C₁-C₄ alkyl”), 1to 3 carbon atoms (“C₁-C₃ alkyl”), 1 to 2 carbon atoms (“C₁-C₂ alkyl”),or 1 carbon atom (“C₁ alkyl”). Examples of C₁-C₆ alkyl groups includemethyl (C₁), ethyl (C₂), n-propyl (C₃), isopropyl (C₃), n-butyl (C₄),tert-butyl (C₄), sec-butyl (C₄), iso-butyl (C₄), n-pentyl (C₅),3-pentanyl (C₅), amyl (C₅), neopentyl (C₅), 3-methyl-2-butanyl (C₅),tertiary amyl (C₅), and n-hexyl (C₆). Additional examples of alkylgroups include n-heptyl (C₇), n-octyl (C₈) and the like. Unlessotherwise specified, each instance of an alkyl group is independentlyunsubstituted (an “unsubstituted alkyl”) or substituted (a “substitutedalkyl”) with one or more substituents.

The term “alkenyl” refers to a radical of a straight-chain or branchedhydrocarbon group having from 2 to 1000 carbon atoms and one or morecarbon-carbon double bonds (e.g., 1, 2, 3, or 4 double bonds). In someembodiments, an alkenyl group has 2 to 1000 carbon atoms (“C₂-C₁₀₀₀alkenyl”), 2 to 900 carbon atoms (“C₂-C₉₀₀ alkenyl”), 2 to 800 carbonatoms (“C₂-C₈₀₀ alkenyl”), 2 to 700 carbon atoms (“C₂-C₇₀₀ alkenyl”), 2to 600 carbon atoms (“C₂-C₆₀₀ alkenyl”), 2 to 500 carbon atoms (“C₂-C₅₀₀alkenyl”), 2 to 400 carbon atoms (“C₂-C₄₀₀ alkenyl”), 2 to 300 carbonatoms (“C₂-C₃₀₀ alkenyl”), 2 to 200 carbon atoms (“C₂-C₂₀₀ alkenyl”), 2to 100 carbon atom (“C₂-C₁₀₀ alkenyl”). In some embodiments, an alkenylgroup has 2 to 9 carbon atoms (“C₂₋₉ alkenyl”). In some embodiments, analkenyl group has 2 to 8 carbon atoms (“C₂₋₈ alkenyl”). In someembodiments, an alkenyl group has 2 to 7 carbon atoms (“C₂₋₇ alkenyl”).In some embodiments, an alkenyl group has 2 to 6 carbon atoms (“C₂₋₆alkenyl”). In some embodiments, an alkenyl group has 2 to 5 carbon atoms(“C₂₋₅ alkenyl”). In some embodiments, an alkenyl group has 2 to 4carbon atoms (“C₂₋₄ alkenyl”). In some embodiments, an alkenyl group has2 to 3 carbon atoms (“C₂₋₃ alkenyl”). In some embodiments, an alkenylgroup has 2 carbon atoms (“C₂ alkenyl”). The one or more carbon-carbondouble bonds can be internal (such as in 2-butenyl) or terminal (such asin 1-butenyl). Examples of C₂₋₄ alkenyl groups include ethenyl (C₂),1-propenyl (C₃), 2-propenyl (C₃), 1-butenyl (C₄), 2-butenyl (C₄),butadienyl (C₄), and the like. Examples of C₂₋₆ alkenyl groups includethe aforementioned C₂₋₄ alkenyl groups as well as pentenyl (C₅),pentadienyl (C₅), hexenyl (C₆), and the like. Unless otherwisespecified, each instance of an alkenyl group is independentlyunsubstituted (an “unsubstituted alkenyl”) or substituted (a“substituted alkenyl”) with one or more substituents. In an alkenylgroup, a C═C double bond for which the stereochemistry is not specified(e.g., —CH═CHCH₃

may be in the (E)- or (Z)-configuration.

The term “alkynyl” refers to a radical of a straight-chain or branchedhydrocarbon group having from 2 to 1000 carbon atoms and one or morecarbon-carbon triple bonds (e.g., 1, 2, 3, or 4 triple bonds). In someembodiments, an alkynyl group has 2 to 1000 carbon atoms (“C₂-C₁₀₀₀alkynyl”), 2 to 900 carbon atoms (“C₂-C₉₀₀ alkynyl”), 2 to 800 carbonatoms (“C₂-C₈₀₀ alkynyl”), 2 to 700 carbon atoms (“C₂-C₇₀₀ alkynyl”), 2to 600 carbon atoms (“C₂-C₆₀₀ alkynyl”), 2 to 500 carbon atoms (“C₂-C₅₀₀alkynyl”), 2 to 400 carbon atoms (“C₂-C₄₀₀ alkynyl”), 2 to 300 carbonatoms (“C₂-C₃₀₀ alkynyl”), 2 to 200 carbon atoms (“C₂-C₂₀₀ alkynyl”), 2to 100 carbon atom (“C₂-C₁₀₀ alkynyl”). In some embodiments, an alkynylgroup has 2 to 9 carbon atoms (“C₂₋₉ alkynyl”), 2 to 8 carbon atoms(“C₂₋₈ alkynyl”), 2 to 7 carbon atoms (“C₂₋₇ alkynyl”), 2 to 6 carbonatoms (“C₂₋₆ alkynyl”), 2 to 5 carbon atoms (“C₂₋₅ alkynyl”), 2 to 4carbon atoms (“C₂₋₄ alkynyl”), 2 to 3 carbon atoms (“C₂₋₃ alkynyl”), or2 carbon atoms (“C₂ alkynyl”). The one or more carbon-carbon triplebonds can be internal (such as in 2-butynyl) or terminal (such as in1-butynyl). Examples of C₂₋₄ alkynyl groups include, without limitation,ethynyl (C₂), 1-propynyl (C₃), 2-propynyl (C₃), 1-butynyl (C₄),2-butynyl (C₄), and the like. Examples of C₂₋₆ alkenyl groups includethe aforementioned C₂₋₄ alkynyl groups as well as pentynyl (C₅), hexynyl(C₆), and the like. Unless otherwise specified, each instance of analkynyl group is independently unsubstituted (an “unsubstitutedalkynyl”) or substituted (a “substituted alkynyl”) with one or moresubstituents.

The term “heteroalkyl” refers to an alkyl group which further includesat least one heteroatom (e.g., 1, 2, 3, or 4 heteroatoms) selected fromoxygen, nitrogen, phosphorus, or sulfur within (i.e., inserted betweenadjacent carbon atoms of) and/or placed at one or more terminalposition(s) of the parent chain. In certain embodiments, a heteroalkylgroup refers to a saturated group having from 1 to 1000 carbon atoms and1 or more heteroatoms within the parent chain (“C₁-C₁₀₀₀ heteroalkyl”),1 to 900 carbon atoms and 1 or more heteroatoms within the parent chain(“C₁-C₉₀₀ heteroalkyl”), 1 to 800 carbon atoms and 1 or more heteroatomswithin the parent chain (“C₁-C₈₀₀ heteroalkyl”), 1 to 700 carbon atomsand 1 or more heteroatoms within the parent chain (“C₁-C₇₀₀heteroalkyl”), 1 to 600 carbon atoms and 1 or more heteroatoms withinthe parent chain (“C₁-C₆₀₀ heteroalkyl”), 1 to 500 carbon atoms and 1 ormore heteroatoms within the parent chain (“C₁-C₅₀₀ heteroalkyl”), 1 to400 carbon atoms and 1 or more heteroatoms within the parent chain(“C₁-C₄₀₀ heteroalkyl”), 1 to 300 carbon atoms and 1 or more heteroatomswithin the parent chain (“C₁-C₃₀₀ heteroalkyl”), 1 to 200 carbon atomsand 1 or more heteroatoms within the parent chain (“C₁-C₂₀₀heteroalkyl”), or 1 to 100 carbon atoms and 1 or more heteroatoms withinthe parent chain (“C₁-C₁₀₀ heteroalkyl”). In certain embodiments, aheteroalkyl group refers to a saturated group having from 1 to 10 carbonatoms and 1 or more heteroatoms within the parent chain (“C₁-C₁₀heteroalkyl”), 1 to 9 carbon atoms and 1 or more heteroatoms within theparent chain (“C₁-C₉ heteroalkyl”), 1 to 8 carbon atoms and 1 or moreheteroatoms within the parent chain (“C₁-C₈ heteroalkyl”), 1 to 7 carbonatoms and 1 or more heteroatoms within the parent chain (“C₁-C₇heteroalkyl”), 1 to 6 carbon atoms and 1 or more heteroatoms within theparent chain (“C₁-C₆ heteroalkyl”), 1 to 5 carbon atoms and 1 or moreheteroatoms within the parent chain (“C₁-C₅ heteroalkyl”), 1 to 4 carbonatoms and 1 or more heteroatoms within the parent chain (“C₁-C₄heteroalkyl”), 1 to 3 carbon atoms and 1 or more heteroatoms within theparent chain (“C₁-C₃ heteroalkyl”), 1 to 2 carbon atoms and 1 heteroatomwithin the parent chain (“C₁-C₂ heteroalkyl”), or 1 carbon atom and 1heteroatom (“C₁ heteroalkyl”). Unless otherwise specified, each instanceof a heteroalkyl group is independently unsubstituted (an “unsubstitutedheteroalkyl”) or substituted (a “substituted heteroalkyl”) with one ormore substituents.

The term “heteroalkenyl” refers to an alkenyl group, which furtherincludes at least one heteroatom (e.g., 1, 2, 3, or 4 heteroatoms)selected from oxygen, nitrogen, or sulfur within (i.e., inserted betweenadjacent carbon atoms of) and/or placed at one or more terminalposition(s) of the parent chain. In certain embodiments, a heteroalkenylgroup refers to a saturated group having from 1 to 1000 carbon atoms and1 or more heteroatoms within the parent chain (“heteroC₁-C₁₀₀₀alkenyl”), 1 to 900 carbon atoms and 1 or more heteroatoms within theparent chain (“heteroC₁-C₉₀₀ alkenyl”), 1 to 800 carbon atoms and 1 ormore heteroatoms within the parent chain (“heteroC₁-C₈₀₀ alkenyl”), 1 to700 carbon atoms and 1 or more heteroatoms within the parent chain(“heteroC₁-C₇₀₀ alkenyl”), 1 to 600 carbon atoms and 1 or moreheteroatoms within the parent chain (“heteroC₁-C₆₀₀ alkenyl”), 1 to 500carbon atoms and 1 or more heteroatoms within the parent chain(“heteroC₁-C₅₀₀ alkenyl”), 1 to 400 carbon atoms and 1 or moreheteroatoms within the parent chain (“heteroC₁-C₄₀₀ alkenyl”), 1 to 300carbon atoms and 1 or more heteroatoms within the parent chain(“heteroC₁-C₃₀₀ alkenyl”), 1 to 200 carbon atoms and 1 or moreheteroatoms within the parent chain (“heteroC₁-C₂₀₀ alkenyl”), or 1 to100 carbon atoms and 1 or more heteroatoms within the parent chain(“heteroC₁-C₁₀₀ alkenyl”). In certain embodiments, a heteroalkenyl grouprefers to a group having from 2 to 10 carbon atoms, at least one doublebond, and 1 or more heteroatoms within the parent chain (“heteroC₂₋₁₀alkenyl”). In some embodiments, a heteroalkenyl group has 2 to 9 carbonatoms at least one double bond, and 1 or more heteroatoms within theparent chain (“heteroC₂₋₉ alkenyl”). In some embodiments, aheteroalkenyl group has 2 to 8 carbon atoms, at least one double bond,and 1 or more heteroatoms within the parent chain (“heteroC₂₋₈alkenyl”). In some embodiments, a heteroalkenyl group has 2 to 7 carbonatoms, at least one double bond, and 1 or more heteroatoms within theparent chain (“heteroC₂₋₇ alkenyl”). In some embodiments, aheteroalkenyl group has 2 to 6 carbon atoms, at least one double bond,and 1 or more heteroatoms within the parent chain (“heteroC₂₋₆alkenyl”). In some embodiments, a heteroalkenyl group has 2 to 5 carbonatoms, at least one double bond, and 1 or 2 heteroatoms within theparent chain (“heteroC₂₋₅ alkenyl”). In some embodiments, aheteroalkenyl group has 2 to 4 carbon atoms, at least one double bond,and 1 or 2 heteroatoms within the parent chain (“heteroC₂₋₄ alkenyl”).In some embodiments, a heteroalkenyl group has 2 to 3 carbon atoms, atleast one double bond, and 1 heteroatom within the parent chain(“heteroC₂₋₃ alkenyl”). In some embodiments, a heteroalkenyl group has 2to 6 carbon atoms, at least one double bond, and 1 or 2 heteroatomswithin the parent chain (“heteroC₂₋₆ alkenyl”). Unless otherwisespecified, each instance of a heteroalkenyl group is independentlyunsubstituted (an “unsubstituted heteroalkenyl”) or substituted (a“substituted heteroalkenyl”) with one or more substituents. In certainembodiments, the heteroalkenyl group is an unsubstituted heteroC₂₋₁₀alkenyl. In certain embodiments, the heteroalkenyl group is asubstituted heteroC₂₋₁₀ alkenyl.

The term “heteroalkynyl” refers to an alkynyl group, which furtherincludes at least one heteroatom (e.g., 1, 2, 3, or 4 heteroatoms)selected from oxygen, nitrogen, or sulfur within (i.e., inserted betweenadjacent carbon atoms of) and/or placed at one or more terminalposition(s) of the parent chain. In certain embodiments, a heteroalkynylgroup refers to a saturated group having from 1 to 1000 carbon atoms and1 or more heteroatoms within the parent chain (“heteroC₁-C₁₀₀₀alkynyl”), 1 to 900 carbon atoms and 1 or more heteroatoms within theparent chain (“heteroC₁-C₉₀₀ alkynyl”), 1 to 800 carbon atoms and 1 ormore heteroatoms within the parent chain (“heteroC₁-C₈₀₀ alkynyl”), 1 to700 carbon atoms and 1 or more heteroatoms within the parent chain(“heteroC₁-C₇₀₀ alkynyl), 1 to 600 carbon atoms and 1 or moreheteroatoms within the parent chain (“heteroC₁-C₆₀₀ alkynyl”), 1 to 500carbon atoms and 1 or more heteroatoms within the parent chain(“heteroC₁-C₅₀₀ alkynyl”), 1 to 400 carbon atoms and 1 or moreheteroatoms within the parent chain (“heteroC₁-C₄₀₀ alkynyl”), 1 to 300carbon atoms and 1 or more heteroatoms within the parent chain(“heteroC₁-C₃₀₀ alkynyl”), 1 to 200 carbon atoms and 1 or moreheteroatoms within the parent chain (“heteroC₁-C₂₀₀ alkynyl”), or 1 to100 carbon atoms and 1 or more heteroatoms within the parent chain(“heteroC₁-C₁₀₀ alkynyl”). In certain embodiments, a heteroalkynyl grouprefers to a group having from 2 to 10 carbon atoms, at least one triplebond, and 1 or more heteroatoms within the parent chain (“heteroC₂₋₁₀alkynyl”). In some embodiments, a heteroalkynyl group has 2 to 9 carbonatoms, at least one triple bond, and 1 or more heteroatoms within theparent chain (“heteroC₂₋₉ alkynyl”). In some embodiments, aheteroalkynyl group has 2 to 8 carbon atoms, at least one triple bond,and 1 or more heteroatoms within the parent chain (“heteroC₂₋₈alkynyl”). In some embodiments, a heteroalkynyl group has 2 to 7 carbonatoms, at least one triple bond, and 1 or more heteroatoms within theparent chain (“heteroC₂₋₇ alkynyl”). In some embodiments, aheteroalkynyl group has 2 to 6 carbon atoms, at least one triple bond,and 1 or more heteroatoms within the parent chain (“heteroC₂₋₆alkynyl”). In some embodiments, a heteroalkynyl group has 2 to 5 carbonatoms, at least one triple bond, and 1 or 2 heteroatoms within theparent chain (“heteroC₂₋₅ alkynyl”). In some embodiments, aheteroalkynyl group has 2 to 4 carbon atoms, at least one triple bond,and 1 or 2 heteroatoms within the parent chain (“heteroC₂₋₄ alkynyl”).In some embodiments, a heteroalkynyl group has 2 to 3 carbon atoms, atleast one triple bond, and 1 heteroatom within the parent chain(“heteroC₂₋₃ alkynyl”). In some embodiments, a heteroalkynyl group has 2to 6 carbon atoms, at least one triple bond, and 1 or 2 heteroatomswithin the parent chain (“heteroC₂₋₆ alkynyl”). Unless otherwisespecified, each instance of a heteroalkynyl group is independentlyunsubstituted (an “unsubstituted heteroalkynyl”) or substituted (a“substituted heteroalkynyl”) with one or more substituents. In certainembodiments, the heteroalkynyl group is an unsubstituted heteroC₂₋₁₀alkynyl. In certain embodiments, the heteroalkynyl group is asubstituted heteroC₂₋₁₀ alkynyl.

The term “carbocyclyl” or “carbocyclic” or “cycloalkyl” refers to aradical of a non-aromatic cyclic hydrocarbon group having from 3 to 10ring carbon atoms (“C₃₋₁₀ carbocyclyl”) and zero heteroatoms in thenon-aromatic ring system. In some embodiments, a carbocyclyl group has 3to 8 ring carbon atoms (“C₃₋₈ carbocyclyl”), 3 to 7 ring carbon atoms(“C₃₋₇ carbocyclyl”), 3 to 6 ring carbon atoms (“C₃₋₆ carbocyclyl”), 4to 6 ring carbon atoms (“C₄₋₆ carbocyclyl”), 5 to 6 ring carbon atoms(“C₅₋₆ carbocyclyl”), or 5 to 10 ring carbon atoms (“C₅₋₁₀carbocyclyl”). Exemplary C₃₋₆ carbocyclyl groups include, withoutlimitation, cyclopropyl (C₃), cyclopropenyl (C₃), cyclobutyl (C₄),cyclobutenyl (C₄), cyclopentyl (C₅), cyclopentenyl (C₅), cyclohexyl(C₆), cyclohexenyl (C₆), cyclohexadienyl (C₆), and the like. ExemplaryC₃₋₈ carbocyclyl groups include, without limitation, the aforementionedC₃₋₆ carbocyclyl groups as well as cycloheptyl (C₇), cycloheptenyl (C₇),cycloheptadienyl (C₇), cycloheptatrienyl (C₇), cyclooctyl (C₈),cyclooctenyl (C₈), bicyclo[2.2.1]heptanyl (C₇), bicyclo[2.2.2]octanyl(C₈), and the like. Exemplary C₃₋₁₀ carbocyclyl groups include, withoutlimitation, the aforementioned C₃₋₈ carbocyclyl groups as well ascyclononyl (C₉), cyclononenyl (C₉), cyclodecyl (C₁₀), cyclodecenyl(C₁₀), octahydro-1H-indenyl (C₉), decahydronaphthalenyl (C₁₀),spiro[4.5]decanyl (C₁₀), and the like. As the foregoing examplesillustrate, in certain embodiments, the carbocyclyl group is eithermonocyclic (“monocyclic carbocyclyl”) or polycyclic (e.g., containing afused, bridged or spiro ring system such as a bicyclic system (“bicycliccarbocyclyl”) or tricyclic system (“tricyclic carbocyclyl”)) and can besaturated or can contain one or more carbon-carbon double or triplebonds. “Carbocyclyl” also includes ring systems wherein the carbocyclylring, as defined above, is fused with one or more aryl or heteroarylgroups wherein the point of attachment is on the carbocyclyl ring, andin such instances, the number of carbons continue to designate thenumber of carbons in the carbocyclic ring system. Unless otherwisespecified, each instance of a carbocyclyl group is independentlyunsubstituted (an “unsubstituted carbocyclyl”) or substituted (a“substituted carbocyclyl”) with one or more substituents.

The term “heterocyclyl” or “heterocyclic” refers to a radical of a 3- to14-membered non-aromatic ring system having ring carbon atoms and 1 to 4ring heteroatoms, wherein each heteroatom is independently selected fromnitrogen, oxygen, phosphorus, and sulfur (“3-14 membered heterocyclyl”).In heterocyclyl groups that contain one or more nitrogen atoms, thepoint of attachment can be a carbon or nitrogen atom, as valencypermits. A heterocyclyl group can either be monocyclic (“monocyclicheterocyclyl”) or polycyclic (e.g., a fused, bridged or spiro ringsystem such as a bicyclic system (“bicyclic heterocyclyl”) or tricyclicsystem (“tricyclic heterocyclyl”)), and can be saturated or can containone or more carbon-carbon double or triple bonds. Heterocyclylpolycyclic ring systems can include one or more heteroatoms in one orboth rings. “Heterocyclyl” also includes ring systems wherein theheterocyclyl ring, as defined above, is fused with one or morecarbocyclyl groups wherein the point of attachment is either on thecarbocyclyl or heterocyclyl ring, or ring systems wherein theheterocyclyl ring, as defined above, is fused with one or more aryl orheteroaryl groups, wherein the point of attachment is on theheterocyclyl ring, and in such instances, the number of ring memberscontinue to designate the number of ring members in the heterocyclylring system. Unless otherwise specified, each instance of heterocyclylis independently unsubstituted (an “unsubstituted heterocyclyl”) orsubstituted (a “substituted heterocyclyl”) with one or moresubstituents.

In some embodiments, a heterocyclyl group is a 5-10 memberednon-aromatic ring system having ring carbon atoms and 1-4 ringheteroatoms, wherein each heteroatom is independently selected fromnitrogen, oxygen, phosphorus, and sulfur (“5-10 membered heterocyclyl”).In some embodiments, a heterocyclyl group is a 5-8 membered non-aromaticring system having ring carbon atoms and 1-4 ring heteroatoms, whereineach heteroatom is independently selected from nitrogen, oxygen,phosphorus, and sulfur (“5-8 membered heterocyclyl”). In someembodiments, a heterocyclyl group is a 5-6 membered non-aromatic ringsystem having ring carbon atoms and 1-4 ring heteroatoms, wherein eachheteroatom is independently selected from nitrogen, oxygen, phosphorus,and sulfur (“5-6 membered heterocyclyl”). In some embodiments, the 5-6membered heterocyclyl has 1-3 ring heteroatoms selected from nitrogen,oxygen, phosphorus, and sulfur. In some embodiments, the 5-6 memberedheterocyclyl has 1-2 ring heteroatoms selected from nitrogen, oxygen,phosphorus, and sulfur. In some embodiments, the 5-6 memberedheterocyclyl has 1 ring heteroatom selected from nitrogen, oxygen,phosphorus, and sulfur.

Exemplary 3-membered heterocyclyl groups containing 1 heteroatominclude, without limitation, azirdinyl, oxiranyl, and thiiranyl.Exemplary 4-membered heterocyclyl groups containing 1 heteroatominclude, without limitation, azetidinyl, oxetanyl and thietanyl.Exemplary 5-membered heterocyclyl groups containing 1 heteroatominclude, without limitation, tetrahydrofuranyl, dihydrofuranyl,tetrahydrothiophenyl, dihydrothiophenyl, pyrrolidinyl, dihydropyrrolyl,and pyrrolyl-2,5-dione. Exemplary 5-membered heterocyclyl groupscontaining 2 heteroatoms include, without limitation, dioxolanyl,oxathiolanyl and dithiolanyl. Exemplary 5-membered heterocyclyl groupscontaining 3 heteroatoms include, without limitation, triazolinyl,oxadiazolinyl, and thiadiazolinyl. Exemplary 6-membered heterocyclylgroups containing 1 heteroatom include, without limitation, piperidinyl,tetrahydropyranyl, dihydropyridinyl, and thianyl. Exemplary 6-memberedheterocyclyl groups containing 2 heteroatoms include, withoutlimitation, piperazinyl, morpholinyl, dithianyl, and dioxanyl. Exemplary6-membered heterocyclyl groups containing 3 heteroatoms include, withoutlimitation, triazinanyl. Exemplary 7-membered heterocyclyl groupscontaining 1 heteroatom include, without limitation, azepanyl, oxepanyl,and thiepanyl. Exemplary 8-membered heterocyclyl groups containing 1heteroatom include, without limitation, azocanyl, oxecanyl andthiocanyl. Exemplary bicyclic heterocyclyl groups include, withoutlimitation, indolinyl, isoindolinyl, dihydrobenzofuranyl,dihydrobenzothienyl, tetrahydrobenzothienyl, tetrahydrobenzofuranyl,tetrahydroindolyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl,decahydroquinolinyl, decahydroisoquinolinyl, octahydrochromenyl,octahydroisochromenyl, decahydronaphthyridinyl,decahydro-1,8-naphthyridinyl, octahydropyrrolo[3,2-b]pyrrole, indolinyl,phthalimidyl, naphthalimidyl, chromanyl, chromenyl,1H-benzo[e][1,4]diazepinyl, 1,4,5,7-tetrahydropyrano[3,4-b]pyrrolyl,5,6-dihydro-4H-furo[3,2-b]pyrrolyl, 6,7-dihydro-5H-furo[3,2-b]pyranyl,5,7-dihydro-4H-thieno[2,3-c]pyranyl,2,3-dihydro-1H-pyrrolo[2,3-b]pyridinyl, 2,3-dihydrofuro[2,3-b]pyridinyl,4,5,6,7-tetrahydro-1H-pyrrolo[2,3-b]pyridinyl,4,5,6,7-tetrahydrofuro[3,2-c]pyridinyl,4,5,6,7-tetrahydrothieno[3,2-b]pyridinyl,1,2,3,4-tetrahydro-1,6-naphthyridinyl, and the like.

The term “aryl” refers to a radical of a monocyclic or polycyclic (e.g.,bicyclic or tricyclic) 4n+2 aromatic ring system (e.g., having 6, 10, or14 π electrons shared in a cyclic array) having 6-14 ring carbon atomsand zero heteroatoms provided in the aromatic ring system (“C₆₋₁₄aryl”). In some embodiments, an aryl group has 6 ring carbon atoms (“C₆aryl”; e.g., phenyl). In some embodiments, an aryl group has 10 ringcarbon atoms (“C₁₀ aryl”; e.g., naphthyl such as 1-naphthyl and2-naphthyl). In some embodiments, an aryl group has 14 ring carbon atoms(“C₁₄ aryl”; e.g., anthracyl). “Aryl” also includes ring systems whereinthe aryl ring, as defined above, is fused with one or more carbocyclylor heterocyclyl groups wherein the radical or point of attachment is onthe aryl ring, and in such instances, the number of carbon atomscontinue to designate the number of carbon atoms in the aryl ringsystem. Unless otherwise specified, each instance of an aryl group isindependently unsubstituted (an “unsubstituted aryl”) or substituted (a“substituted aryl”) with one or more substituents.

The term “heteroaryl” refers to a radical of a 5-14 membered monocyclicor polycyclic (e.g., bicyclic, tricyclic) 4n+2 aromatic ring system(e.g., having 6, 10, or 14 π electrons shared in a cyclic array) havingring carbon atoms and 1-4 ring heteroatoms provided in the aromatic ringsystem, wherein each heteroatom is independently selected from nitrogen,oxygen, and sulfur (“5-14 membered heteroaryl”). In heteroaryl groupsthat contain one or more nitrogen atoms, the point of attachment can bea carbon or nitrogen atom, as valency permits. Heteroaryl polycyclicring systems can include one or more heteroatoms in one or both rings.“Heteroaryl” includes ring systems wherein the heteroaryl ring, asdefined above, is fused with one or more carbocyclyl or heterocyclylgroups wherein the point of attachment is on the heteroaryl ring, and insuch instances, the number of ring members continue to designate thenumber of ring members in the heteroaryl ring system. “Heteroaryl” alsoincludes ring systems wherein the heteroaryl ring, as defined above, isfused with one or more aryl groups wherein the point of attachment iseither on the aryl or heteroaryl ring, and in such instances, the numberof ring members designates the number of ring members in the fusedpolycyclic (aryl/heteroaryl) ring system. Polycyclic heteroaryl groupswherein one ring does not contain a heteroatom (e.g., indolyl,quinolinyl, carbazolyl, and the like) the point of attachment can be oneither ring, i.e., either the ring bearing a heteroatom (e.g.,2-indolyl) or the ring that does not contain a heteroatom (e.g.,5-indolyl). A heteroaryl group be monovalent or may have more than onepoint of attachment to another moiety (e.g., it may be divalent,trivalent, etc), although the valency may be specified directly in thename of the group. For example, “triazoldiyl” refers to a divalenttriazolyl moiety.

In some embodiments, a heteroaryl group is a 5-10 membered aromatic ringsystem having ring carbon atoms and 1-4 ring heteroatoms provided in thearomatic ring system, wherein each heteroatom is independently selectedfrom nitrogen, oxygen, and sulfur (“5-10 membered heteroaryl”). In someembodiments, a heteroaryl group is a 5-8 membered aromatic ring systemhaving ring carbon atoms and 1-4 ring heteroatoms provided in thearomatic ring system, wherein each heteroatom is independently selectedfrom nitrogen, oxygen, and sulfur (“5-8 membered heteroaryl”). In someembodiments, a heteroaryl group is a 5-6 membered aromatic ring systemhaving ring carbon atoms and 1-4 ring heteroatoms provided in thearomatic ring system, wherein each heteroatom is independently selectedfrom nitrogen, oxygen, and sulfur (“5-6 membered heteroaryl”). In someembodiments, the 5-6 membered heteroaryl has 1-3 ring heteroatomsselected from nitrogen, oxygen, and sulfur. In some embodiments, the 5-6membered heteroaryl has 1-2 ring heteroatoms selected from nitrogen,oxygen, and sulfur. In some embodiments, the 5-6 membered heteroaryl has1 ring heteroatom selected from nitrogen, oxygen, and sulfur. Unlessotherwise specified, each instance of a heteroaryl group isindependently unsubstituted (an “unsubstituted heteroaryl”) orsubstituted (a “substituted heteroaryl”) with one or more substituents.

Exemplary 5-membered heteroaryl groups containing 1 heteroatom include,without limitation, pyrrolyl, furanyl, and thiophenyl. Exemplary5-membered heteroaryl groups containing 2 heteroatoms include, withoutlimitation, imidazolyl, pyrazolyl, oxazolyl, isoxazolyl, thiazolyl, andisothiazolyl. Exemplary 5-membered heteroaryl groups containing 3heteroatoms include, without limitation, triazolyl, oxadiazolyl, andthiadiazolyl. Exemplary 5-membered heteroaryl groups containing 4heteroatoms include, without limitation, tetrazolyl. Exemplary6-membered heteroaryl groups containing 1 heteroatom include, withoutlimitation, pyridinyl. Exemplary 6-membered heteroaryl groups containing2 heteroatoms include, without limitation, pyridazinyl, pyrimidinyl, andpyrazinyl. Exemplary 6-membered heteroaryl groups containing 3 or 4heteroatoms include, without limitation, triazinyl and tetrazinyl,respectively. Exemplary 7-membered heteroaryl groups containing 1heteroatom include, without limitation, azepinyl, oxepinyl, andthiepinyl. Exemplary 5,6-bicyclic heteroaryl groups include, withoutlimitation, indolyl, isoindolyl, indazolyl, benzotriazolyl,benzothiophenyl, isobenzothiophenyl, benzofuranyl, benzoisofuranyl,benzimidazolyl, benzoxazolyl, benzisoxazolyl, benzoxadiazolyl,benzthiazolyl, benzisothiazolyl, benzthiadiazolyl, indolizinyl, andpurinyl. Exemplary 6,6-bicyclic heteroaryl groups include, withoutlimitation, naphthyridinyl, pteridinyl, quinolinyl, isoquinolinyl,cinnolinyl, quinoxalinyl, phthalazinyl, and quinazolinyl. Exemplarytricyclic heteroaryl groups include, without limitation,phenanthridinyl, dibenzofuranyl, carbazolyl, acridinyl, phenothiazinyl,phenoxazinyl and phenazinyl.

As understood from the above, alkyl, alkenyl, alkynyl, carbocyclyl,aryl, and heteroaryl groups are, in certain embodiments, optionallysubstituted. Optionally substituted refers to a group which may besubstituted or unsubstituted (e.g., “substituted” or “unsubstituted”alkyl). In general, the term “substituted” means that at least onehydrogen present on a group is replaced with a permissible substituent,e.g., a substituent which upon substitution results in a stablecompound, e.g., a compound which does not spontaneously undergotransformation such as by rearrangement, cyclization, elimination, orother reaction. Unless otherwise indicated, a “substituted” group has asubstituent at one or more substitutable positions of the group, andwhen more than one position in any given structure is substituted, thesubstituent is either the same or different at each position. The term“substituted” is contemplated to include substitution with allpermissible substituents of organic compounds, any of the substituentsdescribed herein that results in the formation of a stable compound. Thepresent disclosure contemplates any and all such combinations in orderto arrive at a stable compound. For purposes of this disclosure,heteroatoms such as nitrogen may have hydrogen substituents and/or anysuitable substituent as described herein which satisfy the valencies ofthe heteroatoms and results in the formation of a stable moiety.

Affixing the suffix “ene” to a group indicates the group is a polyvalent(e.g., bivalent, trivalent, tetravalent, or pentavalent) moiety. Incertain embodiments, affixing the suffix “ene” to a group indicates thegroup is a bivalent moiety.

Exemplary carbon atom substituents include, but are not limited to,halogen, —CN, —NO₂, —N₃, —SO₂H, —SO₃H, —OH, —OR^(aa), —ON(R^(bb))₂,—N(R^(bb))₂, —N(R^(bb))₃ ⁺X⁻, —N(OR^(cc))R^(bb), —SH, —SR^(aa),—SSR^(cc), —C(═O)R^(aa), —CO₂H, —CHO, —C(OR^(cc))₂, —CO₂R^(aa),—OC(═O)R^(aa), —OCO₂R^(aa), —C(═O)N(R^(bb))₂, —OC(═O)N(R^(bb))₂,—NR^(bb)C(═O)R^(aa), —NR^(bb)CO₂R^(aa), —NR^(bb)C(═O)N(R^(bb))₂,—C(═NR^(bb))R^(aa), —C(═NR^(bb))OR^(aa), —OC(═NR^(bb))R^(aa),—OC(═NR^(bb))OR^(aa), —C(═NR^(bb))N(R^(bb))₂, —OC(═NR^(bb))N(R^(bb))₂,—NR^(bb)C(═NR^(bb))N(R^(bb))₂, —C(═O)NR^(bb)SO₂R^(aa),—NR^(bb)SO₂R^(aa), —SO₂N(R^(bb))₂, —SO₂R^(aa), —SO₂OR^(aa), —OSO₂R^(aa),—S(═O)R^(aa), —OS(═O)R^(aa), —Si(R^(aa))₃,—OSi(R^(aa))₃—C(═S)N(R^(bb))₂, —C(═O)SR^(aa), —C(═S)SR^(aa),—SC(═S)SR^(aa), —SC(═O)SR^(aa), —OC(═O)SR^(aa), —SC(═O)OR^(aa),—SC(═O)R^(aa), —P(═O)(R^(aa))₂, —P(═O)(OR^(cc))₂, —OP(═O)(R^(aa))₂,—OP(═O)(OR^(cc))₂, —P(═O)(N(R^(bb))₂)₂, —OP(═O)(N(R^(bb))₂)₂,—NR^(bb)P(═O)(R^(aa))₂, —NR^(bb)P(═O)(OR^(cc))₂,—NR^(bb)P(═O)(N(R^(bb))₂)₂, —P(R^(cc))₂, —P(OR^(cc))₂, —P(R^(cc))₃ ⁺X⁻,—P(OR^(cc))₃ ⁺X⁻, —P(R^(cc))₄, —P(OR^(cc))₄, —OP(R^(cc))₂, —OP(R^(cc))₃⁺X⁻, —OP(OR^(cc))₂, —OP(OR^(cc))₃ ⁺X⁻, —OP(R^(cc))₄, —OP(OR^(cc))₄,—B(R^(aa))₂, —B(OR^(cc))₂, —BR^(aa)(OR^(cc)), C₁₋₁₀ alkyl, C₁₋₁₀perhaloalkyl, C₂₋₁₀ alkenyl, C₂₋₁₀ alkynyl, heteroC₁₋₁₀ alkyl,heteroC₂₋₁₀ alkenyl, heteroC₂₋₁₀ alkynyl, C₃₋₁₀ carbocyclyl, 3-14membered heterocyclyl, C₆₋₁₄ aryl, and 5-14 membered heteroaryl, whereineach alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl,carbocyclyl, heterocyclyl, aryl, and heteroaryl is independentlysubstituted with 0, 1, 2, 3, 4, or 5 R^(dd) groups; wherein X⁻ is acounterion;

or two geminal hydrogens on a carbon atom are replaced with the group═O, ═S, ═NN(R^(bb))₂, ═NNR^(bb)C(═O)R^(aa), ═NNR^(bb)C(═O)OR^(aa),═NNR^(bb)S(═O)₂R^(aa), ═NR^(bb), or ═NOR^(cc);

each instance of R^(aa) is, independently, selected from C₁₋₁₀ alkyl,C₁₋₁₀ perhaloalkyl, C₂₋₁₀ alkenyl, C₂₋₁₀ alkynyl, heteroC₁₋₁₀ alkyl,heteroC₂₋₁₀alkenyl, heteroC₂₋₁₀alkynyl, C₃₋₁₀ carbocyclyl, 3-14 memberedheterocyclyl, C₆₋₁₄ aryl, and 5-14 membered heteroaryl, or two R^(aa)groups are joined to form a 3-14 membered heterocyclyl or 5-14 memberedheteroaryl ring, wherein each alkyl, alkenyl, alkynyl, heteroalkyl,heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl, aryl, andheteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5 R^(dd)groups;

each instance of R^(bb) is, independently, selected from hydrogen, —OH,—OR^(aa), —N(R^(cc))₂, —CN, —C(═O)R^(aa), —C(═O)N(R^(cc))₂, —CO₂R^(aa),—SO₂R^(aa), —C(═NR^(cc))OR^(aa), —C(═NR^(cc))N(R^(cc))₂, —SO₂N(R^(cc))₂,—SO₂R^(cc), —SO₂OR^(cc), —SOR^(aa), —C(═S)N(R^(cc))₂, —C(═O)SR^(cc),—C(═S)SR^(cc), —P(═O)(R^(aa))₂, —P(═O)(OR^(cc))₂, —P(═O)(N(R^(cc))₂)₂,C₁₋₁₀ alkyl, C₁₋₁₀ perhaloalkyl, C₂₋₁₀ alkenyl, C₂₋₁₀ alkynyl,heteroC₁₋₁₀alkyl, heteroC₂₋₁₀alkenyl, heteroC₂₋₁₀alkynyl, C₃₋₁₀carbocyclyl, 3-14 membered heterocyclyl, C₆₋₁₄ aryl, and 5-14 memberedheteroaryl, or two R^(bb) groups are joined to form a 3-14 memberedheterocyclyl or 5-14 membered heteroaryl ring, wherein each alkyl,alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl,carbocyclyl, heterocyclyl, aryl, and heteroaryl is independentlysubstituted with 0, 1, 2, 3, 4, or 5 R^(dd) groups; wherein X⁻ is acounterion;

each instance of R^(cc) is, independently, selected from hydrogen, C₁₋₁₀alkyl, C₁₋₁₀ perhaloalkyl, C₂₋₁₀ alkenyl, C₂₋₁₀ alkynyl, heteroC₁₋₁₀alkyl, heteroC₂₋₁₀ alkenyl, heteroC₂₋₁₀ alkynyl, C₃₋₁₀ carbocyclyl, 3-14membered heterocyclyl, C₆₋₁₄ aryl, and 5-14 membered heteroaryl, or twoR^(cc) groups are joined to form a 3-14 membered heterocyclyl or 5-14membered heteroaryl ring, wherein each alkyl, alkenyl, alkynyl,heteroalkyl, heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl,aryl, and heteroaryl is independently substituted with 0, 1, 2, 3, 4, or5 R^(dd) groups;

each instance of R^(dd) is, independently, selected from halogen, —CN,—NO₂, —N₃, —SO₂H, —SO₃H, —OH, —OR^(ee), —ON(R^(ff))₂, —N(R^(ff))₂,—N(R^(ff))₃ ⁺X⁻, —N(OR^(ee))R^(ff), —SH, —SR^(ee), —SSR^(ee),—C(═O)R^(ee), —CO₂H, —CO₂R^(ee), —OC(═O)R^(ee), —OCO₂R^(ee),—C(═O)N(R^(ff))₂, —OC(═O)N(R^(ff))₂, —NR^(ff)C(═O)R^(ee),—NR^(ff)CO₂R^(ee), —NR^(ff)C(═O)N(R^(ff))₂, —C(═NR^(ff))OR^(ee),—OC(═NR^(ff))R^(ee), —OC(═NR^(ff))OR^(ee), —C(═NR^(ff))N(R^(ff))₂,—OC(═NR^(ff))N(R^(ff))₂, —NR^(ff)C(═NR^(ff))N(R^(ff))₂,—NR^(ff)SO₂R^(ee), —SO₂N(R^(ff))₂, —SO₂R^(ee), —SO₂OR^(ee), —OSO₂R^(ee),—S(═O)R^(ee), —Si(R^(ee))₃, —OSi(R^(ee))₃, —C(═S)N(R^(ff))₂,—C(═O)SR^(ee), —C(═S)SR^(ee), —SC(═S)SR^(ee), —P(═O)(OR^(ee))₂,—P(═O)(R^(ee))₂, —OP(═O)(R^(ee))₂, —OP(═O)(OR^(ee))₂, C₁₋₆ alkyl, C₁₋₆perhaloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, heteroC₁₋₆alkyl,heteroC₂₋₆alkenyl, heteroC₂₋₆alkynyl, C₃₋₁₀ carbocyclyl, 3-10 memberedheterocyclyl, C₆₋₁₀ aryl, 5-10 membered heteroaryl, wherein each alkyl,alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl,carbocyclyl, heterocyclyl, aryl, and heteroaryl is independentlysubstituted with 0, 1, 2, 3, 4, or 5 R^(gg) groups, or two geminalR^(dd) substituents can be joined to form ═O or ═S; wherein X⁻ is acounterion;

each instance of R^(ee) is, independently, selected from C₁₋₆ alkyl,C₁₋₆ perhaloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, heteroC₁₋₆ alkyl,heteroC₂₋₆alkenyl, heteroC₂₋₆ alkynyl, C₃₋₁₀ carbocyclyl, C₆₋₁₀ aryl,3-10 membered heterocyclyl, and 3-10 membered heteroaryl, wherein eachalkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl,carbocyclyl, heterocyclyl, aryl, and heteroaryl is independentlysubstituted with 0, 1, 2, 3, 4, or 5 R^(gg) groups;

each instance of R^(ff) is, independently, selected from hydrogen, C₁₋₆alkyl, C₁₋₆ perhaloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, heteroC₁₋₆alkyl,heteroC₂₋₆alkenyl, heteroC₂₋₆alkynyl, C₃₋₁₀ carbocyclyl, 3-10 memberedheterocyclyl, C₆₋₁₀ aryl and 5-10 membered heteroaryl, or two R^(ff)groups are joined to form a 3-10 membered heterocyclyl or 5-10 memberedheteroaryl ring, wherein each alkyl, alkenyl, alkynyl, heteroalkyl,heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl, aryl, andheteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5 R^(gg)groups; and

each instance of R^(gg) is, independently, halogen, —CN, —NO₂, —N₃,—SO₂H, —SO₃H, —OH, —OC₁₋₆ alkyl, —ON(C₁₋₆ alkyl)₂, —N(C₁₋₆ alkyl)₂,—N(C₁₋₆ alkyl)₃ ⁺X⁻, —NH(C₁₋₆ alkyl)₂ ⁺X⁻, —NH₂(C₁₋₆ alkyl)⁺X⁻, —NH₃⁺X⁻, —N(OC₁₋₆ alkyl)(C₁₋₆ alkyl), —N(OH)(C₁₋₆ alkyl), —NH(OH), —SH,—SC₁₋₆ alkyl, —SS(C₁₋₆ alkyl), —C(═O)(C₁₋₆ alkyl), —CO₂H, —CO₂(C₁₋₆alkyl), —OC(═O)(C₁₋₆ alkyl), —OCO₂(C₁₋₆ alkyl), —C(═O)NH₂, —C(═O)N(C₁₋₆alkyl)₂, —OC(═O)NH(C₁₋₆ alkyl), —NHC(═O)(C₁₋₆ alkyl), —N(C₁₋₆alkyl)C(═O)(C₁₋₆ alkyl), —NHCO₂(C₁₋₆ alkyl), —NHC(═O)N(C₁₋₆ alkyl)₂,—NHC(═O)NH(C₁₋₆ alkyl), —NHC(═O)NH₂, —C(═NH)O(C₁₋₆ alkyl), —OC(═NH)(C₁₋₆alkyl), —OC(═NH)OC₁₋₆ alkyl, —C(═NH)N(C₁₋₆ alkyl)₂, —C(═NH)NH(C₁₋₆alkyl), —C(═NH)NH₂, —OC(═NH)N(C₁₋₆ alkyl)₂, —OC(NH)NH(C₁₋₆ alkyl),—OC(NH)NH₂, —NHC(NH)N(C₁₋₆ alkyl)₂, —NHC(═NH)NH₂, —NHSO₂(C₁₋₆ alkyl),—SO₂N(C₁₋₆ alkyl)₂, —SO₂NH(C₁₋₆ alkyl), —SO₂NH₂, —SO₂C₁₋₆ alkyl,—SO₂OC₁₋₆ alkyl, —OSO₂C₁₋₆ alkyl, —SOC₁₋₆ alkyl, —Si(C₁₋₆ alkyl)₃,—OSi(C₁₋₆ alkyl)₃ —C(═S)N(C₁₋₆ alkyl)₂, C(═S)NH(C₁₋₆ alkyl), C(═S)NH₂,—C(═O)S(C₁₋₆ alkyl), —C(═S)SC₁₋₆ alkyl, —SC(═S)SC₁₋₆ alkyl, —P(═O)(OC₁₋₆alkyl)₂, —P(═O)(C₁₋₆ alkyl)₂, —OP(═O)(C₁₋₆ alkyl)₂, —OP(═O)(OC₁₋₆alkyl)₂, C₁₋₆ alkyl, C₁₋₆ perhaloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl,heteroC₁₋₆alkyl, heteroC₂₋₆alkenyl, heteroC₂₋₆alkynyl, C₃₋₁₀carbocyclyl, C₆₋₁₀ aryl, 3-10 membered heterocyclyl, 5-10 memberedheteroaryl; or two geminal R^(gg) substituents can be joined to form ═Oor ═S; wherein X⁻ is a counterion.

In certain embodiments, the carbon atom substituents are independentlyhalogen, substituted or unsubstituted C₁₋₆ alkyl, —OR^(aa), —SR^(aa),—N(R^(bb))₂, —CN, —SCN, —NO₂, —C(═O)R^(aa), —CO₂R^(aa),—C(═O)N(R^(bb))₂, —OC(═O)R^(aa), —OCO₂R^(aa), —OC(═O)N(R^(bb))₂,—NR^(bb)C(═O)R^(aa), —NR^(bb)CO₂R^(aa), or —NR^(bb)C(═O)N(R^(bb))₂. Incertain embodiments, the carbon atom substituents are independentlyhalogen, substituted or unsubstituted C₁₋₆ alkyl, —OR^(aa), —SR^(aa),—N(R^(bb))₂, —CN, —SCN, or —NO₂.

Nitrogen atoms can be substituted or unsubstituted as valency permits,and include primary, secondary, tertiary, and quaternary nitrogen atoms.Exemplary nitrogen atom substituents include, but are not limited to,hydrogen, —OH, —OR^(aa), —N(R^(cc))₂, —CN, —C(═O)R^(aa),—C(═O)N(R^(cc))₂, —CO₂R^(aa), —SO₂R^(aa), —C(═NR^(bb))R^(aa),—C(═NR^(cc))OR^(aa), —C(═NR^(cc))N(R^(cc))₂, —SO₂N(R^(cc))₂, —SO₂R^(cc),—SO₂OR^(cc), —SOR^(aa), —C(═S)N(R^(cc))₂, —C(═O)SR^(cc), —C(═S)SR^(cc),—P(═O)(OR^(cc))₂, —P(═O)(R^(aa))₂, —P(═O)(N(R^(cc))₂)₂, C₁₋₁₀ alkyl,C₁₋₁₀ perhaloalkyl, C₂₋₁₀ alkenyl, C₂₋₁₀ alkynyl, heteroC₁₋₁₀alkyl,heteroC₂₋₁₀ alkenyl, heteroC₂₋₁₀ alkynyl, C₃₋₁₀ carbocyclyl, 3-14membered heterocyclyl, C₆₋₁₄ aryl, and 5-14 membered heteroaryl, or twoR^(cc) groups attached to an N atom are joined to form a 3-14 memberedheterocyclyl or 5-14 membered heteroaryl ring, wherein each alkyl,alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl,carbocyclyl, heterocyclyl, aryl, and heteroaryl is independentlysubstituted with 0, 1, 2, 3, 4, or 5 R^(dd) groups, and wherein R^(aa),R^(bb), R^(cc) and R^(dd) are as defined above.

In certain embodiments, the substituent present on the nitrogen atom isan nitrogen protecting group (also referred to herein as an “aminoprotecting group”). Nitrogen protecting groups include, but are notlimited to, —OH, —OR^(aa), —N(R^(cc))₂, —C(═O)R^(aa), —C(═O)N(R^(cc))₂,—CO₂R^(aa), —SO₂R^(aa), —C(═NR^(cc))R^(aa), —C(═NR^(cc))OR^(aa),—C(═NR^(cc))N(R^(cc))₂, —SO₂N(R^(cc))₂, —SO₂R^(cc), —SO₂OR^(cc),—SOR^(aa), —C(═S)N(R^(cc))₂, —C(═O)SR^(cc), —C(═S)SR^(cc), C₁₋₁₀ alkyl(e.g., aralkyl, heteroaralkyl), C₂₋₁₀ alkenyl, C₂₋₁₀ alkynyl,heteroC₁₋₁₀ alkyl, heteroC₂₋₁₀ alkenyl, heteroC₂₋₁₀ alkynyl, C₃₋₁₀carbocyclyl, 3-14 membered heterocyclyl, C₆₋₁₄ aryl, and 5-14 memberedheteroaryl groups, wherein each alkyl, alkenyl, alkynyl, heteroalkyl,heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl, aralkyl, aryl,and heteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5R^(dd) groups, and wherein R^(aa), R^(bb), R^(cc) and R^(dd) are asdefined herein. Nitrogen protecting groups are well known in the art andinclude those described in detail in Protecting Groups in OrganicSynthesis, T. W. Greene and P. G. M. Wuts, 3^(rd) edition, John Wiley &Sons, 1999, incorporated herein by reference.

For example, nitrogen protecting groups such as amide groups (e.g.,—C(═O)R^(aa)) include, but are not limited to, formamide, acetamide,chloroacetamide, trichloroacetamide, trifluoroacetamide,phenylacetamide, 3-phenylpropanamide, picolinamide,3-pyridylcarboxamide, N-benzoylphenylalanyl derivative, benzamide,p-phenylbenzamide, o-nitophenylacetamide, o-nitrophenoxyacetamide,acetoacetamide, (N′-dithiobenzyloxyacylamino)acetamide,3-(p-hydroxyphenyl)propanamide, 3-(o-nitrophenyl)propanamide,2-methyl-2-(o-nitrophenoxy)propanamide,2-methyl-2-(o-phenylazophenoxy)propanamide, 4-chlorobutanamide,3-methyl-3-nitrobutanamide, o-nitrocinnamide, N-acetylmethioninederivative, o-nitrobenzamide and o-(benzoyloxymethyl)benzamide.

Nitrogen protecting groups such as carbamate groups (e.g.,—C(═O)OR^(aa)) include, but are not limited to, methyl carbamate, ethylcarbamate, 9-fluorenylmethyl carbamate (Fmoc),9-(2-sulfo)fluorenylmethyl carbamate, 9-(2,7-dibromo)fluoroenylmethylcarbamate,2,7-di-t-butyl-[9-(10,10-dioxo-10,10,10,10-tetrahydrothioxanthyl)]methylcarbamate (DBD-Tmoc), 4-methoxyphenacyl carbamate (Phenoc),2,2,2-trichloroethyl carbamate (Troc), 2-trimethylsilylethyl carbamate(Teoc), 2-phenylethyl carbamate (hZ), 1-(1-adamantyl)-1-methylethylcarbamate (Adpoc), 1,1-dimethyl-2-haloethyl carbamate,1,1-dimethyl-2,2-dibromoethyl carbamate (DB-t-BOC),1,1-dimethyl-2,2,2-trichloroethyl carbamate (TCBOC),1-methyl-1-(4-biphenylyl)ethyl carbamate (Bpoc),1-(3,5-di-t-butylphenyl)-1-methylethyl carbamate (t-Bumeoc), 2-(2′- and4′-pyridyl)ethyl carbamate (Pyoc), 2-(N,N-dicyclohexylcarboxamido)ethylcarbamate, t-butyl carbamate (BOC or Boc), 1-adamantyl carbamate (Adoc),vinyl carbamate (Voc), allyl carbamate (Alloc), 1-isopropylallylcarbamate (Ipaoc), cinnamyl carbamate (Coc), 4-nitrocinnamyl carbamate(Noc), 8-quinolyl carbamate, N-hydroxypiperidinyl carbamate, alkyldithiocarbamate, benzyl carbamate (Cbz), p-methoxybenzyl carbamate (Moz),p-nitobenzyl carbamate, p-bromobenzyl carbamate, p-chlorobenzylcarbamate, 2,4-dichlorobenzyl carbamate, 4-methylsulfinylbenzylcarbamate (Msz), 9-anthrylmethyl carbamate, diphenylmethyl carbamate,2-methylthioethyl carbamate, 2-methylsulfonylethyl carbamate,2-(p-toluenesulfonyl)ethyl carbamate, [2-(1,3-dithianyl)]methylcarbamate (Dmoc), 4-methylthiophenyl carbamate (Mtpc),2,4-dimethylthiophenyl carbamate (Bmpc), 2-phosphonioethyl carbamate(Peoc), 2-triphenylphosphonioisopropyl carbamate (Ppoc),1,1-dimethyl-2-cyanoethyl carbamate, m-chloro-p-acyloxybenzyl carbamate,p-(dihydroxyboryl)benzyl carbamate, 5-benzisoxazolylmethyl carbamate,2-(trifluoromethyl)-6-chromonylmethyl carbamate (Tcroc), m-nitrophenylcarbamate, 3,5-dimethoxybenzyl carbamate, o-nitrobenzyl carbamate,3,4-dimethoxy-6-nitrobenzyl carbamate, phenyl(o-nitrophenyl)methylcarbamate, t-amyl carbamate, S-benzyl thiocarbamate, p-cyanobenzylcarbamate, cyclobutyl carbamate, cyclohexyl carbamate, cyclopentylcarbamate, cyclopropylmethyl carbamate, p-decyloxybenzyl carbamate,2,2-dimethoxyacylvinyl carbamate, o-(N,N-dimethylcarboxamido)benzylcarbamate, 1,1-dimethyl-3-(N,N-dimethylcarboxamido)propyl carbamate,1,1-dimethylpropynyl carbamate, di(2-pyridyl)methyl carbamate,2-furanylmethyl carbamate, 2-iodoethyl carbamate, isobornyl carbamate,isobutyl carbamate, isonicotinyl carbamate,p-(p′-methoxyphenylazo)benzyl carbamate, 1-methylcyclobutyl carbamate,1-methylcyclohexyl carbamate, 1-methyl-1-cyclopropylmethyl carbamate,1-methyl-1-(3,5-dimethoxyphenyl)ethyl carbamate,1-methyl-1-(p-phenylazophenyl)ethyl carbamate, 1-methyl-1-phenylethylcarbamate, 1-methyl-1-(4-pyridyl)ethyl carbamate, phenyl carbamate,p-(phenylazo)benzyl carbamate, 2,4,6-tri-t-butylphenyl carbamate,4-(trimethylammonium)benzyl carbamate, and 2,4,6-trimethylbenzylcarbamate.

Nitrogen protecting groups such as sulfonamide groups (e.g.,—S(═O)₂R^(aa)) include, but are not limited to, p-toluenesulfonamide(Ts), benzenesulfonamide, 2,3,6-trimethyl-4-methoxybenzenesulfonamide(Mtr), 2,4,6-trimethoxybenzenesulfonamide (Mtb),2,6-dimethyl-4-methoxybenzenesulfonamide (Pme),2,3,5,6-tetramethyl-4-methoxybenzenesulfonamide (Mte),4-methoxybenzenesulfonamide (Mbs), 2,4,6-trimethylbenzenesulfonamide(Mts), 2,6-dimethoxy-4-methylbenzenesulfonamide (iMds),2,2,5,7,8-pentamethylchroman-6-sulfonamide (Pmc), methanesulfonamide(Ms), β-trimethylsilylethanesulfonamide (SES), 9-anthracenesulfonamide,4-(4′,8′-dimethoxynaphthylmethyl)benzenesulfonamide (DNMBS),benzylsulfonamide, trifluoromethylsulfonamide, and phenacylsulfonamide.

Other nitrogen protecting groups include, but are not limited to,phenothiazinyl-(10)-acyl derivative, N′-p-toluenesulfonylaminoacylderivative, N′-phenylaminothioacyl derivative, N-benzoylphenylalanylderivative, N-acetylmethionine derivative,4,5-diphenyl-3-oxazolin-2-one, N-phthalimide, N-dithiasuccinimide (Dts),N-2,3-diphenylmaleimide, N-2,5-dimethylpyrrole,N-1,1,4,4-tetramethyldisilylazacyclopentane adduct (STABASE),5-substituted 1,3-dimethyl-1,3,5-triazacyclohexan-2-one, 5-substituted1,3-dibenzyl-1,3,5-triazacyclohexan-2-one, 1-substituted3,5-dinitro-4-pyridone, N-methylamine, N-allylamine,N-[2-(trimethylsilyl)ethoxy]methylamine (SEM), N-3-acetoxypropylamine,N-(1-isopropyl-4-nitro-2-oxo-3-pyroolin-3-yl)amine, quaternary ammoniumsalts, N-benzylamine, N-di(4-methoxyphenyl)methylamine,N-5-dibenzosuberylamine, N-triphenylmethylamine (Tr),N-[(4-methoxyphenyl)diphenylmethyl]amine (MMTr),N-9-phenylfluorenylamine (PhF),N-2,7-dichloro-9-fluorenylmethyleneamine, N-ferrocenylmethylamino (Fcm),N-2-picolylamino N′-oxide, N-1,1-dimethylthiomethyleneamine,N-benzylideneamine, N-p-methoxybenzylideneamine,N-diphenylmethyleneamine, N-[(2-pyridyl)mesityl]methyleneamine,N—(N′,N′-dimethylaminomethylene)amine, N,N′-isopropylidenediamine,N-p-nitrobenzylideneamine, N-salicylideneamine,N-5-chlorosalicylideneamine,N-(5-chloro-2-hydroxyphenyl)phenylmethyleneamine,N-cyclohexylideneamine, N-(5,5-dimethyl-3-oxo-1-cyclohexenyl)amine,N-borane derivative, N-diphenylborinic acid derivative,N-[phenyl(pentaacylchromium- or tungsten)acyl]amine, N-copper chelate,N-zinc chelate, N-nitroamine, N-nitrosoamine, amine N-oxide,diphenylphosphinamide (Dpp), dimethylthiophosphinamide (Mpt),diphenylthiophosphinamide (Ppt), dialkyl phosphoramidates, dibenzylphosphoramidate, diphenyl phosphoramidate, benzenesulfenamide,o-nitrobenzenesulfenamide (Nps), 2,4-dinitrobenzenesulfenamide,pentachlorobenzenesulfenamide, 2-nitro-4-methoxybenzenesulfenamide,triphenylmethylsulfenamide, and 3-nitropyridinesulfenamide (Npys).

In certain embodiments, the substituent present on an oxygen atom is anoxygen protecting group (also referred to herein as an “hydroxylprotecting group”). Oxygen protecting groups include, but are notlimited to, —R^(aa), —N(R^(bb))₂, —C(═O)SR^(aa), —C(═O)R^(aa),—CO₂R^(aa), —C(═O)N(R^(bb))₂, —C(═NR^(bb))R^(aa), —C(═NR^(bb))OR^(aa),—C(═NR^(bb))N(R^(bb))₂, —S(═O)R^(aa), —SO₂R^(aa), —Si(R^(aa))₃,—P(R^(cc))₂, —P(R^(cc))₃ ⁺X⁻, —P(OR^(cc))₂, —P(OR^(cc))₃ ⁺X⁻,—P(═O)(R^(aa))₂, —P(═O)(OR^(cc))₂, and —P(═O)(N(R^(bb))₂)₂, wherein X⁻,R^(aa), R^(bb), and R^(cc) are as defined herein. Oxygen protectinggroups are well known in the art and include those described in detailin Protecting Groups in Organic Synthesis, T. W. Greene and P. G. M.Wuts, 3^(rd) edition, John Wiley & Sons, 1999, incorporated herein byreference.

Exemplary oxygen protecting groups include, but are not limited to,methyl, methoxylmethyl (MOM), methylthiomethyl (MTM), t-butylthiomethyl,(phenyldimethylsilyl)methoxymethyl (SMOM), benzyloxymethyl (BOM),p-methoxybenzyloxymethyl (PMBM), (4-methoxyphenoxy)methyl (p-AOM),guaiacolmethyl (GUM), t-butoxymethyl, 4-pentenyloxymethyl (POM),siloxymethyl, 2-methoxyethoxymethyl (MEM), 2,2,2-trichloroethoxymethyl,bis(2-chloroethoxy)methyl, 2-(trimethylsilyl)ethoxymethyl (SEMOR),tetrahydropyranyl (THP), 3-bromotetrahydropyranyl,tetrahydrothiopyranyl, 1-methoxycyclohexyl, 4-methoxytetrahydropyranyl(MTHP), 4-methoxytetrahydrothiopyranyl, 4-methoxytetrahydrothiopyranylS,S-dioxide, 1-[(2-chloro-4-methyl)phenyl]-4-methoxypiperidin-4-yl(CTMP), 1,4-dioxan-2-yl, tetrahydrofuranyl, tetrahydrothiofuranyl,2,3,3a,4,5,6,7,7a-octahydro-7,8,8-trimethyl-4,7-methanobenzofuran-2-yl,1-ethoxyethyl, 1-(2-chloroethoxy)ethyl, 1-methyl-1-methoxyethyl,1-methyl-1-benzyloxyethyl, 1-methyl-1-benzyloxy-2-fluoroethyl,2,2,2-trichloroethyl, 2-trimethylsilylethyl, 2-(phenylselenyl)ethyl,t-butyl, allyl, p-chlorophenyl, p-methoxyphenyl, 2,4-dinitrophenyl,benzyl (Bn), p-methoxybenzyl, 3,4-dimethoxybenzyl, o-nitrobenzyl,p-nitrobenzyl, p-halobenzyl, 2,6-dichlorobenzyl, p-cyanobenzyl,p-phenylbenzyl, 2-picolyl, 4-picolyl, 3-methyl-2-picolyl N-oxido,diphenylmethyl, p,p′-dinitrobenzhydryl, 5-dibenzosuberyl,triphenylmethyl, α-naphthyldiphenylmethyl,p-methoxyphenyldiphenylmethyl, di(p-methoxyphenyl)phenylmethyl,tri(p-methoxyphenyl)methyl, 4-(4′-bromophenacyloxyphenyl)diphenylmethyl,4,4′,4″-tris(4,5-dichlorophthalimidophenyl)methyl,4,4′,4″-tris(levulinoyloxyphenyl)methyl,4,4′,4″-tris(benzoyloxyphenyl)methyl,3-(imidazol-1-yl)bis(4′,4″-dimethoxyphenyl)methyl,1,1-bis(4-methoxyphenyl)-1′-pyrenylmethyl, 9-anthryl,9-(9-phenyl)xanthenyl, 9-(9-phenyl-10-oxo)anthryl,1,3-benzodithiolan-2-yl, benzisothiazolyl S,S-dioxido, trimethylsilyl(TMS), triethylsilyl (TES), triisopropylsilyl (TIPS),dimethylisopropylsilyl (IPDMS), diethylisopropylsilyl (DEIPS),dimethylthexylsilyl, t-butyldimethylsilyl (TBDMS), t-butyldiphenylsilyl(TBDPS), tribenzylsilyl, tri-p-xylylsilyl, triphenylsilyl,diphenylmethylsilyl (DPMS), t-butylmethoxyphenylsilyl (TBMPS), formate,benzoylformate, acetate, chloroacetate, dichloroacetate,trichloroacetate, trifluoroacetate, methoxyacetate,triphenylmethoxyacetate, phenoxyacetate, p-chlorophenoxyacetate,3-phenylpropionate, 4-oxopentanoate (levulinate),4,4-(ethylenedithio)pentanoate (levulinoyldithioacetal), pivaloate,adamantoate, crotonate, 4-methoxycrotonate, benzoate, p-phenylbenzoate,2,4,6-trimethylbenzoate (mesitoate), methyl carbonate, 9-fluorenylmethylcarbonate (Fmoc), ethyl carbonate, 2,2,2-trichloroethyl carbonate(Troc), 2-(trimethylsilyl)ethyl carbonate (TMSEC), 2-(phenylsulfonyl)ethyl carbonate (Psec), 2-(triphenylphosphonio) ethyl carbonate (Peoc),isobutyl carbonate, vinyl carbonate, allyl carbonate, t-butyl carbonate(BOC or Boc), p-nitrophenyl carbonate, benzyl carbonate, p-methoxybenzylcarbonate, 3,4-dimethoxybenzyl carbonate, o-nitrobenzyl carbonate,p-nitrobenzyl carbonate, S-benzyl thiocarbonate, 4-ethoxy-1-napththylcarbonate, methyl dithiocarbonate, 2-iodobenzoate, 4-azidobutyrate,4-nitro-4-methylpentanoate, o-(dibromomethyl)benzoate,2-formylbenzenesulfonate, 2-(methylthiomethoxy)ethyl,4-(methylthiomethoxy)butyrate, 2-(methylthiomethoxymethyl)benzoate,2,6-dichloro-4-methylphenoxyacetate,2,6-dichloro-4-(1,1,3,3-tetramethylbutyl)phenoxyacetate,2,4-bis(1,1-dimethylpropyl)phenoxyacetate, chlorodiphenylacetate,isobutyrate, monosuccinoate, (E)-2-methyl-2-butenoate,o-(methoxyacyl)benzoate, α-naphthoate, nitrate, alkylN,N,N′,N′-tetramethylphosphorodiamidate, alkyl N-phenylcarbamate,borate, dimethylphosphinothioyl, alkyl 2,4-dinitrophenylsulfenate,sulfate, methanesulfonate (mesylate), benzylsulfonate, and tosylate(Ts).

In certain embodiments, the substituent present on a sulfur atom is asulfur protecting group (also referred to as a “thiol protectinggroup”). Sulfur protecting groups include, but are not limited to,—R^(aa), —N(R^(bb))₂, —C(═O)SR^(aa), —C(═O)R^(aa), —CO₂R^(aa),—C(═O)N(R^(bb))₂, —C(═NR^(bb))R^(aa), —C(═NR^(bb))OR^(aa),—C(═NR^(bb))N(R^(bb))₂, —S(═O)R^(aa), —SO₂R^(aa), —Si(R^(aa))₃,—P(R^(cc))₂, —P(R^(cc))₃ ⁺X⁻, —P(OR^(cc))₂, —P(OR^(cc))₃ ⁺X⁻,—P(═O)(R^(aa))₂, —P(═O)(OR^(cc))₂, and —P(═O)(N(R^(bb))₂)₂, whereinR^(aa), R^(bb), and R^(cc) are as defined herein. Sulfur protectinggroups are well known in the art and include those described in detailin Protecting Groups in Organic Synthesis, T. W. Greene and P. G. M.Wuts, 3^(rd) edition, John Wiley & Sons, 1999, incorporated herein byreference.

The term “halo” or “halogen” refers to fluorine (fluoro, —F), chlorine(chloro, —Cl), bromine (bromo, —Br), or iodine (iodo, —I).

The term “hydroxyl” or “hydroxy” refers to the group —OH.

The term “thiol” or “thio” refers to the group —SH.

The term “amine” or “amino” refers to the group —NH— or —NH₂.

The term “acyl” refers to a group having the general formula—C(═O)R^(X1), —C(═O)OR^(X1), —C(═O)—O—C(═O)R^(X1), —C(═O)SR^(X1),—C(═O)N(R^(X1))₂, —C(═S)R^(X1), —C(═S)N(R^(X1))₂, and —C(═S)S(R^(X1)),—C(═NR^(X1))R^(X1), —C(═NR^(X1))OR^(X1), —C(═NR^(X1))SR^(X1), and—C(═NR^(X1))N(R^(X1))₂, wherein R^(X1) is hydrogen; halogen; substitutedor unsubstituted hydroxyl; substituted or unsubstituted thiol;substituted or unsubstituted amino; substituted or unsubstituted acyl,cyclic or acyclic, substituted or unsubstituted, branched or unbranchedaliphatic; cyclic or acyclic, substituted or unsubstituted, branched orunbranched heteroaliphatic; cyclic or acyclic, substituted orunsubstituted, branched or unbranched alkyl; cyclic or acyclic,substituted or unsubstituted, branched or unbranched alkenyl;substituted or unsubstituted alkynyl; substituted or unsubstituted aryl,substituted or unsubstituted heteroaryl, aliphaticoxy,heteroaliphaticoxy, alkyloxy, heteroalkyloxy, aryloxy, heteroaryloxy,aliphaticthioxy, heteroaliphaticthioxy, alkylthioxy, heteroalkylthioxy,arylthioxy, heteroarylthioxy, mono- or di-aliphaticamino, mono- ordi-heteroaliphaticamino, mono- or di-alkylamino, mono- ordi-heteroalkylamino, mono- or di-arylamino, or mono- ordi-heteroarylamino; or two R^(X1) groups taken together form a 5- to6-membered heterocyclic ring. Exemplary acyl groups include aldehydes(—CHO), carboxylic acids (—CO₂H), ketones, acyl halides, esters, amides,imines, carbonates, carbamates, and ureas. Acyl substituents include,but are not limited to, any of the substituents described herein, thatresult in the formation of a stable moiety (e.g., aliphatic, alkyl,alkenyl, alkynyl, heteroaliphatic, heterocyclic, aryl, heteroaryl, acyl,oxo, imino, thiooxo, cyano, isocyano, amino, azido, nitro, hydroxyl,thiol, halo, aliphaticamino, heteroaliphaticamino, alkylamino,heteroalkylamino, arylamino, heteroarylamino, alkylaryl, arylalkyl,aliphaticoxy, heteroaliphaticoxy, alkyloxy, heteroalkyloxy, aryloxy,heteroaryloxy, aliphaticthioxy, heteroaliphaticthioxy, alkylthioxy,heteroalkylthioxy, arylthioxy, heteroarylthioxy, acyloxy, and the like,each of which may or may not be further substituted).

The term “polymer” refers to a molecule including two or more (e.g., 3or more, 4 or more, 5 or more, 10 or more) repeating units which arecovalently bound together. In certain embodiments, a polymer comprises 3or more, 5 or more, 10 or more, 20 or more, 50 or more, 100 or more, 500or more, 1000 or more, 2000 or more, 3000 or more, 4000 or more, 5000 ormore, 6000 or more, 7000 or more, 8000 or more, 9000 or more, or 10000or more repeating units. In certain embodiments, a polymer comprisesmore than 5000 repeating units. The repeating units of a polymer arereferred to as “monomers.” A “homopolymer” is a polymer that consists ofa single repeating monomer. A “copolymer” is a polymer that comprisestwo or more different monomer subunits. Copolymers include, but are notlimited to, random, block, alternating, segmented, linear, branched,grafted, and tapered copolymers. A “graft polymer” is a segmentedcopolymer with a linear backbone of one composite and randomlydistributed branches of another composite. The major difference betweengraft polymers and bottlebrush polymers (or brush-arm polymers) is thegrafting density. The targeted graft density for bottlebrush polymers isthat in at least one segment of the copolymer is one graft from eachbackbone monomer unit. A “star polymer” is a polymer that consists ofseveral polymers chains connected at a core atom, core molecule, or corepolymer. Polymers may be natural (such as biopolymers like naturallyoccurring polypeptides), or synthetic (e.g., non-naturally occurring). Apolymer may have an overall molecular weight of 500 g/mol or greater, 1Da or greater, 5 Da or greater, 10 Da or greater, 50 Da or greater, 100Da or greater, 500 Da or greater, 1000 Da or greater, 2000 Da orgreater, 5000 Da or greater, 10000 Da or greater, 20000 Da or greater,or 50000 Da or greater.

The terms “living polymer” and “living polymerization” refer apolymerization where the ability of a growing polymer chain to terminatehas been removed. Chain termination and chain transfer reactions areabsent, and the rate of the chain initiation is also much larger thanthe rate of chain propagation.

The term “average molecular weight” may encompass the number averagemolecular weight (M_(n)), weight average molecular weight (M_(w)),higher average molecular weight (M_(z) or M_(z)+1), GPC/SEC (gelpermeation chromatography/size-exclusion chromatography)-determinedaverage molecular weight (M_(p)), and viscosity average molecular weight(M_(v)).

The terms “number average molecular weight,” “number average molarmass,” and “M_(n)” are measurements of the molecular mass of a polymer.The number average molecular mass is the ordinary arithmetic mean oraverage of the molecular masses of the individual polymers. It isdetermined by measuring the molecular mass of n polymer molecules,summing the masses, and dividing by n. For example, a polymer having 100repeating units of a monomer with a molecular weight of 100 g/mol wouldhave a number average molecular weight (M_(n)) of 10,000 g/mol[M_(n)=(100)*(100 g/mol)/(1)=10,000 g/mol)]. The number averagemolecular mass of a polymer can be determined by gel permeationchromatography, viscometry via the Mark-Houwink equation, colligativemethods such as vapor pressure osmometry, end-group determination, or ¹HNMR (nuclear magnetic resonance).

The term “monomer” refers to a molecule that may be covalently joined toother monomers to form a polymer. The process by which the monomers arecombined to form a polymer is called polymerization. A macromoleculewith a reactive moiety that enables it to act as a monomer is called amacromonomer. Molecules made of a small number of monomer units (up to afew dozen) are called oligomers.

The term “average hydrodynamic diameter” (D_(H)) as used herein refersto the average size of a polymer or particle. The average hydrodynamicdiameter may or may not encompass the solvation layers of polymer orparticle, and may be determined through a number of methods includingdynamic light scattering, electron microscopy (e.g., scanning electronmicroscopy, transmission electron microscopy), atomic force microscopy,and X-ray diffraction.

The term “average polydispersity” (PDI) as used herein refers to ameasure of the distribution of molecular size in a mixture, e.g., asdetermined by a chromatographic method, such as gel permeationchromatography or size exclusion chromatography, or through dynamiclight scattering.

As used herein, the term “polyethylene glycol” or “PEG” refers to anethylene glycol polymer that contains about 20 to about 2,000,000 linkedmonomers, typically about 50-1,000 linked monomers, usually about100-300. Polyethylene glycols include ethylene glycol polymer containingvarious numbers of linked monomers, e.g., PEG20, PEG30, PEG40, PEG60,PEG80, PEG100, PEG115, PEG200, PEG300, PEG400, PEG500, PEG600, PEG1000,PEG1500, PEG2000, PEG3350, PEG4000, PEG4600, PEG5000, PEG6000, PEG8000,PEG11000, PEG12000, PEG2000000, and any mixtures thereof.

The term “salt” refers to ionic compounds that result from theneutralization reaction of an acid and a base. A salt is composed of oneor more cations (positively charged ions) and one or more anions(negative ions) so that the salt is electrically neutral (without a netcharge). Salts of the compounds of this disclosure include those derivedfrom inorganic and organic acids and bases. Examples of acid additionsalts are salts of an amino group formed with inorganic acids such ashydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid, andperchloric acid, or with organic acids such as acetic acid, oxalic acid,maleic acid, tartaric acid, citric acid, succinic acid, or malonic acidor by using other methods known in the art such as ion exchange. Othersalts include adipate, alginate, ascorbate, aspartate, benzenesulfonate,benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate,citrate, cyclopentanepropionate, digluconate, dodecylsulfate,ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate,gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide,2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, laurylsulfate, malate, maleate, malonate, methanesulfonate,2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate,pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate,pivalate, propionate, stearate, succinate, sulfate, tartrate,thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and thelike. Salts derived from appropriate bases include alkali metal,alkaline earth metal, ammonium and N⁺(C₁₋₄ alkyl)₄ salts. Representativealkali or alkaline earth metal salts include sodium, lithium, potassium,calcium, magnesium, and the like. Further salts include ammonium,quaternary ammonium, and amine cations formed using counterions such ashalide, hydroxide, carboxylate, sulfate, phosphate, nitrate, lower alkylsulfonate, and aryl sulfonate.

The term “pharmaceutically acceptable salt” refers to those salts whichare, within the scope of sound medical judgment, suitable for use incontact with the tissues of humans and lower animals without unduetoxicity, irritation, allergic response, and the like, and arecommensurate with a reasonable benefit/risk ratio. Pharmaceuticallyacceptable salts are well known in the art. For example, Berge et al.describe pharmaceutically acceptable salts in detail in J.Pharmaceutical Sciences, 1977, 66, 1-19, incorporated herein byreference. Pharmaceutically acceptable salts of the compounds of thisdisclosure include those derived from suitable inorganic and organicacids and bases. Examples of pharmaceutically acceptable, nontoxic acidaddition salts are salts of an amino group formed with inorganic acids,such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuricacid, and perchloric acid or with organic acids, such as acetic acid,oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid, ormalonic acid or by using other methods known in the art such as ionexchange. Other pharmaceutically acceptable salts include adipate,alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate,borate, butyrate, camphorate, camphorsulfonate, citrate,cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate,formate, fumarate, glucoheptonate, glycerophosphate, gluconate,hemisulfate, heptanoate, hexanoate, hydroiodide,2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, laurylsulfate, malate, maleate, malonate, methanesulfonate,2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate,pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate,pivalate, propionate, stearate, succinate, sulfate, tartrate,thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and thelike. Salts derived from appropriate bases include alkali metal,alkaline earth metal, ammonium, and N⁺(C₁₋₄ alkyl)₄ ⁻ salts.Representative alkali or alkaline earth metal salts include sodium,lithium, potassium, calcium, magnesium, and the like. Furtherpharmaceutically acceptable salts include, when appropriate, nontoxicammonium, quaternary ammonium, and amine cations formed usingcounterions such as halide, hydroxide, carboxylate, sulfate, phosphate,nitrate, lower alkyl sulfonate, and aryl sulfonate.

The term “leaving group” is given its ordinary meaning in the art ofsynthetic organic chemistry and refers to an atom or a group capable ofbeing displaced by a nucleophile. Examples of suitable leaving groupsinclude halogen (such as F, Cl, Br, or I (iodine)), alkoxycarbonyloxy,aryloxycarbonyloxy, alkanesulfonyloxy, arenesulfonyloxy,alkyl-carbonyloxy (e.g., acetoxy), arylcarbonyloxy, aryloxy, methoxy,N,O-dimethylhydroxylamino, pixyl, and haloformates. In some cases, theleaving group is a sulfonic acid ester, such as toluenesulfonate(tosylate, —OTs), methanesulfonate (mesylate, —OMs),p-bromobenzenesulfonyloxy (brosylate, —OBs), —OS(═O)₂(CF₂)₃CF₃(nonaflate, —ONf), or trifluoromethanesulfonate (triflate, —OTf). Insome cases, the leaving group is a brosylate, such asp-bromobenzenesulfonyloxy. In some cases, the leaving group is anosylate, such as 2-nitrobenzenesulfonyloxy. In some embodiments, theleaving group is a sulfonate-containing group. In some embodiments, theleaving group is a tosylate group. The leaving group may also be aphosphineoxide (e.g., formed during a Mitsunobu reaction) or an internalleaving group such as an epoxide or cyclic sulfate. Other examples ofleaving groups are water, ammonia, alcohols, ether moieties, thioethermoieties, zinc halides, magnesium moieties, diazonium salts, and coppermoieties.

The term “solvent” refers to a substance that dissolves one or moresolutes, resulting in a solution. A solvent may serve as a medium forany reaction or transformation described herein. The solvent maydissolve one or more reactants or reagents in a reaction mixture. Thesolvent may facilitate the mixing of one or more reagents or reactantsin a reaction mixture. The solvent may also serve to increase ordecrease the rate of a reaction relative to the reaction in a differentsolvent. Solvents can be polar or non-polar, protic or aprotic. Commonsolvents useful in the methods described herein include, but are notlimited to, acetone, acetonitrile, benzene, benzonitrile, 1-butanol,2-butanone, butyl acetate, tert-butyl methyl ether, carbon disulfidecarbon tetrachloride, chlorobenzene, 1-chlorobutane, chloroform,cyclohexane, cyclopentane, 1,2-dichlorobenzene, 1,2-dichloroethane,dichloromethane (DCM), N,N-dimethylacetamide N,N-dimethylformamide(DMF), 1,3-dimethyl-3,4,5,6-tetrahydro-2-pyrimidinone (DMPU),1,4-dioxane, 1,3-dioxane, diethylether, 2-ethoxyethyl ether, ethylacetate, ethyl alcohol, ethylene glycol, dimethyl ether, heptane,n-hexane, hexanes, hexamethylphosphoramide (HMPA), 2-methoxyethanol,2-methoxyethyl acetate, methyl alcohol, 2-methylbutane,4-methyl-2-pentanone, 2-methyl-1-propanol, 2-methyl-2-propanol,1-methyl-2-pyrrolidinone, dimethylsulfoxide (DMSO), nitromethane,1-octanol, pentane, 3-pentanone, 1-propanol, 2-propanol, pyridine,tetrachloroethylene, tetrahyrdofuran (THF), 2-methyltetrahydrofuran,toluene, trichlorobenzene, 1,1,2-trichlorotrifluoroethane,2,2,4-trimethylpentane, trimethylamine, triethylamine,N,N-diisopropylethylamine, diisopropylamine, water, o-xylene, andp-xylene.

As used herein, the term “agent” means a molecule, group of molecules,complex or substance administered to an organism for diagnostic,therapeutic, preventative medical, or veterinary purposes. In certainembodiments, the agent is a pharmaceutical agent (e.g., a therapeuticagent, a diagnostic agent, or a prophylactic agent). In certainembodiments, the compositions disclosed herein comprise an agent(s),e.g., a first therapeutic agent (e.g., at least one (including, e.g., atleast two, at least three). In some embodiments, the compositions (e.g.,macromonomers, conjugates, or particles) can further comprise a secondtherapeutic agent, a targeting moiety, a diagnostic moiety as describedherein.

As used herein, the term “therapeutic agent” includes an agent that iscapable of providing a local or systemic biological, physiological, ortherapeutic effect in the biological system to which it is applied. Forexample, a therapeutic agent can act to control tumor growth, controlinfection or inflammation, act as an analgesic, promote anti-cellattachment, and enhance bone growth, among other functions. Othersuitable therapeutic agents can include anti-viral agents, hormones,antibodies, or therapeutic proteins. Other therapeutic agents includeprodrugs, which are agents that are not biologically active whenadministered but, upon administration to a subject are converted intobiologically active agents through metabolism or some other mechanism.

An agent (e.g., a therapeutic agent) can include a wide variety ofdifferent compounds, including chemical compounds and mixtures ofchemical compounds (e.g., small organic or inorganic molecules) such asdrug compounds (e.g., compounds approved for human or veterinary use bythe U.S. Food and Drug Administration as provided in the Code of FederalRegulations (CFR)); targeting agents; isotopically labeled chemicalcompounds; carbohydrates; saccharines; monosaccharides;oligosaccharides; polysaccharides; biological macromolecules (e.g.,peptides, proteins, and peptide analogs and derivatives);peptidomimetics; antibodies and antigen binding fragments thereof;nucleic acids (e.g., DNA or RNA); nucleotides; nucleosides;oligonucleotides; antisense oligonucleotides; polynucleotides; nucleicacid analogs and derivatives; nucleoproteins; mucoproteins;lipoproteins; synthetic polypeptides or proteins; small molecules linkedto proteins; glycoproteins; steroids; lipids; hormones; vitamins;vaccines; immunological agents; an extract made from biologicalmaterials such as bacteria, plants, fungi, or animal cells; animaltissues; naturally occurring or synthetic compositions; and anycombinations thereof.

In some embodiments, the agent is in the form of a prodrug. The term“prodrug” refer to a compound that becomes active, e.g., by solvolysis,reduction, oxidation, or under physiological conditions, to provide apharmaceutically active compound, e.g., in vivo. A prodrug can include aderivative of a pharmaceutically active compound, such as, for example,to form an ester by reaction of the acid, or acid anhydride, or mixedanhydrides moieties of the prodrug moiety with the hydroxyl moiety ofthe pharmaceutical active compound, or to form an amide prepared by theacid, or acid anhydride, or mixed anhydrides moieties of the prodrugmoiety with a substituted or unsubstituted amine of the pharmaceuticallyactive compound. Simple aliphatic or aromatic esters, amides, andanhydrides derived from acidic groups may comprise prodrugs. In someembodiments, the conjugate or particle described herein incorporates onetherapeutic agent or prodrug thereof. In some embodiments, the conjugateor particle described herein incorporates more than one therapeuticagents or prodrugs.

In some embodiments, the agent (e.g., a therapeutic agent) is a smallmolecule. As used herein, the term “small molecule” can refer tocompounds that are “natural product-like.” However, the term “smallmolecule” is not limited to “natural product-like” compounds. Rather, asmall molecule is typically characterized in that it contains severalcarbon-carbon bonds, and has a molecular weight of less than 5000Daltons (5 kDa), preferably less than 3 kDa, still more preferably lessthan 2 kDa, and most preferably less than 1 kDa. In some cases it ispreferred that a small molecule have a molecular weight equal to or lessthan 700 Daltons.

Exemplary agents (e.g., a therapeutic agents) in the compositionsinclude, but are not limited to, those found in Harrison's Principles ofInternal Medicine, 13th Edition, Eds. T. R. Harrison et al. McGraw-HillN.Y., NY; Physicians' Desk Reference, 50th Edition, 1997, Oradell N.J.,Medical Economics Co.; Pharmacological Basis of Therapeutics, 8thEdition, Goodman and Gilman, 1990; United States Pharmacopeia, TheNational Formulary, USP XII NF XVII, 1990; current edition of Goodmanand Gilman's The Pharmacological Basis of Therapeutics; and currentedition of The Merck Index, the complete contents of all of which areincorporated herein by reference.

Agents, e.g., therapeutic agents, include the herein disclosedcategories and specific examples. It is not intended that the categorybe limited by the specific examples. Those of ordinary skill in the artwill recognize also numerous other compounds that fall within thecategories and that are useful according to the present disclosure.

Examples of therapeutic agents include, but are not limited to,antimicrobial agents, analgesics, antiinflammatory agents,counterirritants, coagulation modifying agents, diuretics,sympathomimetics, anorexics, antacids and other gastrointestinal agents;antiparasitics, antidepressants, anti-hypertensives, anticholinergics,stimulants, antihormones, central and respiratory stimulants, drugantagonists, lipid-regulating agents, uricosurics, cardiac glycosides,electrolytes, ergot and derivatives thereof, expectorants, hypnotics andsedatives, antidiabetic agents, dopaminergic agents, antiemetics, musclerelaxants, para-sympathomimetics, anticonvulsants, antihistamines,beta-blockers, purgatives, antiarrhythmics, contrast materials,radiopharmaceuticals, antiallergic agents, tranquilizers, vasodilators,antiviral agents, and antineoplastic or cytostatic agents or otheragents with anti-cancer properties, or a combination thereof. Othersuitable therapeutic agents include contraceptives and vitamins as wellas micro- and macronutrients. Still other examples includeantiinfectives such as antibiotics and antiviral agents; analgesics andanalgesic combinations; anorexics; antiheimintics; antiarthritics;antiasthmatic agents; anticonvulsants; antidepressants; antidiureticagents; antidiarrleals; antihistamines; antiinflammatory agents;antimigraine preparations; antinauseants; antineoplastics;antiparkinsonism drugs; antipruritics; antipsychotics; antipyretics,antispasmodics; anticholinergics; sympathomimetics; xanthinederivatives; cardiovascular preparations including calcium channelblockers and beta-blockers such as pindolol and antiarrhythmics;anti-hypertensives; diuretics; vasodilators including general coronary,peripheral and cerebral; central nervous system stimulants; cough andcold preparations, including decongestants; hormones such as estradioland other steroids, including corticosteroids; hypnotics;immunosuppressives; muscle relaxants; parasympatholytics;psychostimulants; sedatives; and tranquilizers; and naturally derived orgenetically engineered proteins, polysaccharides, glycoproteins, orlipoproteins.

In certain instances, the diagnostic agent is an imaging agent orcontrast agent. The terms “imaging agent” and “contrast agent” refer toa substance used to enhance the contrast of structures or fluids withinthe body in medical imaging. It is commonly used to enhance thevisibility of blood vessels and the gastrointestinal tract in medicalimaging.

The terms “composition” and “formulation” are used interchangeably.

A “subject” to which administration is contemplated refers to a human(i.e., male or female of any age group, e.g., pediatric subject (e.g.,infant, child, or adolescent) or adult subject (e.g., young adult,middle-aged adult, or senior adult)) or non-human animal. In certainembodiments, the non-human animal is a mammal (e.g., primate (e.g.,cynomolgus monkey or rhesus monkey), commercially relevant mammal (e.g.,cattle, pig, horse, sheep, goat, cat, or dog), or bird (e.g.,commercially relevant bird, such as chicken, duck, goose, or turkey)).In certain embodiments, the non-human animal is a fish, reptile, oramphibian. The non-human animal may be a male or female at any stage ofdevelopment. The non-human animal may be a transgenic animal orgenetically engineered animal.

The term “administer,” “administering,” or “administration” refers toimplanting, absorbing, ingesting, injecting, inhaling, or otherwiseintroducing a compound described herein, or a composition thereof, in oron a subject.

The terms “treatment,” “treat,” and “treating” refer to reversing,alleviating, delaying the onset of, or inhibiting the progress of adisease described herein. In some embodiments, treatment may beadministered after one or more signs or symptoms of the disease havedeveloped or have been observed. In other embodiments, treatment may beadministered in the absence of signs or symptoms of the disease. Forexample, treatment may be administered to a susceptible subject prior tothe onset of symptoms (e.g., in light of a history of symptoms and/or inlight of exposure to a pathogen). Treatment may also be continued aftersymptoms have resolved, for example, to delay and/or prevent recurrence.

The term “prevent,” “preventing,” or “prevention” refers to aprophylactic treatment of a subject who is not and was not with adisease but is at risk of developing the disease or who was with adisease, is not with the disease, but is at risk of regression of thedisease. In certain embodiments, the subject is at a higher risk ofdeveloping the disease or at a higher risk of regression of the diseasethan an average healthy member of a population of subjects.

The terms “condition,” “disease,” and “disorder” are usedinterchangeably.

The term “genetic disease” refers to a disease caused by one or moreabnormalities in the genome of a subject, such as a disease that ispresent from birth of the subject. Genetic diseases may be heritable andmay be passed down from the parents' genes. A genetic disease may alsobe caused by mutations or changes of the DNAs and/or RNAs of thesubject. In such cases, the genetic disease will be heritable if itoccurs in the germline. Exemplary genetic diseases include, but are notlimited to, Aarskog-Scott syndrome, Aase syndrome, achondroplasia,acrodysostosis, addiction, adreno-leukodystrophy, albinism,ablepharon-macrostomia syndrome, alagille syndrome, alkaptonuria,alpha-1 antitrypsin deficiency, Alport's syndrome, Alzheimer's disease,asthma, autoimmune polyglandular syndrome, androgen insensitivitysyndrome, Angelman syndrome, ataxia, ataxia telangiectasia,atherosclerosis, attention deficit hyperactivity disorder (ADHD),autism, baldness, Batten disease, Beckwith-Wiedemann syndrome, Bestdisease, bipolar disorder, brachydactyl), breast cancer, Burkittlymphoma, chronic myeloid leukemia, Charcot-Marie-Tooth disease, Crohn'sdisease, cleft lip, Cockayne syndrome, Coffin Lowry syndrome, coloncancer, congenital adrenal hyperplasia, Cornelia de Lange syndrome,Costello syndrome, Cowden syndrome, craniofrontonasal dysplasia,Crigler-Najjar syndrome, Creutzfeldt-Jakob disease, cystic fibrosis,deafness, depression, diabetes, diastrophic dysplasia, DiGeorgesyndrome, Down's syndrome, dyslexia, Duchenne muscular dystrophy,Dubowitz syndrome, ectodermal dysplasia Ellis-van Creveld syndrome,Ehlers-Danlos, epidermolysis bullosa, epilepsy, essential tremor,familial hypercholesterolemia, familial Mediterranean fever, fragile Xsyndrome, Friedreich's ataxia, Gaucher disease, glaucoma, glucosegalactose malabsorption, glutaricaciduria, gyrate atrophy, GoldbergShprintzen syndrome (velocardiofacial syndrome), Gorlin syndrome,Hailey-Hailey disease, hemihypertrophy, hemochromatosis, hemophilia,hereditary motor and sensory neuropathy (HMSN), hereditary non polyposiscolorectal cancer (HNPCC), Huntington's disease, immunodeficiency withhyper-IgM, juvenile onset diabetes, Klinefelter's syndrome, Kabukisyndrome, Leigh's disease, long QT syndrome, lung cancer, malignantmelanoma, manic depression, Marfan syndrome, Menkes syndrome,miscarriage, mucopolysaccharide disease, multiple endocrine neoplasia,multiple sclerosis, muscular dystrophy, myotrophic lateral sclerosis,myotonic dystrophy, neurofibromatosis, Niemann-Pick disease, Noonansyndrome, obesity, ovarian cancer, pancreatic cancer, Parkinson'sdisease, paroxysmal nocturnal hemoglobinuria, Pendred syndrome, peronealmuscular atrophy, phenylketonuria (PKU), polycystic kidney disease,Prader-Willi syndrome, primary biliary cirrhosis, prostate cancer, REARsyndrome, Refsum disease, retinitis pigmentosa, retinoblastoma, Rettsyndrome, Sanfilippo syndrome, schizophrenia, severe combinedimmunodeficiency, sickle cell anemia, spina bifida, spinal muscularatrophy, spinocerebellar atrophy, sudden adult death syndrome, Tangierdisease, Tay-Sachs disease, thrombocytopenia absent radius syndrome,Townes-Brocks syndrome, tuberous sclerosis, Turner syndrome, Ushersyndrome, von Hippel-Lindau syndrome, Waardenburg syndrome, Weaversyndrome, Werner syndrome, Williams syndrome, Wilson's disease,xeroderma piginentosum, and Zellweger syndrome.

A “proliferative disorder” refers to a condition that occurs due toabnormal growth or extension by the multiplication of cells (Walker,Cambridge Dictionary of Biology; Cambridge University Press: Cambridge,UK, 1990). A proliferative disease may be associated with: 1) thepathological proliferation of normally quiescent cells; 2) thepathological migration of cells from their normal location (e.g.,metastasis of neoplastic cells); 3) the pathological expression ofproteolytic enzymes such as the matrix metalloproteinases (e.g.,collagenases, gelatinases, and elastases); or 4) the pathologicalangiogenesis as in proliferative retinopathy and tumor metastasis.Exemplary proliferative disorders include cancers (i.e., “malignantneoplasms”), benign neoplasms, angiogenesis, inflammatory diseases, andautoimmune diseases.

The term “angiogenesis” refers to the physiological process throughwhich new blood vessels form from pre-existing vessels. Angiogenesis isdistinct from vasculogenesis, which is the de novo formation ofendothelial cells from mesoderm cell precursors. The first vessels in adeveloping embryo form through vasculogenesis, after which angiogenesisis responsible for most blood vessel growth during normal or abnormaldevelopment. Angiogenesis is a vital process in growth and development,as well as in wound healing and in the formation of granulation tissue.However, angiogenesis is also a fundamental step in the transition oftumors from a benign state to a malignant one, leading to the use ofangiogenesis inhibitors in the treatment of cancer. Angiogenesis may bechemically stimulated by angiogenic proteins, such as growth factors(e.g., VEGF). “Pathological angiogenesis” refers to abnormal (e.g.,excessive or insufficient) angiogenesis that amounts to and/or isassociated with a disease.

The terms “neoplasm” and “tumor” are used herein interchangeably andrefer to an abnormal mass of tissue wherein the growth of the masssurpasses and is not coordinated with the growth of a normal tissue. Aneoplasm or tumor may be “benign” or “malignant,” depending on thefollowing characteristics: degree of cellular differentiation (includingmorphology and functionality), rate of growth, local invasion, andmetastasis. A “benign neoplasm” is generally well differentiated, hascharacteristically slower growth than a malignant neoplasm, and remainslocalized to the site of origin. In addition, a benign neoplasm does nothave the capacity to infiltrate, invade, or metastasize to distantsites. Exemplary benign neoplasms include, but are not limited to,lipoma, chondroma, adenomas, acrochordon, senile angiomas, seborrheickeratoses, lentigos, and sebaceous hyperplasias. In some cases, certain“benign” tumors may later give rise to malignant neoplasms, which mayresult from additional genetic changes in a subpopulation of the tumor'sneoplastic cells, and these tumors are referred to as “pre-malignantneoplasms.” An exemplary pre-malignant neoplasm is a teratoma. Incontrast, a “malignant neoplasm” is generally poorly differentiated(anaplasia) and has characteristically rapid growth accompanied byprogressive infiltration, invasion, and destruction of the surroundingtissue. Furthermore, a malignant neoplasm generally has the capacity tometastasize to distant sites. The term “metastasis,” “metastatic,” or“metastasize” refers to the spread or migration of cancerous cells froma primary or original tumor to another organ or tissue and is typicallyidentifiable by the presence of a “secondary tumor” or “secondary cellmass” of the tissue type of the primary or original tumor and not ofthat of the organ or tissue in which the secondary (metastatic) tumor islocated. For example, a prostate cancer that has migrated to bone issaid to be metastasized prostate cancer and includes cancerous prostatecancer cells growing in bone tissue.

The term “cancer” refers to a class of diseases characterized by thedevelopment of abnormal cells that proliferate uncontrollably and havethe ability to infiltrate and destroy normal body tissues. See, e.g.,Stedman's Medical Dictionary, 25th ed.; Hensyl ed.; Williams & Wilkins:Philadelphia, 1990. Exemplary cancers include, but are not limited to,acoustic neuroma; adenocarcinoma; adrenal gland cancer; anal cancer;angiosarcoma (e.g., lymphangiosarcoma, lymphangioendotheliosarcoma,hemangiosarcoma); appendix cancer; benign monoclonal gammopathy; biliarycancer (e.g., cholangiocarcinoma); bladder cancer; breast cancer (e.g.,adenocarcinoma of the breast, papillary carcinoma of the breast, mammarycancer, medullary carcinoma of the breast); brain cancer (e.g.,meningioma, glioblastomas, glioma (e.g., astrocytoma,oligodendroglioma), medulloblastoma); bronchus cancer; carcinoid tumor;cervical cancer (e.g., cervical adenocarcinoma); choriocarcinoma;chordoma; craniopharyngioma; colorectal cancer (e.g., colon cancer,rectal cancer, colorectal adenocarcinoma); connective tissue cancer;epithelial carcinoma; ependymoma; endotheliosarcoma (e.g., Kaposi'ssarcoma, multiple idiopathic hemorrhagic sarcoma); endometrial cancer(e.g., uterine cancer, uterine sarcoma); esophageal cancer (e.g.,adenocarcinoma of the esophagus, Barrett's adenocarcinoma); Ewing'ssarcoma; ocular cancer (e.g., intraocular melanoma, retinoblastoma);familiar hypereosinophilia; gall bladder cancer; gastric cancer (e.g.,stomach adenocarcinoma); gastrointestinal stromal tumor (GIST); germcell cancer; head and neck cancer (e.g., head and neck squamous cellcarcinoma, oral cancer (e.g., oral squamous cell carcinoma), throatcancer (e.g., laryngeal cancer, pharyngeal cancer, nasopharyngealcancer, oropharyngeal cancer)); hematopoietic cancers (e.g., leukemiasuch as acute lymphocytic leukemia (ALL) (e.g., B-cell ALL, T-cell ALL),acute myelocytic leukemia (AML) (e.g., B-cell AML, T-cell AML), chronicmyelocytic leukemia (CML) (e.g., B-cell CML, T-cell CML), and chroniclymphocytic leukemia (CLL) (e.g., B-cell CLL, T-cell CLL)); lymphomasuch as Hodgkin lymphoma (HL) (e.g., B-cell HL, T-cell HL) andnon-Hodgkin lymphoma (NHL) (e.g., B-cell NHL such as diffuse large celllymphoma (DLCL) (e.g., diffuse large B-cell lymphoma), follicularlymphoma, chronic lymphocytic leukemia/small lymphocytic lymphoma(CLL/SLL), mantle cell lymphoma (MCL), marginal zone B-cell lymphomas(e.g., mucosa-associated lymphoid tissue (MALT) lymphomas, nodalmarginal zone B-cell lymphoma, splenic marginal zone B-cell lymphoma),primary mediastinal B-cell lymphoma, Burkitt lymphoma, lymphoplasmacyticlymphoma (i.e., Waldenström's macroglobulinemia), hairy cell leukemia(HCL), immunoblastic large cell lymphoma, precursor B-lymphoblasticlymphoma and primary central nervous system (CNS) lymphoma; and T-cellNHL such as precursor T-lymphoblastic lymphoma/leukemia, peripheralT-cell lymphoma (PTCL) (e.g., cutaneous T-cell lymphoma (CTCL) (e.g.,mycosis fungoides, Sezary syndrome), angioimmunoblastic T-cell lymphoma,extranodal natural killer T-cell lymphoma, enteropathy type T-celllymphoma, subcutaneous panniculitis-like T-cell lymphoma, and anaplasticlarge cell lymphoma); a mixture of one or more leukemia/lymphoma asdescribed above; and multiple myeloma (MM)), heavy chain disease (e.g.,alpha chain disease, gamma chain disease, mu chain disease);hemangioblastoma; hypopharynx cancer; inflammatory myofibroblastictumors; immunocytic amyloidosis; kidney cancer (e.g., nephroblastomaa.k.a. Wilms' tumor, renal cell carcinoma); liver cancer (e.g.,hepatocellular cancer (HCC), malignant hepatoma); lung cancer (e.g.,bronchogenic carcinoma, small cell lung cancer (SCLC), non-small celllung cancer (NSCLC), adenocarcinoma of the lung); leiomyosarcoma (LMS);mastocytosis (e.g., systemic mastocytosis); muscle cancer;myelodysplastic syndrome (MDS); mesothelioma; myeloproliferativedisorder (MPD) (e.g., polycythemia vera (PV), essential thrombocytosis(ET), agnogenic myeloid metaplasia (AMM) a.k.a. myelofibrosis (MF),chronic idiopathic myelofibrosis, chronic myelocytic leukemia (CML),chronic neutrophilic leukemia (CNL), hypereosinophilic syndrome (HES));neuroblastoma; neurofibroma (e.g., neurofibromatosis (NF) type 1 or type2, schwannomatosis); neuroendocrine cancer (e.g., gastroenteropancreaticneuroendoctrine tumor (GEP-NET), carcinoid tumor); osteosarcoma (e.g.,bone cancer); ovarian cancer (e.g., cystadenocarcinoma, ovarianembryonal carcinoma, ovarian adenocarcinoma); papillary adenocarcinoma;pancreatic cancer (e.g., pancreatic andenocarcinoma, intraductalpapillary mucinous neoplasm (IPMN), Islet cell tumors); penile cancer(e.g., Paget's disease of the penis and scrotum); pinealoma; primitiveneuroectodermal tumor (PNT); plasma cell neoplasia; paraneoplasticsyndromes; intraepithelial neoplasms; prostate cancer (e.g., prostateadenocarcinoma); rectal cancer; rhabdomyosarcoma; salivary gland cancer;skin cancer (e.g., squamous cell carcinoma (SCC), keratoacanthoma (KA),melanoma, basal cell carcinoma (BCC)); small bowel cancer (e.g.,appendix cancer); soft tissue sarcoma (e.g., malignant fibroushistiocytoma (MFH), liposarcoma, malignant peripheral nerve sheath tumor(MPNST), chondrosarcoma, fibrosarcoma, myxosarcoma); sebaceous glandcarcinoma; small intestine cancer; sweat gland carcinoma; synovioma;testicular cancer (e.g., seminoma, testicular embryonal carcinoma);thyroid cancer (e.g., papillary carcinoma of the thyroid, papillarythyroid carcinoma (PTC), medullary thyroid cancer); urethral cancer;vaginal cancer; and vulvar cancer (e.g., Paget's disease of the vulva).

The term “inflammatory disease” refers to a disease caused by, resultingfrom, or resulting in inflammation. The term “inflammatory disease” mayalso refer to a dysregulated inflammatory reaction that causes anexaggerated response by macrophages, granulocytes, and/or T-lymphocytesleading to abnormal tissue damage and/or cell death. An inflammatorydisease can be either an acute or chronic inflammatory condition and canresult from infections or non-infectious causes. Inflammatory diseasesinclude, without limitation, atherosclerosis, arteriosclerosis,autoimmune disorders, multiple sclerosis, systemic lupus erythematosus,polymyalgia rheumatica (PMR), gouty arthritis, degenerative arthritis,tendonitis, bursitis, psoriasis, cystic fibrosis, arthrosteitis,rheumatoid arthritis, inflammatory arthritis, Sjogren's syndrome, giantcell arteritis, progressive systemic sclerosis (scleroderma), ankylosingspondylitis, polymyositis, dermatomyositis, pemphigus, pemphigoid,diabetes (e.g., Type I), myasthenia gravis, Hashimoto's thyroiditis,Graves' disease, Goodpasture's disease, mixed connective tissue disease,sclerosing cholangitis, inflammatory bowel disease, Crohn's disease,ulcerative colitis, pernicious anemia, inflammatory dermatoses, usualinterstitial pneumonitis (UIP), asbestosis, silicosis, bronchiectasis,berylliosis, talcosis, pneumoconiosis, sarcoidosis, desquamativeinterstitial pneumonia, lymphoid interstitial pneumonia, giant cellinterstitial pneumonia, cellular interstitial pneumonia, extrinsicallergic alveolitis, Wegener's granulomatosis and related forms ofangiitis (temporal arteritis and polyarteritis nodosa), inflammatorydermatoses, hepatitis, delayed-type hypersensitivity reactions (e.g.,poison ivy dermatitis), pneumonia, respiratory tract inflammation, AdultRespiratory Distress Syndrome (ARDS), encephalitis, immediatehypersensitivity reactions, asthma, hayfever, allergies, acuteanaphylaxis, rheumatic fever, glomerulonephritis, pyelonephritis,cellulitis, cystitis, chronic cholecystitis, ischemia (ischemic injury),reperfusion injury, allograft rejection, host-versus-graft rejection,appendicitis, arteritis, blepharitis, bronchiolitis, bronchitis,cervicitis, cholangitis, chorioamnionitis, conjunctivitis,dacryoadenitis, dermatomyositis, endocarditis, endometritis, enteritis,enterocolitis, epicondylitis, epididymitis, fasciitis, fibrositis,gastritis, gastroenteritis, gingivitis, ileitis, iritis, laryngitis,myelitis, myocarditis, nephritis, omphalitis, oophoritis, orchitis,osteitis, otitis, pancreatitis, parotitis, pericarditis, pharyngitis,pleuritis, phlebitis, pneumonitis, proctitis, prostatitis, rhinitis,salpingitis, sinusitis, stomatitis, synovitis, testitis, tonsillitis,urethritis, urocystitis, uveitis, vaginitis, vasculitis, vulvitis,vulvovaginitis, angitis, chronic bronchitis, osteomyelitis, opticneuritis, temporal arteritis, transverse myelitis, necrotizingfasciitis, and necrotizing enterocolitis. An ocular inflammatory diseaseincludes, but is not limited to, post-surgical inflammation.

An “autoimmune disease” refers to a disease arising from aninappropriate immune response of the body of a subject againstsubstances and tissues normally present in the body. In other words, theimmune system mistakes some part of the body as a pathogen and attacksits own cells. This may be restricted to certain organs (e.g., inautoimmune thyroiditis) or involve a particular tissue in differentplaces (e.g., Goodpasture's disease which may affect the basementmembrane in both the lung and kidney). The treatment of autoimmunediseases is typically with immunosuppression, e.g., medications whichdecrease the immune response. Exemplary autoimmune diseases include, butare not limited to, glomerulonephritis, Goodpasture's syndrome,necrotizing vasculitis, lymphadenitis, peri-arteritis nodosa, systemiclupus erythematosis, rheumatoid arthritis, psoriatic arthritis, systemiclupus erythematosis, psoriasis, ulcerative colitis, systemic sclerosis,dermatomyositis/polymyositis, anti-phospholipid antibody syndrome,scleroderma, pemphigus vulgaris, ANCA-associated vasculitis (e.g.,Wegener's granulomatosis, microscopic polyangiitis), uveitis, Sjogren'ssyndrome, Crohn's disease, Reiter's syndrome, ankylosing spondylitis,Lyme disease, Guillain-Barré syndrome, Hashimoto's thyroiditis, andcardiomyopathy.

The term “liver disease” or “hepatic disease” refers to damage to or adisease of the liver. Non-limiting examples of liver disease includeintrahepatic cholestasis (e.g., alagille syndrome, biliary livercirrhosis), fatty liver (e.g., alcoholic fatty liver, Reye's syndrome),hepatic vein thrombosis, hepatolenticular degeneration (i.e., Wilson'sdisease), hepatomegaly, liver abscess (e.g., amebic liver abscess),liver cirrhosis (e.g., alcoholic, biliary, and experimental livercirrhosis), alcoholic liver diseases (e.g., fatty liver, hepatitis,cirrhosis), parasitic liver disease (e.g., hepatic echinococcosis,fascioliasis, amebic liver abscess), jaundice (e.g., hemolytic,hepatocellular, cholestatic jaundice), cholestasis, portal hypertension,liver enlargement, ascites, hepatitis (e.g., alcoholic hepatitis, animalhepatitis, chronic hepatitis (e.g., autoimmune, hepatitis B, hepatitisC, hepatitis D, drug induced chronic hepatitis), toxic hepatitis, viralhuman hepatitis (e.g., hepatitis A, hepatitis B, hepatitis C, hepatitisD, hepatitis E), granulomatous hepatitis, secondary biliary cirrhosis,hepatic encephalopathy, varices, primary biliary cirrhosis, primarysclerosing cholangitis, hepatocellular adenoma, hemangiomas, bilestones, liver failure (e.g., hepatic encephalopathy, acute liverfailure), angiomyolipoma, calcified liver metastases, cystic livermetastases, fibrolamellar hepatocarcinoma, hepatic adenoma, hepatoma,hepatic cysts (e.g., Simple cysts, Polycystic liver disease,hepatobiliary cystadenoma, choledochal cyst), mesenchymal tumors(mesenchymal hamartoma, infantile hemangioendothelioma, hemangioma,peliosis hepatis, lipomas, inflammatory pseudotumor), epithelial tumors(e.g., bile duct hamartoma, bile duct adenoma), focal nodularhyperplasia, nodular regenerative hyperplasia, hepatoblastoma,hepatocellular carcinoma, cholangiocarcinoma, cystadenocarcinoma, tumorsof blood vessels, angiosarcoma, Karposi's sarcoma, hemangioendothelioma,embryonal sarcoma, fibrosarcoma, leiomyosarcoma, rhabdomyosarcoma,carcinosarcoma, teratoma, carcinoid, squamous carcinoma, primarylymphoma, peliosis hepatis, erythrohepatic porphyria, hepatic porphyria(e.g., acute intermittent porphyria, porphyria cutanea tarda), andZellweger syndrome.

The term “spleen disease” refers to a disease of the spleen. Example ofspleen diseases include, but are not limited to, splenomegaly, spleencancer, asplenia, spleen trauma, idiopathic purpura, Felty's syndrome,Hodgkin's disease, and immune-mediated destruction of the spleen.

The term “lung disease” or “pulmonary disease” refers to a disease ofthe lung. Examples of lung diseases include, but are not limited to,bronchiectasis, bronchitis, bronchopulmonary dysplasia, interstitiallung disease, occupational lung disease, emphysema, cystic fibrosis,acute respiratory distress syndrome (ARDS), severe acute respiratorysyndrome (SARS), asthma (e.g., intermittent asthma, mild persistentasthma, moderate persistent asthma, severe persistent asthma), chronicbronchitis, chronic obstructive pulmonary disease (COPD), emphysema,interstitial lung disease, sarcoidosis, asbestosis, aspergilloma,aspergillosis, pneumonia (e.g., lobar pneumonia, multilobar pneumonia,bronchial pneumonia, interstitial pneumonia), pulmonary fibrosis,pulmonary tuberculosis, rheumatoid lung disease, pulmonary embolism, andlung cancer (e.g., non-small-cell lung carcinoma (e.g., adenocarcinoma,squamous-cell lung carcinoma, large-cell lung carcinoma), small-celllung carcinoma).

A “hematological disease” includes a disease which affects ahematopoietic cell or tissue. Hematological diseases include diseasesassociated with aberrant hematological content and/or function. Examplesof hematological diseases include diseases resulting from bone marrowirradiation or chemotherapy treatments for cancer, diseases such aspernicious anemia, hemorrhagic anemia, hemolytic anemia, aplasticanemia, sickle cell anemia, sideroblastic anemia, anemia associated withchronic infections such as malaria, trypanosomiasis, HTV, hepatitisvirus or other viruses, myelophthisic anemias caused by marrowdeficiencies, renal failure resulting from anemia, anemia, polycythemia,infectious mononucleosis (EVI), acute non-lymphocytic leukemia (ANLL),acute myeloid leukemia (AML), acute promyelocytic leukemia (APL), acutemyelomonocytic leukemia (AMMoL), polycythemia vera, lymphoma, acutelymphocytic leukemia (ALL), chronic lymphocytic leukemia, Wilm's tumor,Ewing's sarcoma, retinoblastoma, hemophilia, disorders associated withan increased risk of thrombosis, herpes, thalassemia, antibody-mediateddisorders such as transfusion reactions and erythroblastosis, mechanicaltrauma to red blood cells such as micro-angiopathic hemolytic anemias,thrombotic thrombocytopenic purpura and disseminated intravascularcoagulation, infections by parasites such as Plasmodium, chemicalinjuries from, e.g., lead poisoning, and hypersplenism.

The term “neurological disease” refers to any disease of the nervoussystem, including diseases that involve the central nervous system(brain, brainstem and cerebellum), the peripheral nervous system(including cranial nerves), and the autonomic nervous system (parts ofwhich are located in both central and peripheral nervous system).Neurodegenerative diseases refer to a type of neurological diseasemarked by the loss of nerve cells, including, but not limited to,Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis,tauopathies (including frontotemporal dementia), and Huntington'sdisease. Examples of neurological diseases include, but are not limitedto, headache, stupor and coma, dementia, seizure, sleep disorders,trauma, infections, neoplasms, neuro-ophthalmology, movement disorders,demyelinating diseases, spinal cord disorders, and disorders ofperipheral nerves, muscle and neuromuscular junctions. Addiction andmental illness, include, but are not limited to, bipolar disorder andschizophrenia, are also included in the definition of neurologicaldiseases. Further examples of neurological diseases include acquiredepileptiform aphasia; acute disseminated encephalomyelitis;adrenoleukodystrophy; agenesis of the corpus callosum; agnosia; Aicardisyndrome; Alexander disease; Alpers' disease; alternating hemiplegia;Alzheimer's disease; amyotrophic lateral sclerosis; anencephaly;Angelman syndrome; angiomatosis; anoxia; aphasia; apraxia; arachnoidcysts; arachnoiditis; Arnold-Chiari malformation; arteriovenousmalformation; Asperger syndrome; ataxia telangiectasia; attentiondeficit hyperactivity disorder; autism; autonomic dysfunction; backpain; Batten disease; Behcet's disease; Bell's palsy; benign essentialblepharospasm; benign focal; amyotrophy; benign intracranialhypertension; Binswanger's disease; blepharospasm; Bloch Sulzbergersyndrome; brachial plexus injury; brain abscess; bbrain injury; braintumors (including glioblastoma multiforme); spinal tumor; Brown-Sequardsyndrome; Canavan disease; carpal tunnel syndrome (CTS); causalgia;central pain syndrome; central pontine myelinolysis; cephalic disorder;cerebral aneurysm; cerebral arteriosclerosis; cerebral atrophy; cerebralgigantism; cerebral palsy; Charcot-Marie-Tooth disease;chemotherapy-induced neuropathy and neuropathic pain; Chiarimalformation; chorea; chronic inflammatory demyelinating polyneuropathy(CIDP); chronic pain; chronic regional pain syndrome; Coffin Lowrysyndrome; coma, including persistent vegetative state; congenital facialdiplegia; corticobasal degeneration; cranial arteritis;craniosynostosis; Creutzfeldt-Jakob disease; cumulative traumadisorders; Cushing's syndrome; cytomegalic inclusion body disease(CIBD); cytomegalovirus infection; dancing eyes-dancing feet syndrome;Dandy-Walker syndrome; Dawson disease; De Morsier's syndrome;Dejerine-Klumpke palsy; dementia; dermatomyositis; diabetic neuropathy;diffuse sclerosis; dysautonomia; dysgraphia; dyslexia; dystonias; earlyinfantile epileptic encephalopathy; empty sella syndrome; encephalitis;encephaloceles; encephalotrigeminal angiomatosis; epilepsy; Erb's palsy;essential tremor; Fabry's disease; Fahr's syndrome; fainting; familialspastic paralysis; febrile seizures; Fisher syndrome; Friedreich'sataxia; frontotemporal dementia and other “tauopathies”; Gaucher'sdisease; Gerstmann's syndrome; giant cell arteritis; giant cellinclusion disease; globoid cell leukodystrophy; Guillain-Barre syndrome;HTLV-1 associated myelopathy; Hallervorden-Spatz disease; head injury;headache; hemifacial spasm; hereditary spastic paraplegia; heredopathiaatactica polyneuritiformis; herpes zoster oticus; herpes zoster;Hirayama syndrome; HIV-associated dementia and neuropathy (see alsoneurological manifestations of AIDS); holoprosencephaly; Huntington'sdisease and other polyglutamine repeat diseases; hydranencephaly;hydrocephalus; hypercortisolism; hypoxia; immune-mediatedencephalomyelitis; inclusion body myositis; incontinentia pigmenti;infantile; phytanic acid storage disease; Infantile Refsum disease;infantile spasms; inflammatory myopathy; intracranial cyst; intracranialhypertension; Joubert syndrome; Kearns-Sayre syndrome; Kennedy disease;Kinsbourne syndrome; Klippel Feil syndrome; Krabbe disease;Kugelberg-Welander disease; kuru; Lafora disease; Lambert-Eatonmyasthenic syndrome; Landau-Kleffner syndrome; lateral medullary(Wallenberg) syndrome; learning disabilities; Leigh's disease;Lennox-Gastaut syndrome; Lesch-Nyhan syndrome; leukodystrophy; Lewy bodydementia; lissencephaly; locked-in syndrome; Lou Gehrig's disease (akamotor neuron disease or amyotrophic lateral sclerosis); lumbar discdisease; lyme disease-neurological sequelae; Machado-Joseph disease;macrencephaly; megalencephaly; Melkersson-Rosenthal syndrome; Menieresdisease; meningitis; Menkes disease; metachromatic leukodystrophy;microcephaly; migraine; Miller Fisher syndrome; mini-strokes;mitochondrial myopathies; Mobius syndrome; monomelic amyotrophy; motorneurone disease; moyamoya disease; mucopolysaccharidoses; multi-infarctdementia; multifocal motor neuropathy; multiple sclerosis and otherdemyelinating disorders; multiple system atrophy with posturalhypotension; muscular dystrophy; myasthenia gravis; myelinoclasticdiffuse sclerosis; myoclonic encephalopathy of infants; myoclonus;myopathy; myotonia congenital; narcolepsy; neurofibromatosis;neuroleptic malignant syndrome; neurological manifestations of AIDS;neurological sequelae of lupus; neuromyotonia; neuronal ceroidlipofuscinosis; neuronal migration disorders; Niemann-Pick disease;O'Sullivan-McLeod syndrome; occipital neuralgia; occult spinaldysraphism sequence; Ohtahara syndrome; olivopontocerebellar atrophy;opsoclonus myoclonus; optic neuritis; orthostatic hypotension; overusesyndrome; paresthesia; Parkinson's disease; paramyotonia congenita;paraneoplastic diseases; paroxysmal attacks; Parry Romberg syndrome;Pelizaeus-Merzbacher disease; periodic paralyses; peripheral neuropathy;painful neuropathy and neuropathic pain; persistent vegetative state;pervasive developmental disorders; photic sneeze reflex; phytanic acidstorage disease; Pick's disease; pinched nerve; pituitary tumors;polymyositis; porencephaly; Post-Polio syndrome; postherpetic neuralgia(PHN); postinfectious encephalomyelitis; postural hypotension;Prader-Willi syndrome; primary lateral sclerosis; prion diseases;progressive; hemifacial atrophy; progressive multifocalleukoencephalopathy; progressive sclerosing poliodystrophy; progressivesupranuclear palsy; pseudotumor cerebri; Ramsay-Hunt syndrome (Type Iand Type II); Rasmussen's Encephalitis; reflex sympathetic dystrophysyndrome; Refsum disease; repetitive motion disorders; repetitive stressinjuries; restless legs syndrome; retrovirus-associated myelopathy; Rettsyndrome; Reye's syndrome; Saint Vitus Dance; Sandhoff disease;Schilder's disease; schizencephaly; septo-optic dysplasia; shaken babysyndrome; shingles; Shy-Drager syndrome; Sjogren's syndrome; sleepapnea; Soto's syndrome; spasticity; spina bifida; spinal cord injury;spinal cord tumors; spinal muscular atrophy; stiff-person syndrome;stroke; Sturge-Weber syndrome; subacute sclerosing panencephalitis;subarachnoid hemorrhage; subcortical arteriosclerotic encephalopathy;sydenham chorea; syncope; syringomyelia; tardive dyskinesia; Tay-Sachsdisease; temporal arteritis; tethered spinal cord syndrome; Thomsendisease; thoracic outlet syndrome; tic douloureux; Todd's paralysis;Tourette syndrome; transient ischemic attack; transmissible spongiformencephalopathies; transverse myelitis; traumatic brain injury; tremor;trigeminal neuralgia; tropical spastic paraparesis; tuberous sclerosis;vascular dementia (multi-infarct dementia); vasculitis includingtemporal arteritis; Von Hippel-Lindau Disease (VHL); Wallenberg'ssyndrome; Werdnig-Hoffman disease; West syndrome; whiplash; Williamssyndrome; Wilson's disease; and Zellweger syndrome.

The term “painful condition” includes, but is not limited to,neuropathic pain (e.g., peripheral neuropathic pain), central pain,deafferentiation pain, chronic pain (e.g., chronic nociceptive pain, andother forms of chronic pain such as post-operative pain, e.g., painarising after hip, knee, or other replacement surgery), pre-operativepain, stimulus of nociceptive receptors (nociceptive pain), acute pain(e.g., phantom and transient acute pain), noninflammatory pain,inflammatory pain, pain associated with cancer, wound pain, burn pain,postoperative pain, pain associated with medical procedures, painresulting from pruritus, painful bladder syndrome, pain associated withpremenstrual dysphoric disorder and/or premenstrual syndrome, painassociated with chronic fatigue syndrome, pain associated with pre-termlabor, pain associated with withdrawal symptoms from drug addiction,joint pain, arthritic pain (e.g., pain associated with crystallinearthritis, osteoarthritis, psoriatic arthritis, gouty arthritis,reactive arthritis, rheumatoid arthritis or Reiter's arthritis),lumbosacral pain, musculo-skeletal pain, headache, migraine, muscleache, lower back pain, neck pain, toothache, dental/maxillofacial pain,visceral pain and the like. One or more of the painful conditionscontemplated herein can comprise mixtures of various types of painprovided above and herein (e.g. nociceptive pain, inflammatory pain,neuropathic pain, etc.). In some embodiments, a particular pain candominate. In other embodiments, the painful condition comprises two ormore types of pains without one dominating. A skilled clinician candetermine the dosage to achieve a therapeutically effective amount for aparticular subject based on the painful condition.

The term “psychiatric disorder” refers to a disease of the mind andincludes diseases and disorders listed in the Diagnostic and StatisticalManual of Mental Disorders—Fourth Edition (DSM-IV), published by theAmerican Psychiatric Association, Washington D. C. (1994). Psychiatricdisorders include, but are not limited to, anxiety disorders (e.g.,acute stress disorder agoraphobia, generalized anxiety disorder,obsessive-compulsive disorder, panic disorder, posttraumatic stressdisorder, separation anxiety disorder, social phobia, and specificphobia), childhood disorders, (e.g., attention-deficit/hyperactivitydisorder, conduct disorder, and oppositional defiant disorder), eatingdisorders (e.g., anorexia nervosa and bulimia nervosa), mood disorders(e.g., depression, bipolar disorder, cyclothymic disorder, dysthymicdisorder, and major depressive disorder), personality disorders (e.g.,antisocial personality disorder, avoidant personality disorder,borderline personality disorder, dependent personality disorder,histrionic personality disorder, narcissistic personality disorder,obsessive-compulsive personality disorder, paranoid personalitydisorder, schizoid personality disorder, and schizotypal personalitydisorder), psychotic disorders (e.g., brief psychotic disorder,delusional disorder, schizoaffective disorder, schizophreniformdisorder, schizophrenia, and shared psychotic disorder),substance-related disorders (e.g., alcohol dependence, amphetaminedependence, Cannabis dependence, cocaine dependence, hallucinogendependence, inhalant dependence, nicotine dependence, opioid dependence,phencyclidine dependence, and sedative dependence), adjustment disorder,autism, delirium, dementia, multi-infarct dementia, learning and memorydisorders (e.g., amnesia and age-related memory loss), and Tourette'sdisorder.

The term “metabolic disorder” refers to any disorder that involves analteration in the normal metabolism of carbohydrates, lipids, proteins,nucleic acids, or a combination thereof. A metabolic disorder isassociated with either a deficiency or excess in a metabolic pathwayresulting in an imbalance in metabolism of nucleic acids, proteins,lipids, and/or carbohydrates. Factors affecting metabolism include, andare not limited to, the endocrine (hormonal) control system (e.g., theinsulin pathway, the enteroendocrine hormones including GLP-1, PYY orthe like), the neural control system (e.g., GLP-1 in the brain), or thelike. Examples of metabolic disorders include, but are not limited to,diabetes (e.g., Type I diabetes, Type II diabetes, gestationaldiabetes), hyperglycemia, hyperinsulinemia, insulin resistance, andobesity.

An “effective amount” of a polymer or composition described hereinrefers to an amount sufficient to elicit the desired biologicalresponse. An effective amount of a polymer or composition describedherein may vary depending on such factors as the desired biologicalendpoint, the pharmacokinetics of the composition, the condition beingtreated, the mode of administration, and the age and health of thesubject. In certain embodiments, an effective amount is atherapeutically effective amount. In certain embodiments, an effectiveamount is a prophylactically effective amount. In certain embodiments,an effective amount is the amount of a composition or pharmaceuticalcomposition described herein in a single dose. In certain embodiments,an effective amount is the combined amounts of a composition orpharmaceutical composition described herein in multiple doses.

A “therapeutically effective amount” of a polymer or compositiondescribed herein is an amount sufficient to provide a therapeuticbenefit in the treatment of a condition or to delay or minimize one ormore symptoms associated with the condition. A therapeutically effectiveamount of a polymer or composition means an amount of therapeutic agent,alone or in combination with other therapies, which provides atherapeutic benefit in the treatment of the condition. The term“therapeutically effective amount” can encompass an amount that improvesoverall therapy, reduces or avoids symptoms, signs, or causes of thecondition, and/or enhances the therapeutic efficacy of anothertherapeutic agent.

A “prophylactically effective amount” of a polymer or compositiondescribed herein is an amount sufficient to prevent a condition, or oneor more symptoms associated with the condition or prevent itsrecurrence. A prophylactically effective amount of a polymer orcomposition means an amount of a therapeutic agent, alone or incombination with other agents, which provides a prophylactic benefit inthe prevention of the condition. The term “prophylactically effectiveamount” can encompass an amount that improves overall prophylaxis orenhances the prophylactic efficacy of another prophylactic agent.

The terms “nucleic acid” or “nucleic acid sequence”, “nucleic acidmolecule”, “nucleic acid fragment” or “polynucleotide” are usedinterchangeably. A polynucleotide molecule is a biopolymer composed ofnucleotide monomers covalently bonded in a chain. DNA (deoxyribonucleicacid) and RNA (ribonucleic acid) are examples of polynucleotides withdistinct biological function. DNA consists of two chains ofpolynucleotides, with each chain in the form of a helical spiral. RNA ismore often found in nature as a single-strand folded onto itself.Exemplary types of RNA include double-stranded RNA (dsRNA), smallinterfering RNA (siRNA), short hairpin (shRNA), microRNA (miRNA),messenger RNA (mRNA), antisense RNA, transfer RNA (tRNA), small nuclearRNA (snRNA), and ribosomal RNA (rRNA).

The term “mRNA” or “mRNA molecule” refers to messenger RNA, or the RNAthat serves as a template for protein synthesis in a cell. The sequenceof a strand of mRNA is based on the sequence of a complementary strandof DNA comprising a sequence coding for the protein to be synthesized.

The term “siRNA” or “siRNA molecule” refers to small inhibitory RNAduplexes that induce the RNA interference (RNAi) pathway, where thesiRNA interferes with the expression of specific genes with acomplementary nucleotide sequence. siRNA molecules can vary in length(e.g., between 18-30 or 20-25 basepairs) and contain varying degrees ofcomplementarity to their target mRNA in the antisense strand. Some siRNAhave unpaired overhanging bases on the 5′ or 3′ end of the sense strandand/or the antisense strand. The term siRNA includes duplexes of twoseparate strands, as well as single strands that can form hairpinstructures comprising a duplex region.

The term “RNA interference” or “RNAi” refers to a biological process inwhich RNA molecules inhibit gene expression or translation, byneutralizing targets mRNA molecules. Since the discovery of RNAi and itsregulatory potentials, it has become evident that RNAi has immensepotential in suppression of desired genes. RNAi is now known as precise,efficient, stable, and better than antisense technology for genesuppression. Two types of small ribonucleic acids molecules are centralto RNA interference: miRNA and siRNA. These small RNAs can bind to mRNAmolecules and either increase or decrease their activity (e.g.,preventing an mRNA from being translated into a protein). The RNAipathway is found in many eukaryotes, including animals, and is initiatedby the enzyme Dicer, which cleaves long dsRNA molecules into shortdouble-stranded fragments of ˜20 nucleotide siRNAs. Each siRNA isunwound into two single-stranded RNAs (ssRNAs), the passenger strand andthe guide strand. The passenger strand is degraded and the guide strandis incorporated into the RNA-induced silencing complex (RISC). The mostwell-studied outcome is post-transcriptional gene silencing, whichoccurs when the guide strand pairs with a complementary sequence in amRNA molecule and induces cleavage by Argonaute 2 (Ago2), the catalyticcomponent of the RISC complex. In some organisms, this process spreadssystematically, despite the initially limited molar concentrations ofsiRNA.

The term “biodegradable” or “biodegradation” refers to thedisintegration of materials by biological means. Organic material can bedegraded aerobically or anaerobically. Decomposition of biodegradablesubstances may include both biological and abiotic steps, such ashydrolysis.

The term “biocompatible” or “biocompatibility” refers to the ability ofa material to perform with an appropriate host response in a specificsituation. In particular, the terms refer to the ability of abiomaterial to perform its desired function with respect to a medicaltherapy without eliciting any undesirable local or systematic effects inthe recipient or beneficiary of that therapy, but generating the mostappropriate beneficial cellular or tissue response in that specificsituation, and optimizing the clinically relevant performance of thattherapy.

The disclosure is not intended to be limited in any manner by the aboveexemplary listing of substituents. Additional terms may be defined inother sections of this disclosure.

Detailed Description of Certain Embodiments

Before the disclosed systems, compositions, methods, reagents, and kitsare described in more detail, it should be understood that the aspectsdescribed herein are not limited to specific embodiments, methods,apparati, or configurations, and as such can, of course, vary. It isalso to be understood that the terminology used herein is for thepurpose of describing particular aspects only and, unless specificallydefined herein, is not intended to be limiting.

Described herein are novel PBAEs derived from polylactone (PCL)diacrylates. In some embodiments, the PBAEs include dipolymers derivedfrom one diacrylate monomers and one amine, terpolymers derived from onediacrylate monomer and two different amines. The PCL-derived PBAEspromote stable formulations with agents such as oligonucleotides,leading to transfection efficacy several times higher than transfectionagents such as PEI, for example, and further leading to target organspecificity. The PCL-derived PBAEs are biodegradable and biocompatible,offering higher efficacy and lower toxicity for a variety ofapplications.

Polymers

In one aspect, provided herein is a polymer of Formula (I):

or a pharmaceutically acceptable salt thereof, wherein:

is a single bond or a double bond;

A is hydrogen or —XR₃;

each B independently is a diradical of the formula:

each D independently is:

E is hydrogen or —XR₄;

each R, R′, and R″ independently is optionally substituted, cyclic oracyclic aliphatic; optionally substituted, cyclic or acyclicheteroaliphatic; or a combination thereof;

each R_(a) independently is hydrogen or optionally substitutedaliphatic;

R₁ and R₂ independently are selected from hydrogen; optionallysubstituted, cyclic or acyclic aliphatic; optionally substituted, cyclicor acyclic heteroaliphatic; optionally substituted aryl; optionallysubstituted heteroaryl; and a nitrogen protecting group; or R₁ and R₂are combined to form a ring;

R₃ and R₄ independently are selected from hydrogen; optionallysubstituted, cyclic or acyclic aliphatic; optionally substituted, cyclicor acyclic heteroaliphatic; optionally substituted aryl; optionallysubstituted heteroaryl; and a protecting group;

X is O, S, NH or NR_(X), wherein R_(X) is optionally substituted, cyclicor acyclic aliphatic; optionally substituted, cyclic or acyclicheteroaliphatic; optionally substituted aryl; or optionally substitutedheteroaryl;

X′ is O or NR_(y), wherein R_(y) is hydrogen or optionally substitutedaliphatic;

each m independently is an integer between 1 and 100, inclusive; and

n is an integer between 1 and 10,000, inclusive.

In certain embodiments, the polymer of Formula (I) is of Formula (II):

or a pharmaceutically acceptable salt thereof.

In certain embodiments, the polymer of Formula (I) is of Formula (III):

or a pharmaceutically acceptable salt thereof.

In certain embodiments, the polymer of Formula (I) is of Formula (IV):

or a pharmaceutically acceptable salt thereof.

In certain embodiments, the polymer of Formula (I) is of Formula (V):

or a pharmaceutically acceptable salt thereof.

Variable A

In certain embodiments, A is hydrogen or —XR₃, wherein X and R₃ aredefined herein. In certain particular embodiments, A is —NHR₃. Incertain particular embodiments, A is —NR_(x)R₃. In certain particularembodiments, A is hydrogen. In certain particular embodiments, A is—NH₂. In certain embodiments, A is

wherein y is 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10. In certain particularembodiments, A is

Variable B

In certain embodiments, each B independently is a diradical of theformula:

wherein R, R′, and m are as defined herein. In certain embodiments, m is1, 2, 3, 4, 5, 6, 7, 8, 9, or 10. In certain particular embodiments, mis 3. In certain particular embodiments, m is 5. In certain particularembodiments, m is 7.

In certain embodiments, B is of the formula:

wherein p and q independently are 2, 3, 4, 5 or 6. In certainembodiments, m is 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10. In certainparticular embodiments, m is 3. In certain particular embodiments, m is5. In certain particular embodiments, m is 7.In certain embodiments, B is of the formula:

In certain embodiments, m is 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10. Incertain particular embodiments, m is 3. In certain particularembodiments, m is 5. In certain particular embodiments, m is 7.

Variable D

In certain embodiments, each D independently is:

wherein R₁ and R₂ are defined herein.

When D is

the groups R₁ and/or R₂ may be covalently bonded to R″ to form one ortwo cyclic structures. In certain embodiments, R₁ and R₂ are combined toform a ring.

In certain embodiments, each D is

and independently is selected from Table 1.

TABLE 1

In certain particular embodiments, at least one D diradical is of theformula:

In certain embodiments, each D is

and independently is selected from Table 2.

TABLE 2

In certain particular embodiments, at least one D diradical is

In certain embodiments, each D diradical independently is

and is selected from the moieties in Table 1 and Table 2.

In certain embodiments, the polymer comprises only one type of Ddiradical. In certain embodiments, the polymer comprises at least twotypes of D diradical. In certain embodiments, the polymer comprises onlytwo types of D diradical. In certain embodiments, the polymer comprisesat least three types of D diradical. In certain embodiments, the polymercomprises only three types of D diradical. In certain embodiments, thepolymer comprises at least four types of D diradical. In certainembodiments, the polymer comprises only four types of D diradical.

Variable E

In certain embodiments, E is hydrogen or —XR₄, wherein X and R₄ aredefined herein. In certain particular embodiments, E is —XR₄. In certainparticular embodiments, E is hydrogen. In certain particularembodiments, E is —NH₂. In certain embodiments, E is

wherein y is 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10. In certain particularembodiments, E is

Variable R

R is divalent (i.e., a diradical). In certain embodiments, each Rindependently is optionally substituted, cyclic or acyclic aliphatic,optionally substituted, cyclic or acyclic heteroaliphatic, or acombination thereof. In certain embodiments, each R independently issubstituted acyclic aliphatic. In certain embodiments, each Rindependently is unsubstituted acyclic aliphatic. In certainembodiments, each R independently is substituted cyclic aliphatic. Incertain embodiments, each R independently is unsubstituted cyclicaliphatic. In certain embodiments, each R independently is substitutedacyclic heteroaliphatic. In certain embodiments, each R independently isunsubstituted acyclic heteroaliphatic. In certain embodiments, each Rindependently is substituted cyclic heteroaliphatic. In certainembodiments, each R independently is unsubstituted cyclicheteroaliphatic.

In certain embodiments, R is of the formula:

wherein R₅ is selected from the group consisting of alkyl, heteroalkyl,alkenyl, alkynyl, haloalkyl, alkoxy, thioalkoxy, amino, alkylamino,dialkylamino, trialkylamino, acylamino, cyano, hydroxy, halo, mercapto,nitro, carboxyaldehyde, carboxy, alkoxycarbonyl, and carboxamide,wherein said alkyl, heteroalkyl, alkenyl, and alkynyl are optionallysubstituted, cyclic or acyclic, and branched or linear. In certainembodiments, R₅ is optionally substituted C₂-C₂₀ alkyl or optionallysubstituted C₂-C₂₀ heteroalkyl, and r is 1-19.

Variable R′

R′ is divalent (i.e., a diradical). In certain embodiments, each R′independently is optionally substituted, cyclic or acyclic aliphatic,optionally substituted, cyclic or acyclic heteroaliphatic, or acombination thereof. In certain embodiments, each R′ independently issubstituted acyclic aliphatic. In certain embodiments, each R′independently is unsubstituted acyclic aliphatic. In certainembodiments, each R′ independently is substituted cyclic aliphatic. Incertain embodiments, each R′ independently is unsubstituted cyclicaliphatic. In certain embodiments, each R′ independently is substitutedacyclic heteroaliphatic. In certain embodiments, each R′ independentlyis unsubstituted acyclic heteroaliphatic. In certain embodiments, eachR′ independently is substituted cyclic heteroaliphatic. In certainembodiments, each R′ independently is unsubstituted cyclicheteroaliphatic.

In certain embodiments, R′ is of the formula:

wherein R₆ is selected from the group consisting of alkyl, heteroalkyl,alkenyl, alkynyl, haloalkyl, alkoxy, thioalkoxy, amino, alkylamino,dialkylamino, trialkylamino, acylamino, cyano, hydroxy, halo, mercapto,nitro, carboxyaldehyde, carboxy, alkoxycarbonyl, and carboxamide,wherein said alkyl, heteroalkyl, alkenyl, and alkynyl are optionallysubstituted, cyclic or acyclic, and branched or linear. In certainembodiments, R₆ is optionally substituted C₂-C₂₀ alkyl or optionallysubstituted C₂-C₂₀ heteroalkyl, and r is 1-19.

Variable R″

R″ is divalent (i.e., a diradical). In certain embodiments, each R″independently is optionally substituted, cyclic or acyclic aliphatic,optionally substituted, cyclic or acyclic heteroaliphatic, or acombination thereof. In certain embodiments, each R″ independently issubstituted acyclic aliphatic. In certain embodiments, each R″independently is unsubstituted acyclic aliphatic. In certainembodiments, each R″ independently is substituted cyclic aliphatic. Incertain embodiments, each R″ independently is unsubstituted cyclicaliphatic. In certain embodiments, each R″ independently is substitutedacyclic heteroaliphatic. In certain embodiments, each R″ independentlyis unsubstituted acyclic heteroaliphatic. In certain embodiments, eachR″ independently is substituted cyclic heteroaliphatic. In certainembodiments, each R″ independently is unsubstituted cyclicheteroaliphatic.

In certain embodiments, each R″ independently is

wherein n is an integer between 1 and 20, inclusive. In certainembodiments, each R″ independently is

wherein n is an integer between 1 and 20, inclusive. In certainembodiments, each R″ independently is

wherein n is an integer between 1 and 20, inclusive; and each mindependently is an integer between 1 and 6, inclusive.

Variable R_(a)

In certain embodiments, each R_(a) independently is hydrogen oroptionally substituted aliphatic. In certain embodiments, each R_(a) ishydrogen. In certain embodiments, each R_(a) independently issubstituted aliphatic. In certain embodiments, each R_(a) isindependently unsubstituted aliphatic. In certain embodiments, eachR_(a) is independently substituted or unsubstituted C₁₋₆ alkyl. Incertain embodiments, each R_(a) is hydrogen or unsubstituted methyl. Incertain embodiments, each R_(a) is unsubstituted methyl.

Variable R₁

In certain embodiments, each R₁ independently is hydrogen, optionallysubstituted, cyclic or acyclic aliphatic, optionally substituted, cyclicor acyclic heteroaliphatic, optionally substituted aryl, or optionallysubstituted heteroaryl, or R₁ and R₂ are combined to form a ring. Incertain embodiments, each R₁ independently is hydrogen. In certainembodiments, each R₁ independently is substituted acyclic aliphatic. Incertain embodiments, each R₁ independently is unsubstituted acyclicaliphatic. In certain embodiments, each R₁ independently is substitutedcyclic aliphatic. In certain embodiments, each R₁ independently isunsubstituted cyclic aliphatic. In certain embodiments, each R₁independently is substituted acyclic heteroaliphatic. In certainembodiments, each R₁ independently is unsubstituted acyclicheteroaliphatic. In certain embodiments, each R₁ independently issubstituted cyclic heteroaliphatic. In certain embodiments, each R₁independently is unsubstituted cyclic heteroaliphatic. In certainembodiments, each R₁ independently is substituted aryl. In certainembodiments, each R₁ independently is unsubstituted aryl. In certainembodiments, each R₁ independently is substituted heteroaryl. In certainembodiments, each R₁ independently is unsubstituted heteroaryl.

In certain embodiments, at least one R₁ is optionally substituted C₁₋₆alkyl. In certain embodiments, each R₁ is optionally substituted C₁₋₆alkyl. In certain embodiments, at least one R₁ is

In certain embodiments, each R₁ is

In certain embodiments, at least one R₁ is

In certain embodiments, each R₁ is

In certain embodiments, at least one R₁ is

In certain embodiments, each R₁ is

In certain embodiments, at least one R₁ is

In certain embodiments, each R₁ is

Variable R₂

In certain embodiments, each R₂ independently is hydrogen, optionallysubstituted, cyclic or acyclic aliphatic, optionally substituted, cyclicor acyclic heteroaliphatic, optionally substituted aryl, or optionallysubstituted heteroaryl, or R₁ and R₂ are combined to form a ring. Incertain embodiments, each R₂ independently is hydrogen. In certainembodiments, each R₂ independently is substituted acyclic aliphatic. Incertain embodiments, each R₂ independently is unsubstituted acyclicaliphatic. In certain embodiments, each R₂ independently is substitutedcyclic aliphatic. In certain embodiments, each R₂ independently isunsubstituted cyclic aliphatic. In certain embodiments, each R₂independently is substituted acyclic heteroaliphatic. In certainembodiments, each R₂ independently is unsubstituted acyclicheteroaliphatic. In certain embodiments, each R₂ independently issubstituted cyclic heteroaliphatic. In certain embodiments, each R₂independently is unsubstituted cyclic heteroaliphatic. In certainembodiments, each R₂ independently is substituted aryl. In certainembodiments, each R₂ independently is unsubstituted aryl. In certainembodiments, each R₂ independently is substituted heteroaryl. In certainembodiments, each R₂ independently is unsubstituted heteroaryl. Incertain embodiments, at least one R₂ is C₁₋₆ alkyl. In certainembodiments, each R₂ is C₁₋₆ alkyl. In certain embodiments, R₁ and R₂are combined to form a 4-, 5-, 6-, or 7-membered optionally substituted,saturated or partially-unsaturated ring.

Variables R₃ and R₄

In certain embodiments, R₃ and R₄ independently, for each occurrence,are hydrogen, optionally substituted, cyclic or acyclic aliphatic,optionally substituted, cyclic or acyclic heteroaliphatic, optionallysubstituted aryl, or optionally substituted heteroaryl. In certainembodiments, R₃ and R₄ independently, for each occurrence, are hydrogen.In certain embodiments, R₃ and R₄ independently, for each occurrence,are substituted acyclic aliphatic. In certain embodiments, R₃ and R₄independently, for each occurrence, are unsubstituted acyclic aliphatic.In certain embodiments, R₃ and R₄ independently, for each occurrence,are substituted cyclic aliphatic. In certain embodiments, R₃ and R₄independently, for each occurrence, are unsubstituted cyclic aliphatic.In certain embodiments, R₃ and R₄ independently, for each occurrence,are substituted acyclic heteroaliphatic. In certain embodiments, R₃ andR₄ independently, for each occurrence, are unsubstituted acyclicheteroaliphatic. In certain embodiments, R₃ and R₄ independently, foreach occurrence, are substituted cyclic heteroaliphatic. In certainembodiments, R₃ and R₄ independently, for each occurrence, areunsubstituted cyclic heteroaliphatic. In certain embodiments, R₃ and R₄independently, for each occurrence, are substituted aryl. In certainembodiments, R₃ and R₄ independently, for each occurrence, areunsubstituted aryl. In certain embodiments, R₃ and R₄ independently, foreach occurrence, are substituted heteroaryl. In certain embodiments, R₃and R₄ independently, for each occurrence, are unsubstituted heteroaryl.

In certain embodiments, R³ and R⁴ are the same. In certain embodiments,R³ and R⁴ are different. In certain embodiments, R³ and R⁴ are differentfrom R₁. In certain embodiments, R₃ and R₄ independently are optionallysubstituted, cyclic or acyclic aliphatic, or optionally substituted,cyclic or acyclic heteroaliphatic. In certain embodiments, R₃ and R₄independently are:

wherein: o, p, q, and r are each independently an integer between 0 and20, inclusive; each instance of V is independently —O—, —S—, —NH—,—NR_(V)—, or C(R_(V))₂, wherein each instance of R_(V) is independentlyhydrogen, halogen, hydroxyl, C₁₋₆aliphatic, C₁₋₆heteroaliphatic,C₁₋₆alkoxy, amino, C₁₋₆alkylamino, di(C₁₋₆alkyl)amino, aryl, heteroaryl,thiol, alkylthioxy, or acyl. In certain embodiments, R₃ and R₄independently are

wherein m is an integer between 1 and 20, inclusive. In certainembodiments, R₃ and R₄ independently are

wherein m is an integer between 1 and 20, inclusive. In certainembodiments, R₃ and R₄ independently are

wherein m is an integer between 1 and 20, inclusive. In certainembodiments, R₃ and R₄ independently are

wherein m is an integer between 1 and 20, inclusive. In certainembodiments, R₃ and R₄ independently are

wherein m is an integer between 1 and 20, inclusive. In certainembodiments, R₃ and R₄ independently are

wherein m is an integer between 1 and 20, inclusive. In certainembodiments, R₃ and R₄ independently are

wherein m is an integer between 1 and 20, inclusive.

In certain embodiments, R³ and R⁴ independently are selected from thegroup consisting of:

In certain embodiments, R₃ and R₄ are both

wherein y is 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10.

In certain embodiments, R₃ and R₄ are both

Variable X

In certain embodiments, each X independently is O, S, NH, or NR_(X),wherein R_(X) is optionally substituted, aliphatic; optionallysubstituted carbocyclyl; optionally substituted heteroaliphatic;optionally substituted heterocyclyl; optionally substituted aryl; oroptionally substituted heteroaryl. The compositions and polymersprovided herein, each X may be the different, or both X may be the same.In certain embodiments, each X is S. In certain embodiments, each X isNH. In certain embodiments, each X is NR_(x). In certain embodiments,each R_(x) independently is substituted acyclic aliphatic. In certainembodiments, each R_(x) independently is unsubstituted acyclicaliphatic. In certain embodiments, each R_(x) independently issubstituted cyclic aliphatic. In certain embodiments, each R_(x)independently is unsubstituted cyclic aliphatic. In certain embodiments,each R_(x) independently is substituted acyclic heteroaliphatic. Incertain embodiments, each R_(x) independently is unsubstituted acyclicheteroaliphatic. In certain embodiments, each R_(x) independently issubstituted cyclic heteroaliphatic. In certain embodiments, each R_(x)independently is unsubstituted cyclic heteroaliphatic. In certainembodiments, each R_(x) independently is C₁₋₆ alkyl.

Variable X′

In certain embodiments, each X′ independently is O or NR_(y), whereinR_(y) is hydrogen; optionally substituted, aliphatic; optionallysubstituted carbocyclyl; optionally substituted heteroaliphatic;optionally substituted heterocyclyl; optionally substituted aryl; oroptionally substituted heteroaryl. In certain embodiments, each X′ isthe same. In certain embodiments, X′ is O. In certain embodiments, X′ isNR_(y). In certain embodiments, X′ is NH. In certain embodiments, eachR_(y) independently is substituted acyclic aliphatic. In certainembodiments, each R_(y) independently is unsubstituted acyclicaliphatic. In certain embodiments, each R_(y) independently issubstituted cyclic aliphatic. In certain embodiments, each R_(y)independently is unsubstituted cyclic aliphatic. In certain embodiments,each R_(y) independently is substituted acyclic heteroaliphatic. Incertain embodiments, each R_(y) independently is unsubstituted acyclicheteroaliphatic. In certain embodiments, each R_(y) independently issubstituted cyclic heteroaliphatic. In certain embodiments, each R_(y)independently is unsubstituted cyclic heteroaliphatic.

In certain embodiments, each R_(y) independently is C₁₋₆ alkyl.

Variable m

In certain embodiments, each m independently is an integer between 1 and100, inclusive. In certain embodiments, each m independently is aninteger between 1 and 50, inclusive. In certain embodiments, each mindependently is an integer between 1 and 20, inclusive. In certainembodiments, each m independently is an integer between 1 and 10,inclusive. In certain embodiments, each m independently is an integerbetween 2 and 10, inclusive. In certain embodiments, each mindependently is an integer between 3 and 10, inclusive. In certainembodiments, each m independently is an integer between 4 and 10,inclusive. In certain embodiments, each m independently is an integerbetween 5 and 10, inclusive. In certain embodiments, each mindependently is an integer between 6 and 10, inclusive. In certainembodiments, each m independently is an integer between 7 and 10,inclusive. In certain embodiments, each m independently is an integerbetween 8 and 10, inclusive. In certain embodiments, m is 3, 5, or 7.

In certain embodiments, at least 50%, at least 60%, at least 70%, atleast 80%, at least 90%, at least 95%, or at least 99% of the polymershave the same value of m.

Variable n

In certain embodiments, n is an integer between 1 and 10,000, inclusive.In certain embodiments, n is an integer between 1,000 and 10,000,inclusive. In certain embodiments, n is an integer between 5,000 and10,000, inclusive. In certain embodiments, n is an integer between 1 and1,000, inclusive. In certain embodiments, n is an integer between 10 and500, inclusive. In certain embodiments, n is an integer between 1 and100, inclusive. In certain embodiments, n is an integer between 1 and10, inclusive.

Polymer (1)

In certain embodiments, the polymer is of the formula:

wherein:

each R₁ is independently

at least one R₁ is

and

at least one R₁ is

In certain particular embodiments, the ratio of

to

is about 4:2, for example, about 3.9 to about 1.7.

In certain embodiments, each X is as defined herein. In certainparticular embodiments, each X is —NH—.

In certain embodiments, R₃ and R₄ are as defined herein. In certainparticular embodiments, R₃ and R₄ are both

In certain embodiments, m is as defined herein. In certain particularembodiments, m is about 3-4. In certain particular embodiments, m isabout 5-6. In certain embodiments, m is about 7-8.

In certain embodiments, n is as defined herein. In certain particularembodiments, n is between 1 and 10, inclusive. In a particularembodiment, n is about 5.

In a particular embodiments, Polymer (1) is of Formula B1: m=3, n isabout 5, each X is —NH—, and R₃ and R₄ are both

In a particular embodiments, Polymer (1) is of Formula C1: m=5, n isabout 5, each X is —NH—, and R₃ and R₄ are both

In a particular embodiments, Polymer (1) is of Formula D1: m=7, n isabout 5, each X is —NH—, and R₃ and R₄ are both

In certain embodiments, Polymer (1) is selected from B1, C1, and D1.

Polymer (2)

In certain embodiments, the polymer is of the formula:

wherein:

each R₁ is independently

at least

one R₁ is

and

at least one R₁ is

In certain embodiments, the ratio of

to

is about 4:2, for example, about 3.9 to about 1.7.

In certain embodiments, each X is as defined herein. In certainparticular embodiments, each X is —NH—.

In certain embodiments, R₃ and R₄ are as defined herein. In certainparticular embodiments, R₃ and R₄ are both

In certain embodiments, m is as defined herein. In certain particularembodiments, m is about 3-4. In certain particular embodiments, m isabout 5-6. In certain embodiments, m is about 7-8.

In certain embodiments, n is as defined herein. In certain particularembodiments, n is between 1 and 10, inclusive. In a particularembodiment, n is about 5.

In a particular embodiments, Polymer (2) is of Formula B2: m=3, n isabout 5, each X is —NH—, and R₃ and R₄ are both

In a particular embodiments, Polymer (2) is of Formula C2: m=5, n isabout 5, each X is —NH—, and R₃ and R₄ are both

In a particular embodiments, Polymer (2) is of Formula D2: m=7, n isabout 5, each X is —NH—, and R₃ and R₄ are both

In certain embodiments, Polymer (2) is selected from B2, C2, and D2.

Polymer (3)

In certain embodiments, the polymer is of the formula:

wherein:

each D is independently

at least one D is

and

at least one D is

In certain embodiments, the ratio of

is about 3.9 to about 1.7.

In certain embodiments, each X is as defined herein. In certainparticular embodiments, each X is —NH—.

In certain embodiments, R₃ and R₄ are as defined herein. In certainparticular embodiments, R₃ and R₄ are both

In certain embodiments, m is as defined herein. In certain particularembodiments, m is about 3-4. In certain particular embodiments, m isabout 5-6. In certain embodiments, m is about 7-8.

In certain embodiments, n is as defined herein. In certain particularembodiments, n is between 1 and 10, inclusive. In a particularembodiment, n is about 5.

In a particular embodiments, Polymer (3) is of Formula B3: m=3, n isabout 5, each X is —NH—, and R₃ and R₄ are both

In a particular embodiments, Polymer (3) is of Formula C3: m=5, n isabout 5, each X is —NH—, and R₃ and R₄ are both

In a particular embodiments, Polymer (3) is of Formula D3: m=7, n isabout 5, each X is —NH—, and R₃ and R₄ are both

In certain embodiments, Polymer (3) is selected from B3, C₃, and D3.

Polymer (4)

In certain embodiments, the polymer is of the formula:

wherein:

each R₁ is independently

at least one R₁ is

and

at least one R₁ is

In certain embodiments, the ratio of

is about 3.9 to about 1.7.

In certain embodiments, each X is as defined herein. In certainparticular embodiments, each X is —NH—.

In certain embodiments, R₃ and R₄ are as defined herein. In certainparticular embodiments, R₃ and R₄ are both

In certain embodiments, m is as defined herein. In certain particularembodiments, m is about 3-4. In certain particular embodiments, m isabout 5-6. In certain embodiments, m is about 7-8.

In certain embodiments, n is as defined herein. In certain particularembodiments, n is between 1 and 10, inclusive. In a particularembodiment, n is about 5.

In a particular embodiments, Polymer (4) is of Formula B4: m=3, n isabout 5, each X is —NH—, and R₃ and R₄ are both

In a particular embodiments, Polymer (4) is of Formula C4: m=5, n isabout 5, each X is —NH—, and R₃ and R₄ are both

In a particular embodiments, Polymer (4) is of Formula D4: m=7, n isabout 5, each X is —NH—, and R₃ and R₄ are both

In certain embodiments, Polymer (4) is selected from B4, C4, and D4.

Polymer Properties

Exemplary polymers may be described in terms of properties including,weight average molecular weight (M_(w)), number average molecular weight(M_(n)), average hydrodynamic diameter (D_(H)), and polydispersity (Ð).

In certain embodiments, the M_(n) is determined with viscometry via the(Mark-Houwink equation), colligative methods (such as vapor pressureosmometry), end-group determination, or proton NMR. In certainembodiments, the M_(w) is determined with gel permeation chromatography,static light scattering, small angle neutron scattering, X-rayscattering, and sedimentation velocity.

In some embodiments, M_(w) is about 1000 to about 5000 Da, e.g., asdetermined by gel permeation chromatography. In some embodiments, M_(W)is about 1000 to about 3000 Da. In some embodiments, M_(W) is about 1000to about 2000 Da. In some embodiments, M_(W) is about 2000 to about 3000Da. See, e.g., FIG. 9.

In some embodiments, M_(w) of the polymer is between about 1 kDa andabout 100 kDa, e.g., between about 1 kDa and about 50 kDa, about 15 kDaand about 85 kDa, about 20 kDa and about 60 kDa, or about 30 kDa andabout 50 kDa. In one embodiment, the average molecular weight of thepolymer is between about 20 kDa and about 60 kDa. In one embodiment, theaverage molecular weight of the polymer is between about 30 kDa andabout 50 kDa.

In some embodiments, the polydispersity (Ð) of the polymer is between 1and 3, inclusive. In some embodiments, the polydispersity (Ð) of thepolymer is between 2 and 3, inclusive. In some embodiments, the averagepolydispersity of the polymer is less than bout 2.6 (e.g., less thanabout 2.5, about 2.4, about 2.3, about 2.2, about 2.1, or less). See,e.g., FIG. 9.

Compounds

Also provided are polylactone derived diacrylate compounds that can beused to make PBAEs of the present disclosure. In one aspect, providedherein is a compound of Formula (VI):

wherein:

R is optionally substituted, cyclic or acyclic aliphatic; optionallysubstituted, cyclic or acyclic heteroaliphatic; or a combinationthereof;

each R′ independently is optionally substituted, cyclic or acyclicaliphatic; optionally substituted, cyclic or acyclic heteroaliphatic; ora combination thereof;

each R_(a) independently is hydrogen or optionally substitutedaliphatic;

X′ is O or NR_(y), wherein R_(y) is hydrogen or optionally substitutedaliphatic; and

m is an integer between 1 and 100, inclusive.

In certain embodiments, R is a substituted aliphatic. In certainembodiments, R is unsubstituted aliphatic. In certain embodiments, R issubstituted heteroaliphatic. In certain embodiments, R is unsubstitutedheteroaliphatic.

In certain embodiments, R′ is substituted aliphatic. In certainembodiments, R′ is unsubstituted aliphatic. In certain embodiments, R′is substituted heteroaliphatic. In certain embodiments, R′ isunsubstituted heteroaliphatic.

In certain embodiments, R′ is of the formula:

wherein R₅ is selected from the group consisting of alkyl, heteroalkyl,alkenyl, alkynyl, haloalkyl, alkoxy, thioalkoxy, amino, alkylamino,dialkylamino, trialkylamino, acylamino, cyano, hydroxy, halo, mercapto,nitro, carboxyaldehyde, carboxy, alkoxycarbonyl, and carboxamide,wherein said alkyl, heteroalkyl, alkenyl, and alkynyl are optionallysubstituted, cyclic or acyclic, and branched or linear. In certainembodiments, R₅ is optionally substituted C₂-C₂₀ alkyl or optionallysubstituted C₂-C₂₀ heteroalkyl. In certain embodiments, r is 1-19.

In certain embodiments, X′ is O. In certain embodiments, X′ is NR_(y).In certain embodiments, X′ is NH.

In certain embodiments, m is selected from the group of integersconsisting of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, and 30. In certainembodiments, m is an integer between 31 and 40, inclusive. In certainembodiments, m is an integer between 41 and 50, inclusive. In certainembodiments, m is an integer between 51 and 60, inclusive. In certainembodiments, m is an integer between 61 and 70, inclusive. In certainembodiments, m is an integer between 71 and 80, inclusive. In certainembodiments, m is an integer between 81 and 90, inclusive. In certainembodiments, m is an integer between 91 and 100, inclusive. In certainembodiments, m is 3, 5 or 7.

In certain embodiments, the compound is of the formula:

In certain embodiments, the compound is of the formula:

In certain embodiments, the compound is of the formula:

Methods of Preparing Polymers and Compounds

The present disclosure provides methods of preparing PBAE polymers ofFormula (II), Formula (III), Formula (IV), and Formula (V), andcompounds of Formula (VI).

In one aspect, provided herein is a method of preparing a polymer ofFormula (II) as described herein:

or a pharmaceutically acceptable salt thereof, comprising reacting apolymer of Formula (IV) as described herein:

or a pharmaceutically acceptable salt thereof, with one or morenucleophiles selected from R₃XH and R₄XH, to obtain the polymer ofFormula (II).

In another aspect, provided herein is a method of preparing a polymer ofFormula (III) as described herein:

or a pharmaceutically acceptable salt thereof, comprising reacting apolymer of Formula (V) as described herein:

or a pharmaceutically acceptable salt thereof, with one or morenucleophiles selected from R₃XH and R₄XH; to obtain the polymer ofFormula (III).

In another aspect, provided herein is a method of preparing a polymer ofFormula (IV) as described herein:

or a pharmaceutically acceptable salt thereof, comprising reacting acompound of Formula (VI) as described herein:

with one or more amines of the formula R₁NH₂, to obtain the polymer ofFormula (IV).

In yet another aspect, provided herein is a method of preparing apolymer of Formula (V) as described herein:

or a pharmaceutically acceptable salt thereof, comprising reacting acompound of Formula (VI) as described herein:

with one or more diamines of the formula

to obtain the polymer of Formula (IV).

In another aspect, provided herein is a method of preparing a compoundof Formula (VI) as described herein:

comprising:

reacting a compound of Formula (VIa):

with a lactone to obtain a compound of Formula (VIb):

and

acylating the compound of Formula (VIb) to obtain the compound ofFormula (VI).

Any lactone may be used in the methods provided above. In certainembodiments, the lactone is of the formula:

wherein R′ is defined herein.

In certain embodiments, the lactone is selected from the groupconsisting of:

In certain embodiments, step of reacting comprises reacting a compoundof Formula (VIa) with a lactone in the presence of a catalyst to obtaina compound of Formula (VIb). In certain embodiments, the catalyst is aLewis acid. In certain embodiments, the Lewis acid is tin(II)2-ethylhexanoate.

In certain embodiments, step of acylating comprises acylating thecompound of Formula (VIb) with an electrophile of the formula:

wherein LG is a leaving group, to obtain the compound of Formula (VI).

In certain embodiments, the acylating reagent is an acryloyl halide. Incertain embodiments, the acylating agent is acryloyl chloride.

In certain embodiments, step of acylating further comprises acylatingthe compound of Formula (VIb) in the presence of a base. In certainembodiments, the base is a trialkylamine (e.g., trimethylamine,diisopropylethylamine, and the like).

The reactions described herein, which constitute the methods ofpreparing the compounds and polymers of the present disclosure, mayfurther include the use of one or more solvents. Common solvents usefulin the methods described herein include, but are not limited to,acetone, acetonitrile, benzene, benzonitrile, 1-butanol, 2-butanone,butyl acetate, tert-butyl methyl ether, carbon disulfide carbontetrachloride, chlorobenzene, 1-chlorobutane, chloroform, cyclohexane,cyclopentane, 1,2-dichlorobenzene, 1,2-dichloroethane, dichloromethane(DCM), N,N-dimethylacetamide N,N-dimethylformamide (DMF),1,3-dimethyl-3,4,5,6-tetrahydro-2-pyrimidinone (DMPU), 1,4-dioxane,1,3-dioxane, diethylether, 2-ethoxyethyl ether, ethyl acetate, ethylalcohol, ethylene glycol, dimethyl ether, heptane, n-hexane, hexanes,hexamethylphosphoramide (HMPA), 2-methoxyethanol, 2-methoxyethylacetate, methyl alcohol, 2-methylbutane, 4-methyl-2-pentanone,2-methyl-1-propanol, 2-methyl-2-propanol, 1-methyl-2-pyrrolidinone,dimethylsulfoxide (DMSO), nitromethane, 1-octanol, pentane, 3-pentanone,1-propanol, 2-propanol, pyridine, tetrachloroethylene, tetrahyrdofuran(THF), 2-methyltetrahydrofuran, toluene, trichlorobenzene,1,1,2-trichlorotrifluoroethane, 2,2,4-trimethylpentane, trimethylamine,triethylamine, N,N-diisopropylethylamine, diisopropylamine, water,o-xylene, and p-xylene. In certain embodiments, each reactionindependently is comprised of 1 to 10 solvents, inclusive. In certainembodiments, the reaction is comprised of one solvent. In certainembodiments, at least one solvent is dichloromethane (DCM). In certainembodiments, at least one solvent is dimethylsulfoxide (DMSO).

The reactions described herein, which constitute the methods ofpreparing the compounds and polymers of the present disclosure, areperformed at temperatures ranging between −10° C. and 150° C. In certainembodiments, the reaction is performed at temperatures below or at 50°C. In certain embodiments, the reaction is performed at temperaturesabove 50° C. In certain embodiments, the reaction is performed atapproximately 0° C. In certain embodiments, the reaction is performed atroom temperature. In certain embodiments, the reaction is performed atapproximately 60° C. In certain embodiments, the reaction is performedat approximately 90° C. In certain embodiments, the reaction isperformed at approximately 130° C.

The reactions described herein, which constitute the methods ofpreparing the compounds and polymers of the present disclosure, areperformed for about 1 hour to about 120 hours. In certain embodiments,the reaction is performed for about 3 hours to about 72 hours. Incertain embodiments, the reaction is performed for about 3 hours. Incertain embodiments, the reaction is performed for about 24 hours. Incertain embodiments, the reaction is performed for about 48 hours. Incertain embodiments, the reaction is performed for about 72 hours.

In certain embodiments, the preparation of a compound of Formula (VI):

comprises reacting a compound of Formula (VIa):

a lactone, a Lewis acid, and an acylating agent. Specific reactionconditions can be determined by those of ordinary skill in the art usingno more than routine experimentation.

In certain embodiments, the compound of Formula (VIa):

hydroxyethyl acrylate (HEA).

The lactone is used in an amount of about 1 to 15 equivalents of acompound of Formula (VIa). In certain embodiments, the lactone is usedin an amount of about 1 to 10 equivalents of a compound of Formula(VIa). In certain embodiments, the lactone is used in an amount of about1 to 7 equivalents of a compound of Formula (VIa). In certainembodiments, the lactone is used in an amount of about 1 to 5equivalents of a compound of Formula (VIa). In certain embodiments, thelactone is used in an amount of about 1 to 3 equivalents of a compoundof Formula (VIa). In certain embodiments, the lactone is used in anamount of about 7 equivalents of a compound of Formula (VIa). In certainembodiments, the lactone is used in an amount of about 5 equivalents ofa compound of Formula (VIa). In certain embodiments, the lactone is usedin an amount of about 3 equivalents of a compound of Formula (VIa).

The compound of Formula (VIa) is used in an amount of about 1 to 500equivalents of the Lewis acid. In certain embodiments, the compound ofFormula (VIa) is used in an amount of about 1 to 200 equivalents of theLewis acid. In certain embodiments, the compound of Formula (VIa) isused in an amount of about 200 equivalents of the Lewis acid.

In certain embodiments, the preparation of a polymer of Formula (IV)comprises reacting a compound of Formula (VI), or a pharmaceuticallyacceptable salt thereof, with one or more amines of the formula: R₁NH₂.In certain embodiments, the preparation of a polymer of Formula (V)comprises reacting a compound of Formula (VI), or a pharmaceuticallyacceptable salt thereof, with one or more diamines of the formula:

In certain embodiments, the preparation of a polymer of Formula (II), ora pharmaceutically acceptable salt thereof, comprises the reaction of apolymer of Formula (IV), or a pharmaceutically acceptable salt thereof,with one or more amines selected from R₃NH₂ and R₄NH₂. In certainembodiments, the preparation of a polymer of Formula (III), or apharmaceutically acceptable salt thereof, comprises the reaction of apolymer of Formula (V), or a pharmaceutically acceptable salt thereof,with one or more amines selected from R₃NH₂ and R₄NH₂, as describedherein.

In certain embodiments, the amine or diamine is selected from the groupconsisting of dodecyl amine, 3-amino-1,2-propanediol, 5-amino-pentanol,N,N-dimethylethylenediamine, 2-morpholinoethylamine, and2,2′-((oxybis(ethane-2,1-diyl))bis(oxy))diethanamine.

Compositions and Kits

The present disclosure provides compositions (e.g., pharmaceuticalcompositions) comprising a polymer described herein, and an excipient(e.g., pharmaceutically acceptable excipient). In certain embodiments,the composition is a pharmaceutical composition. In certain embodiments,the composition is a cosmetic composition. In certain embodiments, thecomposition is a nutraceutical composition. In certain embodiments, thecomposition is a composition with a non-medical application. In certainembodiments, the excipient is a pharmaceutically acceptable excipient.

Compositions described herein can be prepared by any method known in theart. In general, such preparatory methods include bringing the polymerdescribed herein into association with one or more excipients, and mayinclude one or more agents and then, if necessary and/or desirable,shaping, and/or packaging the product into a desired single- ormulti-dose unit. In certain embodiments, the agent and the polymer ofthe composition are not covalently attached.

In certain embodiments, the composition is in the form of a particle. Incertain embodiments, the particle is a nanoparticle or a microparticle.In certain embodiments, the particle is a micelle, liposome, orlipoplex. In certain embodiments, the particle encapsulates an agent, asdescribed herein. In certain embodiments, the particle facilitatesdelivery of the agent to a cell. See, e.g., FIGS. 5 and 10. In certainembodiments, the particle facilitates delivery of the agent to asubject, e.g., a human.

Nanoparticles and nanoparticle formulations are described herein. Incertain embodiments, the nanoparticle comprises one or more additionallipids. In certain particular embodiments, the nanoparticle comprises aPEG-lipid. As described herein, PCL-based PBAE nanoparticles can beprepared using a pre-mixing protocol or a direct-mixing protocol. See,e.g., FIGS. 10 and 11.

Compositions can be prepared, packaged, and/or sold in bulk, as a singleunit dose, and/or as a plurality of single unit doses. A “unit dose” isa discrete amount of the composition comprising a predetermined amountof the agent. The amount of the agent is generally equal to the dosageof the agent which would be administered to a subject and/or aconvenient fraction of such a dosage, such as one-half or one-third ofsuch a dosage.

Relative amounts of the polymer, excipient, agent, and/or any additionalingredients in a composition described herein will vary, depending uponthe identity, size, and/or condition of the subject treated and furtherdepending upon the route by which the composition is to be administered.The composition may comprise between 0.1% and 100% (w/w) agent.

Excipients and accessory ingredients used in the manufacture of providedcompositions include inert diluents, dispersing and/or granulatingagents, surface active agents and/or emulsifiers, disintegrating agents,binding agents, preservatives, buffering agents, lubricating agents,and/or oils. Excipients and accessory ingredients, such as cocoa butter,PEGylated lipids, phospholipids, suppository waxes, coloring agents,coating agents, sweetening, flavoring, and perfuming agents, may also bepresent in the composition.

Exemplary diluents include calcium carbonate, sodium carbonate, calciumphosphate, dicalcium phosphate, calcium sulfate, calcium hydrogenphosphate, sodium phosphate lactose, sucrose, cellulose,microcrystalline cellulose, kaolin, mannitol, sorbitol, inositol, sodiumchloride, dry starch, cornstarch, powdered sugar, and mixtures thereof.

Exemplary granulating and/or dispersing agents include potato starch,corn starch, tapioca starch, sodium starch glycolate, clays, alginicacid, guar gum, citrus pulp, agar, bentonite, cellulose, and woodproducts, natural sponge, cation-exchange resins, calcium carbonate,silicates, sodium carbonate, cross-linked poly(vinyl-pyrrolidone)(crospovidone), sodium carboxymethyl starch (sodium starch glycolate),carboxymethyl cellulose, cross-linked sodium carboxymethyl cellulose(croscarmellose), methylcellulose, pregelatinized starch (starch 1500),microcrystalline starch, water insoluble starch, calcium carboxymethylcellulose, magnesium aluminum silicate (Veegum), sodium lauryl sulfate,quaternary ammonium compounds, and mixtures thereof.

Exemplary surface active agents and/or emulsifiers include naturalemulsifiers (e.g., acacia, agar, alginic acid, sodium alginate,tragacanth, chondrux, xanthan, pectin, gelatin, egg yolk, casein, woolfat, cholesterol, wax, and lecithin), colloidal clays (e.g., bentonite(aluminum silicate) and Veegum (magnesium aluminum silicate)), longchain amino acid derivatives, high molecular weight alcohols (e.g.,stearyl alcohol, cetyl alcohol, oleyl alcohol, triacetin monostearate,ethylene glycol distearate, glyceryl monostearate, and propylene glycolmonostearate, polyvinyl alcohol), carbomers (e.g., carboxypolymethylene, polyacrylic acid, acrylic acid polymer, and carboxyvinylpolymer), carrageenan, cellulosic derivatives (e.g.,carboxymethylcellulose sodium, powdered cellulose, hydroxymethylcellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose,methylcellulose), sorbitan fatty acid esters (e.g., polyoxyethylenesorbitan monolaurate (Tween® 20), polyoxyethylene sorbitan monostearate(Tween® 60), polyoxyethylene sorbitan monooleate (Tween® 80), sorbitanmonopalmitate (Span® 40), sorbitan monostearate (Span® 60), sorbitantristearate (Span® 65), glyceryl monooleate, sorbitan monooleate (Span®80), polyoxyethylene esters (e.g., polyoxyethylene monostearate (Myrj®45), polyoxyethylene hydrogenated castor oil, polyethoxylated castoroil, polyoxymethylene stearate, and Solutol®), sucrose fatty acidesters, polyethylene glycol fatty acid esters (e.g., Cremophor®),polyoxyethylene ethers, (e.g., polyoxyethylene lauryl ether (Brij® 30)),poly(vinyl-pyrrolidone), diethylene glycol monolaurate, triethanolamineoleate, sodium oleate, potassium oleate, ethyl oleate, oleic acid, ethyllaurate, sodium lauryl sulfate, Pluronic® F-68, poloxamer P-188,cetrimonium bromide, cetylpyridinium chloride, benzalkonium chloride,docusate sodium, and/or mixtures thereof.

Exemplary binding agents include starch (e.g., cornstarch and starchpaste), gelatin, sugars (e.g., sucrose, glucose, dextrose, dextrin,molasses, lactose, lactitol, mannitol, etc.), natural and synthetic gums(e.g., acacia, sodium alginate, extract of Irish moss, panwar gum,ghatti gum, mucilage of isapol husks, carboxymethylcellulose,methylcellulose, ethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, hydroxypropyl methylcellulose, microcrystalline cellulose,cellulose acetate, poly(vinyl-pyrrolidone), magnesium aluminum silicate(Veegum®), and larch arabogalactan), alginates, polyethylene oxide,polyethylene glycol, inorganic calcium salts, silicic acid,polymethacrylates, waxes, water, alcohol, and/or mixtures thereof.

Exemplary preservatives include antioxidants, chelating agents,antimicrobial preservatives, antifungal preservatives, antiprotozoanpreservatives, alcohol preservatives, acidic preservatives, and otherpreservatives. In certain embodiments, the preservative is anantioxidant. In other embodiments, the preservative is a chelatingagent.

Exemplary antioxidants include alpha tocopherol, ascorbic acid, acorbylpalmitate, butylated hydroxyanisole, butylated hydroxytoluene,monothioglycerol, potassium metabisulfite, propionic acid, propylgallate, sodium ascorbate, sodium bisulfite, sodium metabisulfite, andsodium sulfite.

Exemplary chelating agents include ethylenediaminetetraacetic acid(EDTA) and salts and hydrates thereof (e.g., sodium edetate, disodiumedetate, trisodium edetate, calcium disodium edetate, dipotassiumedetate, and the like), citric acid and salts and hydrates thereof(e.g., citric acid monohydrate), fumaric acid and salts and hydratesthereof, malic acid and salts and hydrates thereof, phosphoric acid andsalts and hydrates thereof, and tartaric acid and salts and hydratesthereof. Exemplary antimicrobial preservatives include benzalkoniumchloride, benzethonium chloride, benzyl alcohol, bronopol, cetrimide,cetylpyridinium chloride, chlorhexidine, chlorobutanol, chlorocresol,chloroxylenol, cresol, ethyl alcohol, glycerin, hexetidine, imidurea,phenol, phenoxyethanol, phenylethyl alcohol, phenylmercuric nitrate,propylene glycol, and thimerosal.

Exemplary antifungal preservatives include butyl paraben, methylparaben, ethyl paraben, propyl paraben, benzoic acid, hydroxybenzoicacid, potassium benzoate, potassium sorbate, sodium benzoate, sodiumpropionate, and sorbic acid.

Exemplary alcohol preservatives include ethanol, polyethylene glycol,phenol, phenolic compounds, bisphenol, chlorobutanol, hydroxybenzoate,and phenylethyl alcohol.

Exemplary acidic preservatives include vitamin A, vitamin C, vitamin E,beta-carotene, citric acid, acetic acid, dehydroacetic acid, ascorbicacid, sorbic acid, and phytic acid.

Other preservatives include tocopherol, tocopherol acetate, deteroximemesylate, cetrimide, butylated hydroxyanisol (BHA), butylatedhydroxytoluened (BHT), ethylenediamine, sodium lauryl sulfate (SLS),sodium lauryl ether sulfate (SLES), sodium bisulfite, sodiummetabisulfite, potassium sulfite, potassium metabisulfite, Glydant®Plus, Phenonip®, methylparaben, Germall® 115, Germaben® II, Neolone®,Kathon®, and Euxyl®.

Exemplary buffering agents include citrate buffer solutions, acetatebuffer solutions, phosphate buffer solutions, ammonium chloride, calciumcarbonate, calcium chloride, calcium citrate, calcium glubionate,calcium gluceptate, calcium gluconate, D-gluconic acid, calciumglycerophosphate, calcium lactate, propanoic acid, calcium levulinate,pentanoic acid, dibasic calcium phosphate, phosphoric acid, tribasiccalcium phosphate, calcium hydroxide phosphate, potassium acetate,potassium chloride, potassium gluconate, potassium mixtures, dibasicpotassium phosphate, monobasic potassium phosphate, potassium phosphatemixtures, sodium acetate, sodium bicarbonate, sodium chloride, sodiumcitrate, sodium lactate, dibasic sodium phosphate, monobasic sodiumphosphate, sodium phosphate mixtures, tromethamine, magnesium hydroxide,aluminum hydroxide, alginic acid, pyrogen-free water, isotonic saline,Ringer's solution, ethyl alcohol, and mixtures thereof.

Exemplary lubricating agents include magnesium stearate, calciumstearate, stearic acid, silica, talc, malt, glyceryl behanate,hydrogenated vegetable oils, polyethylene glycol, sodium benzoate,sodium acetate, sodium chloride, leucine, magnesium lauryl sulfate,sodium lauryl sulfate, and mixtures thereof.

Exemplary natural oils include almond, apricot kernel, avocado, babassu,bergamot, black current seed, borage, cade, camomile, canola, caraway,carnauba, castor, cinnamon, cocoa butter, coconut, cod liver, coffee,corn, cotton seed, emu, Eucalyptus, evening primrose, fish, flaxseed,geraniol, gourd, grape seed, hazel nut, hyssop, isopropyl myristate,jojoba, kukui nut, lavandin, lavender, lemon, Litsea cubeba, macademianut, mallow, mango seed, meadowfoam seed, mink, nutmeg, olive, orange,orange roughy, palm, palm kernel, peach kernel, peanut, poppy seed,pumpkin seed, rapeseed, rice bran, rosemary, safflower, sandalwood,sasquana, savoury, sea buckthorn, sesame, shea butter, silicone,soybean, sunflower, tea tree, thistle, tsubaki, vetiver, walnut, andwheat germ oils. Exemplary synthetic oils include butyl stearate,caprylic triglyceride, capric triglyceride, cyclomethicone, diethylsebacate, dimethicone 360, isopropyl myristate, mineral oil,octyldodecanol, oleyl alcohol, silicone oil, and mixtures thereof.

In certain embodiments, the compositions, further comprise an agent, andare useful for delivering said agent (e.g., to a subject or cell). Incertain embodiments, the compositions are pharmaceutical compositionswhich are useful for treating a disease in a subject in need thereof. Incertain embodiments, the pharmaceutical compositions are useful forpreventing a disease in a subject. In certain embodiments, thepharmaceutical compositions are useful for diagnosing a disease in asubject.

A composition as described herein may further comprise, or can beadministered in combination with, one or more additional agents. Incertain embodiments, the agent is a small organic molecule, inorganicmolecule, nucleic acid, protein, peptide, or polynucleotide. In certainembodiments, the agent is a pharmaceutical agent (e.g., therapeuticallyand/or prophylactically active agent). Pharmaceutical agents includetherapeutically active agents. Pharmaceutical agents also includeprophylactically active agents. Pharmaceutical agents include smallorganic molecules such as drug compounds (e.g., compounds approved forhuman or veterinary use by the U.S. Food and Drug Administration asprovided in the Code of Federal Regulations (CFR)), peptides, proteins,carbohydrates, monosaccharides, oligosaccharides, polysaccharides,nucleoproteins, mucoproteins, lipoproteins, synthetic polypeptides orproteins, small molecules linked to proteins, glycoproteins, steroids,nucleic acids, DNAs, RNAs, nucleotides, nucleosides, oligonucleotides,antisense oligonucleotides, polynucleotides, lipids, hormones, vitamins,vaccines, immunological agents, and cells or other biological materials.

In certain embodiments, the agent is a polynucleotide. In certainembodiments, the polynucleotide is DNA. In certain embodiments, thepolynucleotide is RNA. In certain embodiments, the polynucleotidecarries out RNA interference. The RNA is selected from the groupconsisting of double-stranded RNA (dsRNA), small interfering RNA(siRNA), short hairpin (shRNA), microRNA (miRNA), messenger RNA (mRNA),antisense RNA, transfer RNA (tRNA), small nuclear RNA (snRNA), andribosomal RNA (rRNA). In certain embodiments, the RNA is dsRNA. Incertain embodiments, the RNA is siRNA. In certain embodiments, the RNAis shRNA. In certain embodiments, the RNA is miRNA. In certainembodiments, the RNA is mRNA. In certain embodiments, the RNA isantisense RNA.

In certain embodiments, the agent described herein is provided in aneffective amount in the composition. In certain embodiments, theeffective amount is a therapeutically effective amount. In certainembodiments, the effective amount is a prophylactically effectiveamount. In certain embodiments, the effective amount is an amounteffective for treating a proliferative disease in a subject in needthereof. In certain embodiments, the effective amount is an amounteffective for preventing a proliferative disease in a subject in needthereof. In certain embodiments, the effective amount is an amounteffective for treating a hematological disease in a subject in needthereof. In certain embodiments, the effective amount is an amounteffective for preventing a hematological disease in a subject in needthereof. In certain embodiments, the effective amount is an amounteffective for treating a neurological disease in a subject in needthereof. In certain embodiments, the effective amount is an amounteffective for preventing a neurological disease in a subject in needthereof. In certain embodiments, the effective amount is an amounteffective for treating a in a painful condition subject in need thereof.In certain embodiments, the effective amount is an amount effective forpreventing a painful condition in a subject in need thereof. In certainembodiments, the effective amount is an amount effective for treating apsychiatric disorder in a subject in need thereof. In certainembodiments, the effective amount is an amount effective for preventinga psychiatric disorder in a subject in need thereof. In certainembodiments, the effective amount is an amount effective for treating ametabolic disorder in a subject in need thereof. In certain embodiments,the effective amount is an amount effective for preventing a metabolicdisorder in a subject in need thereof. In certain embodiments, theeffective amount is an amount effective for reducing the risk ofdeveloping a disease (e.g., proliferative disease, hematologicaldisease, neurological disease, infectious disease, inflammatory disease,autoimmune disease, gastrointestinal disease, liver disease, lungdisease, kidney disease, spleen disease, familial amyloid neuropathies,painful condition, psychiatric disorder, or metabolic disorder) in asubject in need thereof.

In certain embodiments, the cell is in vitro. In certain embodiments,the cell is in vivo.

Compositions may be formulated into liquid dosage forms for oral andparenteral administration include pharmaceutically acceptable emulsions,microemulsions, solutions, suspensions, syrups and elixirs. In additionto the agents, the liquid dosage forms may comprise inert diluentscommonly used in the art such as, for example, water or other solvents,solubilizing agents and emulsifiers such as ethyl alcohol, isopropylalcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzylbenzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils(e.g., cottonseed, groundnut, corn, germ, olive, castor, and sesameoils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols andfatty acid esters of sorbitan, and mixtures thereof. Besides inertdiluents, the oral compositions can include adjuvants such as wettingagents, emulsifying and suspending agents, sweetening, flavoring, andperfuming agents. In certain embodiments for parenteral administration,the particles described herein are mixed with solubilizing agents, suchas Cremophor®, alcohols, oils, modified oils, glycols, polysorbates,cyclodextrins, polymers, and mixtures thereof.

Injectable preparations, for example, sterile injectable aqueous oroleaginous suspensions can be formulated according to the known artusing suitable dispersing or wetting agents and suspending agents. Thesterile injectable preparation can be a sterile injectable solution,suspension, or emulsion in a nontoxic parenterally acceptable diluent orsolvent, for example, as a solution in 1,3-butanediol. Among theacceptable vehicles and solvents that can be employed are water,Ringer's solution, U.S.P., and isotonic sodium chloride solution. Inaddition, sterile, fixed oils are conventionally employed as a solventor suspending medium. For this purpose any bland fixed oil can beemployed including synthetic mono- or di-glycerides. In addition, fattyacids, such as oleic acid, are used in the preparation of injectables.

The injectable formulations can be sterilized, for example, byfiltration through a bacterial-retaining filter, or by incorporatingsterilizing agents in the form of sterile solid compositions which canbe dissolved or dispersed in sterile water or other sterile injectablemedium prior to use.

In order to prolong the effect of an agent, it is often desirable toslow the absorption of the drug from subcutaneous or intramuscularinjection. This can be accomplished by the use of a liquid suspension ofcrystalline or amorphous material with poor water solubility. The rateof absorption of the agent then depends upon its rate of dissolution,which, in turn, may depend upon crystal size and crystalline form.Alternatively, delayed absorption of a parenterally administered drugform may be accomplished by dissolving or suspending the agent in an oilvehicle.

Compositions for rectal or vaginal administration are typicallysuppositories which can be prepared by mixing the particles describedherein with suitable non-irritating excipients or carriers such as cocoabutter, polyethylene glycol, or a suppository wax which are solid atambient temperature but liquid at body temperature and therefore melt inthe rectum or vaginal cavity and release the agent.

Compositions may be formulated into solid dosage forms for oraladministration include capsules, tablets, pills, powders, and granules.In such solid dosage forms, the agent is mixed with at least one inert,pharmaceutically acceptable excipient or carrier such as sodium citrateor dicalcium phosphate and/or (a) fillers or extenders such as starches,lactose, sucrose, glucose, mannitol, and silicic acid, (b) binders suchas, for example, carboxymethylcellulose, alginates, gelatin,polyvinylpyrrolidinone, sucrose, and acacia, (c) humectants such asglycerol, (d) disintegrating agents such as agar, calcium carbonate,potato or tapioca starch, alginic acid, certain silicates, and sodiumcarbonate, (e) solution retarding agents such as paraffin, (f)absorption accelerators such as quaternary ammonium compounds, (g)wetting agents such as, for example, cetyl alcohol and glycerolmonostearate, (h) absorbents such as kaolin and bentonite clay, and (i)lubricants such as talc, calcium stearate, magnesium stearate, solidpolyethylene glycols, sodium lauryl sulfate, and mixtures thereof. Inthe case of capsules, tablets, and pills, the dosage form may include abuffering agent.

Solid compositions of a similar type can be employed as fillers in softand hard-filled gelatin capsules using such excipients as lactose ormilk sugar as well as high molecular weight polyethylene glycols and thelike. The solid dosage forms of tablets, dragees, capsules, pills, andgranules can be prepared with coatings and shells such as entericcoatings and other coatings well known in the art of pharmacology. Theymay optionally comprise opacifying agents and can be of a compositionthat they release the agent(s) only, or preferentially, in a certainpart of the intestinal tract, optionally, in a delayed manner. Examplesof encapsulating compositions which can be used include polymericsubstances and waxes. Solid compositions of a similar type can beemployed as fillers in soft and hard-filled gelatin capsules using suchexcipients as lactose or milk sugar as well as high molecular weightpolyethylene glycols and the like.

The agent can be in a micro-encapsulated form with one or moreexcipients as noted above. The solid dosage forms of tablets, dragees,capsules, pills, and granules can be prepared with coatings and shellssuch as enteric coatings, release controlling coatings, and othercoatings well known in the pharmaceutical formulating art. In such soliddosage forms the agent can be admixed with at least one inert diluentsuch as sucrose, lactose, or starch. Such dosage forms may comprise, asis normal practice, additional substances other than inert diluents,e.g., tableting lubricants and other tableting aids such a magnesiumstearate and microcrystalline cellulose. In the case of capsules,tablets and pills, the dosage forms may comprise buffering agents. Theymay optionally comprise opacifying agents and can be of a compositionthat they release the agent(s) only, or preferentially, in a certainpart of the intestinal tract, optionally, in a delayed manner. Examplesof encapsulating agents which can be used include polymeric substancesand waxes.

Dosage forms for topical and/or transdermal administration of acomposition described herein may include ointments, pastes, creams,lotions, gels, powders, solutions, sprays, inhalants, and/or patches.Generally, the agent is admixed under sterile conditions with apharmaceutically acceptable carrier or excipient and/or any neededpreservatives and/or buffers as can be required. Additionally, thepresent disclosure contemplates the use of transdermal patches, whichoften have the added advantage of providing controlled delivery of anagent to the body. Such dosage forms can be prepared, for example, bydissolving and/or dispensing the agent in the proper medium.Alternatively or additionally, the rate can be controlled by eitherproviding a rate controlling membrane and/or by dispersing the agent ina polymer matrix and/or gel.

Suitable devices for use in delivering intradermal compositionsdescribed herein include short needle devices. Intradermal compositionscan be administered by devices which limit the effective penetrationlength of a needle into the skin. Alternatively or additionally,conventional syringes can be used in the classical mantoux method ofintradermal administration. Jet injection devices which deliver liquidformulations to the dermis via a liquid jet injector and/or via a needlewhich pierces the stratum corneum and produces a jet which reaches thedermis are suitable. Ballistic powder/particle delivery devices whichuse compressed gas to accelerate the polymer in powder form through theouter layers of the skin to the dermis are suitable.

Formulations suitable for topical administration include liquid and/orsemi-liquid preparations such as liniments, lotions, oil-in-water and/orwater-in-oil emulsions such as creams, ointments, and/or pastes, and/orsolutions and/or suspensions. Topically administrable formulations may,for example, comprise from about 1% to about 10% (w/w) agent, althoughthe concentration of the agent can be as high as the solubility limit ofthe agent in the solvent. Formulations for topical administration mayfurther comprise one or more of the additional ingredients describedherein.

A composition described herein can be prepared, packaged, and/or sold ina formulation suitable for pulmonary administration via the buccalcavity. Such a formulation may comprise dry particles which comprise theagent and which have a diameter in the range from about 0.5 to about 7nanometers, or from about 1 to about 6 nanometers. Such compositions areconveniently in the form of dry powders for administration using adevice comprising a dry powder reservoir to which a stream of propellantcan be directed to disperse the powder and/or using a self-propellingsolvent/powder dispensing container such as a device comprising theagent dissolved and/or suspended in a low-boiling propellant in a sealedcontainer. Such powders comprise particles wherein at least 98% of theparticles by weight have a diameter greater than 0.5 nanometers and atleast 95% of the particles by number have a diameter less than 7nanometers. Alternatively, at least 95% of the particles by weight havea diameter greater than 1 nanometer and at least 90% of the particles bynumber have a diameter less than 6 nanometers. Dry powder compositionsmay include a solid fine powder diluent such as sugar and areconveniently provided in a unit dose form.

Low boiling propellants generally include liquid propellants having aboiling point of below 65° F. at atmospheric pressure. Generally thepropellant may constitute 50 to 99.9% (w/w) of the composition, and theagent may constitute 0.1 to 20% (w/w) of the composition. The propellantmay further comprise additional ingredients such as a liquid non-ionicand/or solid anionic surfactant and/or a solid diluent (which may have aparticle size of the same order as particles comprising the agent).

Compositions described herein formulated for pulmonary delivery mayprovide the agent in the form of droplets of a solution and/orsuspension. Such formulations can be prepared, packaged, and/or sold asaqueous and/or dilute alcoholic solutions and/or suspensions, optionallysterile, comprising the agent, and may conveniently be administeredusing any nebulization and/or atomization device. Such formulations mayfurther comprise one or more additional ingredients including aflavoring agent such as saccharin sodium, a volatile oil, a bufferingagent, a surface active agent, and/or a preservative such asmethylhydroxybenzoate.

Formulations described herein as being useful for pulmonary delivery areuseful for intranasal delivery of a pharmaceutical composition describedherein. Another formulation suitable for intranasal administration is acoarse powder comprising the agent and having an average particle fromabout 0.2 to 500 micrometers. Such a formulation is administered byrapid inhalation through the nasal passage from a container of thepowder held close to the nares.

Formulations for nasal administration may, for example, comprise fromabout as little as 0.1% (w/w) to as much as 100% (w/w) of the agent, andmay comprise one or more of the additional ingredients described herein.A pharmaceutical composition described herein can be prepared, packaged,and/or sold in a formulation for buccal administration. Suchformulations may, for example, be in the form of tablets and/or lozengesmade using conventional methods, and may contain, for example, 0.1 to20% (w/w) agent, the balance comprising an orally dissolvable and/ordegradable composition and, optionally, one or more of the additionalingredients described herein. Alternately, formulations for buccaladministration may comprise a powder and/or an aerosolized and/oratomized solution and/or suspension comprising the agent. Such powdered,aerosolized, and/or aerosolized formulations, when dispersed, may havean average particle and/or droplet size in the range from about 0.1 toabout 200 nanometers, and may further comprise one or more of theadditional ingredients described herein.

A composition described herein can be prepared, packaged, and/or sold ina formulation for ophthalmic administration. Such formulations may, forexample, be in the form of eye drops including, for example, a 0.1-1.0%(w/w) solution and/or suspension of the agent in an aqueous or oilyliquid carrier or excipient. Such drops may further comprise bufferingagents, salts, and/or one or more other of the additional ingredientsdescribed herein. Other ophthalmically-administrable formulations whichare useful include those which comprise the agent in microcrystallineform and/or in a liposomal preparation. Ear drops and/or eye drops arealso contemplated as being within the scope of this disclosure.

Although the descriptions of compositions provided herein areprincipally directed to compositions which are suitable foradministration to humans, it will be understood by the skilled artisanthat such compositions are generally suitable for administration toanimals of all sorts. Modification of compositions suitable foradministration to humans in order to render the compositions suitablefor administration to various animals is well understood, and theordinarily skilled veterinary pharmacologist can design and/or performsuch modification with ordinary experimentation.

Compositions provided herein are typically formulated in dosage unitform for ease of administration and uniformity of dosage. It will beunderstood, however, that the total daily usage of the compositionsdescribed herein will be decided by a physician within the scope ofsound medical judgment. The specific therapeutically effective doselevel for any particular subject or organism will depend upon a varietyof factors including the disease being treated and the severity of thedisorder; the activity of the specific agent employed; the specificcomposition employed; the age, body weight, general health, sex, anddiet of the subject; the time of administration, route ofadministration, and rate of excretion of the specific agent employed;the duration of the treatment; drugs used in combination or coincidentalwith the specific agent employed; and like factors well known in themedical arts.

The compositions provided herein can be administered by any route,including enteral (e.g., oral), parenteral, intravenous, intramuscular,intra-arterial, intramedullary, intrathecal, subcutaneous,intraventricular, transdermal, interdermal, rectal, intravaginal,intraperitoneal, topical (as by powders, ointments, creams, and/ordrops), mucosal, nasal, bucal, sublingual; by intratrachealinstillation, bronchial instillation, and/or inhalation; and/or as anoral spray, nasal spray, and/or aerosol. Specifically contemplatedroutes are oral administration, intravenous administration (e.g.,systemic intravenous injection), regional administration via bloodand/or lymph supply, and/or direct administration to an affected site.In general, the most appropriate route of administration will dependupon a variety of factors including the nature of the agent (e.g., itsstability in the environment of the gastrointestinal tract), and/or thecondition of the subject (e.g., whether the subject is able to tolerateoral administration). In certain embodiments, the composition describedherein is suitable for topical administration to the eye of a subject.

The exact amount of an agent required to achieve an effective amountwill vary from subject to subject, depending, for example, on species,age, and general condition of a subject, severity of the side effects ordisorder, identity of the particular agent, mode of administration, andthe like. An effective amount may be included in a single dose (e.g.,single oral dose) or multiple doses (e.g., multiple oral doses). Incertain embodiments, when multiple doses are administered to a subjector applied to a tissue or cell, any two doses of the multiple dosesinclude different or substantially the same amounts of an agentdescribed herein. In certain embodiments, when multiple doses areadministered to a subject or applied to a tissue or cell, the frequencyof administering the multiple doses to the subject or applying themultiple doses to the tissue or cell is three doses a day, two doses aday, one dose a day, one dose every other day, one dose every third day,one dose every week, one dose every two weeks, one dose every threeweeks, or one dose every four weeks. In certain embodiments, thefrequency of administering the multiple doses to the subject or applyingthe multiple doses to the tissue or cell is one dose per day. In certainembodiments, the frequency of administering the multiple doses to thesubject or applying the multiple doses to the tissue or cell is twodoses per day. In certain embodiments, the frequency of administeringthe multiple doses to the subject or applying the multiple doses to thetissue or cell is three doses per day. In certain embodiments, whenmultiple doses are administered to a subject or applied to a tissue orcell, the duration between the first dose and last dose of the multipledoses is one day, two days, four days, one week, two weeks, three weeks,one month, two months, three months, four months, six months, ninemonths, one year, two years, three years, four years, five years, sevenyears, ten years, fifteen years, twenty years, or the lifetime of thesubject, tissue, or cell. In certain embodiments, the duration betweenthe first dose and last dose of the multiple doses is three months, sixmonths, or one year. In certain embodiments, the duration between thefirst dose and last dose of the multiple doses is the lifetime of thesubject, tissue, or cell. In certain embodiments, a dose (e.g., a singledose, or any dose of multiple doses) described herein includesindependently between 0.1 μg and 1 μg, between 0.001 mg and 0.01 mg,between 0.01 mg and 0.1 mg, between 0.1 mg and 1 mg, between 1 mg and 3mg, between 3 mg and 10 mg, between 10 mg and 30 mg, between 30 mg and100 mg, between 100 mg and 300 mg, between 300 mg and 1,000 mg, orbetween 1 g and 10 g, inclusive, of a polymer described herein. Incertain embodiments, a dose described herein includes independentlybetween 1 mg and 3 mg, inclusive, of a polymer described herein. Incertain embodiments, a dose described herein includes independentlybetween 3 mg and 10 mg, inclusive, of a polymer described herein. Incertain embodiments, a dose described herein includes independentlybetween 10 mg and 30 mg, inclusive, of a polymer described herein. Incertain embodiments, a dose described herein includes independentlybetween 30 mg and 100 mg, inclusive, of a polymer described herein.

Dose ranges as described herein provide guidance for the administrationof provided compositions to an adult. The amount to be administered to,for example, a child or an adolescent can be determined by a medicalpractitioner or person skilled in the art and can be lower or the sameas that administered to an adult. In certain embodiments, a dosedescribed herein is a dose to an adult human whose body weight is 70 kg.

The compositions can be administered in combination with additionalagents that improve their activity (e.g., activity (e.g., potency and/orefficacy) in treating a disease in a subject in need thereof, inpreventing a disease in a subject in need thereof, in reducing the riskto develop a disease in a subject in need thereof, and/or in inhibitingthe activity of a protein kinase in a subject or cell), improvebioavailability, improve safety, reduce drug resistance, reduce and/ormodify metabolism, inhibit excretion, and/or modify distribution in asubject or cell. It will also be appreciated that the therapy employedmay achieve a desired effect for the same disorder, and/or it mayachieve different effects. In certain embodiments, a compositiondescribed herein including a polymer described herein and an agent showsa synergistic effect that is absent in a composition including one ofthe polymer and an agent, but not both.

The composition can be administered concurrently with, prior to, orsubsequent to one or more additional agents, which are different fromthe composition and may be useful as, e.g., combination therapies.Pharmaceutical agents include therapeutically active agents.Pharmaceutical agents also include prophylactically active agents.Pharmaceutical agents include small organic molecules such as drugcompounds (e.g., compounds approved for human or veterinary use by theU.S. Food and Drug Administration as provided in the Code of FederalRegulations (CFR)), peptides, proteins, carbohydrates, monosaccharides,oligosaccharides, polysaccharides, nucleoproteins, mucoproteins,lipoproteins, synthetic polypeptides or proteins, small molecules linkedto proteins, glycoproteins, steroids, nucleic acids, DNAs, RNAs,nucleotides, nucleosides, oligonucleotides, antisense oligonucleotides,lipids, hormones, vitamins, and cells. In certain embodiments, theadditional pharmaceutical agent is a pharmaceutical agent useful fortreating and/or preventing a disease (e.g., proliferative disease,hematological disease, neurological disease, painful condition,psychiatric disorder, or metabolic disorder). Each additionalpharmaceutical agent may be administered at a dose and/or on a timeschedule determined for that pharmaceutical agent. The additionalpharmaceutical agents may also be administered together with each otherand/or with the polymer or composition described herein in a single doseor administered separately in different doses. The particularcombination to employ in a regimen will take into account compatibilityof the polymer described herein with the additional pharmaceuticalagent(s) and/or the desired therapeutic and/or prophylactic effect to beachieved. In general, it is expected that the additional pharmaceuticalagent(s) in combination be utilized at levels that do not exceed thelevels at which they are utilized individually. In some embodiments, thelevels utilized in combination will be lower than those utilizedindividually.

The additional pharmaceutical agents include anti-proliferative agents,anti-cancer agents, cytotoxic agents, anti-angiogenesis agents,anti-inflammatory agents, immunosuppressants, anti-bacterial agents,anti-viral agents, cardiovascular agents, cholesterol-lowering agents,antidiabetic agents, anti-allergic agents, contraceptive agents, andpain-relieving agents. In certain embodiments, the additionalpharmaceutical agent is an anti-proliferative agent. In certainembodiments, the additional pharmaceutical agent is an anti-canceragent. In certain embodiments, the additional pharmaceutical agent is ananti-viral agent. In certain embodiments, the additional pharmaceuticalagent is a binder or inhibitor of a protein kinase. In certainembodiments, the additional pharmaceutical agent is selected from thegroup consisting of epigenetic or transcriptional modulators (e.g., DNAmethyltransferase inhibitors, histone deacetylase inhibitors (HDACinhibitors), lysine methyltransferase inhibitors), antimitotic drugs(e.g., taxanes and vinca alkaloids), hormone receptor modulators (e.g.,estrogen receptor modulators and androgen receptor modulators), cellsignaling pathway inhibitors (e.g., tyrosine protein kinase inhibitors),modulators of protein stability (e.g., proteasome inhibitors), Hsp90inhibitors, glucocorticoids, all-trans retinoic acids, and other agentsthat promote differentiation. In certain embodiments, the polymersdescribed herein or pharmaceutical compositions can be administered incombination with an anti-cancer therapy including surgery, radiationtherapy, transplantation (e.g., stem cell transplantation, bone marrowtransplantation), immunotherapy, and chemotherapy.

In some embodiments, the composition is a particle (e.g., ananoparticle). In some embodiments, the particle is substantiallysoluble in water (e.g., hydrophilic). In some embodiments, the particleis substantially insoluble in water (e.g., hydrophobic). In someembodiments, the particle is substantially insoluble in water andgreater than about 10,000 parts water are required to dissolve 1 partpolymer. In one embodiment, the particle is amphiphilic. In oneembodiment, the particle comprises a segment that is hydrophobic and asegment that is hydrophilic.

In some embodiments, the percentage of the particles that comprise anagent is between about 1 and about 100% (e.g., about 1%, about 2%, about3%, about 4%, about 5%, about 10%, about 15%, about 20%, about 30%,about 40%, about 50%, about 60%, about 70%, about 80%, about 90%, orabout 100%). In some embodiments, the percentage of the particles thatcomprise an agent is less than about 50%, e.g., less than about 40%,less than about 35%, less than about 30%, less than about 25%, less thanabout 20%, less than about 15%, or less than about 10%. In someembodiments, the percentage of the particles that comprise an agent isbetween about 5% and about 50%, about 5% and about 40%, about 5% andabout 30%, about 5% and about 25%, or about 5% and about 20%. In someembodiments, the percentage of the particles that comprise an agent isbetween about 5% and 90%. In some embodiments, the percentage of theparticles that comprise an agent is between about 5% and about 75%. Inthe some embodiments, the percentage of particles that comprise an agentis between about 5% and about 50%. In the some embodiments, thepercentage of the particles that comprise an agent is between about 10%and about 25%.

In some embodiments, the total amount of the agent present in theparticle is greater than about 5% (e.g., about 6%, about 7%, about 8%,about 9%, about 10%, about 12%, about 15%, about 20%, about 25%, about30%, or more) of the total size or weight of the conjugate or particle.In some embodiments, the total amount of the agent present in theconjugate or particle is greater than about 10% (e.g., about 12%, about15%, about 20%, about 25%, about 30%, or more) of the total size orweight of the conjugate or particle.

Without being bound by theory, the polymers or particles disclosedherein may improve the efficiency of an agent by one or more ofincreasing the localization and/or release (e.g., preferential release)of the agent to a target cell (e.g., a cancer or a fibrotic cell; a cellassociated with a hypoxic environment), or increasing the half life ofthe agent, thus resulting in a significantly higher amount of a releasedagent at a target site (e.g., a tumor or liver (e.g., cirrhotic cell).According, the conjugates and particles disclosed herein can be moreeffective therapeutically than the free agent (e.g., due to enhanceddrug uptake in the target tissue) and/or allow for a lower therapeuticdose of the agent, e.g., without substantially compromising theresulting drug concentration at a target tissue. In some embodiments,the conjugates and particles disclosed herein can reduce the adverseeffect associated with systemic administration of an agent in free form(e.g., not coupled to a polymer, conjugate or particle describedherein).

Without being bound by theory, due to the localized delivery of thecompositions described herein (e.g., the agent-containing particles), alower dose or amount of the agent in the particles can be administered(e.g., through local sustained delivery) compared to the agent in freeform. In other embodiments, the agent-containing particles areadministered at a dose or amount of the agent that is less than the doseor amount of said agent in free form to have a desired effect (e.g., adesired therapeutic effect).

In some embodiments, the agent is incorporated into a particle at a dosethat is less than the dose or amount of said agent in free form to havea desired effect (e.g., a desired therapeutic effect), e.g., thestandard of care dose for the intended use of the free agent. In oneembodiment, the agent are incorporated into the particles at a dose oramount of the agent that is less than the standard of care dose of theagent for a desired therapy (e.g., a dose that is less than about 0.01,about 0.02, about 0.03, about 0.04, about 0.05, about 0.06, about 0.07,about 0.08, about 0.09, about 0.1, about 0.2, about 0.3, about 0.4,about 0.5, about 0.6, about 0.7, about 0.8, about 0.9, or about 0.95that of the standard of care dose of the agent).

In some embodiments, the agent is incorporated into a particle at a doseequivalent to the dose or amount of said agent in free form to have adesired effect (e.g., a desired therapeutic effect), e.g., the standardof care dose for the intended use of the free agent. In theseembodiments, the particle produces a greater therapeutic effect and/or aless adverse effect than the free agent. In certain embodiments, theparticle increases the amount of the agent delivered to a tissue or cellin need thereof and reduces the amount of the agent exposed to anon-target tissue or cell, as compared to the free agent.

In some embodiments, the agent is incorporated into a particle at a dosehigher than the dose or amount of said agent in free form to have adesired effect (e.g., a desired therapeutic effect), e.g., the standardof care dose for the intended use of the free agent. In someembodiments, the agent is incorporated into a particle at a dose higherthan the dose or amount of said agent in free form that would produce anadverse effect by systemic administration (e.g., a reduction in bloodpressure). In some embodiments, since the particle described hereinreleases the agent at a target site based on pH microenvironment, othernon-target sites (e.g., blood vessels) with different pH would be lesslikely to be exposed to the agent.

In another aspect, provided are kits including a first containercomprising a polymer or composition described herein and instructionsfor use. The kits may further comprise a container (e.g., a vial,ampule, bottle, syringe, and/or dispenser package, or other suitablecontainer). In some embodiments, provided kits may optionally furtherinclude a second container comprising an excipient for dilution orsuspension of a composition or polymer described herein. In someembodiments, the composition described herein provided in the firstcontainer and the second container are combined to form one unit dosageform.

In certain embodiments, the kits are useful for delivering an agent(e.g., to a subject or cell). In certain embodiments, the kits areuseful for treating a disease (e.g., proliferative disease,hematological disease, neurological disease, painful condition,psychiatric disorder, or metabolic disorder) in a subject in needthereof. In certain embodiments, the kits are useful for preventing adisease (e.g., proliferative disease, hematological disease,neurological disease, painful condition, psychiatric disorder, ormetabolic disorder) in a subject in need thereof. In certainembodiments, the kits are useful for reducing the risk of developing adisease (e.g., proliferative disease, hematological disease,neurological disease, painful condition, psychiatric disorder, ormetabolic disorder) in a subject in need thereof. In certainembodiments, the kits are useful for inhibiting the activity (e.g.,aberrant activity, such as increased activity) of a protein kinase in asubject or cell.

In certain embodiments, a kit described herein further includesinstructions for using the kit. A kit described herein may also includeinformation as required by a regulatory agency such as the U.S. Food andDrug Administration (FDA). In certain embodiments, the informationincluded in the kits is prescribing information. In certain embodiments,the kits and instructions provide for delivering an agent. In certainembodiments, the kits and instructions provide for treating a disease(e.g., proliferative disease, hematological disease, neurologicaldisease, painful condition, psychiatric disorder, or metabolic disorder)in a subject in need thereof. In certain embodiments, the kits andinstructions provide for preventing a disease (e.g., proliferativedisease, hematological disease, neurological disease, painful condition,psychiatric disorder, or metabolic disorder) in a subject in needthereof. In certain embodiments, the kits and instructions provide forreducing the risk of developing a disease (e.g., proliferative disease,hematological disease, neurological disease, painful condition,psychiatric disorder, or metabolic disorder) in a subject in needthereof. In certain embodiments, the kits and instructions provide forinhibiting the activity (e.g., aberrant activity, such as increasedactivity) of a protein kinase in a subject or cell. A kit describedherein may include one or more additional pharmaceutical agentsdescribed herein as a separate composition.

Methods of Treatment and Uses

The present disclosure also provides methods of using the compositionsdescribed herein, or a pharmaceutical composition thereof, fordelivering an agent. The present disclosure also provides methods ofusing the polymers described herein, or a pharmaceutical compositionthereof, for the treatment, prevention, or diagnosis of a disease orcondition.

In certain embodiments, the methods described herein include treating adisease, disorder, or condition from which a subject suffers, comprisingadministering to a subject in need thereof an effective amount of acomposition described herein. In certain embodiments, the methodsdescribed herein include implanting in a subject an effective amount ofthe composition described herein. In certain embodiments, the methodsdescribed herein comprise treating a disease or condition in a subjectin need thereof by administering to or implanting in the subject atherapeutically effective amount of a composition. In certainembodiments, the methods described herein comprise preventing a diseaseor condition in a subject in need thereof by administering to orimplanting in the subject a prophylactically effective amount of acomposition. In certain embodiments, the methods described hereincomprise diagnosing a disease or condition in a subject in need thereofby administering to or implanting in the subject a diagnosticallyeffective amount of a composition.

In certain embodiments, the disease or condition is a genetic disease,proliferative disease, hematological disease, neurological disease,painful condition, psychiatric disorder, metabolic disorder, long-termmedical condition, cancer (e.g. lung cancer, large bowel cancer,pancreas cancer, biliary tract cancer, or endometrial cancer), neoplasm,angiogenesis, inflammatory disease, autoinflammatory disease, liverdisease, lung disease, spleen disease, familial amyloid neuropathy,cardiovascular disease, viral infection, fibrotic condition, orautoimmune disease.

In some embodiments, the compositions are useful in treating lungcancer, head-and-neck cancer, esophagus cancer, stomach cancer, breastcancer, pancreas cancer, liver cancer, kidney cancer, prostate caner,glioblastomas, metastatic melanomas, peritoneal or pleuralmesotheliomas.

In some embodiments, the proliferative disease is a benign neoplasm. Alltypes of benign neoplasms disclosed herein or known in the art arecontemplated as being within the scope of the disclosure. In someembodiments, the proliferative disease is associated with angiogenesis.All types of angiogenesis disclosed herein or known in the art arecontemplated as being within the scope of the disclosure. In certainembodiments, the proliferative disease is an inflammatory disease. Alltypes of inflammatory diseases disclosed herein or known in the art arecontemplated as being within the scope of the disclosure. In certainembodiments, the inflammatory disease is rheumatoid arthritis. In someembodiments, the proliferative disease is an autoinflammatory disease.All types of autoinflammatory diseases disclosed herein or known in theart are contemplated as being within the scope of the disclosure. Insome embodiments, the proliferative disease is an autoimmune disease.All types of autoimmune diseases disclosed herein or known in the artare contemplated as being within the scope of the disclosure.

In certain embodiments, the disease is a cardiovascular disease. Incertain embodiments, the disease is atherogenesis or atherosclerosis. Incertain embodiments, the disease is arterial stent occlusion, heartfailure (e.g., congestive heart failure), a coronary arterial disease,myocarditis, pericarditis, a cardiac valvular disease, stenosis,restenosis, in-stent-stenosis, angina pectoris, myocardial infarction,acute coronary syndromes, coronary artery bypass grafting, acardio-pulmonary bypass procedure, endotoxemia, ischemia-reperfusioninjury, cerebrovascular ischemia (stroke), renal reperfusion injury,embolism (e.g., pulmonary, renal, hepatic, gastrointestinal, orperipheral limb embolism), or myocardial ischemia.

In certain embodiments, the disease is a viral infection. In certainembodiments, the disease is an infection caused by DNA virus. In certainembodiments, the disease is an infection caused by a dsDNA virus. Incertain embodiments, the disease is an infection caused by an ssDNAvirus. In certain embodiments, the disease is an infection caused by anRNA virus. In certain embodiments, the disease is an infection caused bya dsRNA virus. In certain embodiments, the disease is an infectioncaused by a (+)ssRNA virus. In certain embodiments, the disease is aninfection caused by a (−)ssRNA virus. In certain embodiments, thedisease is an infection caused by a reverse transcribing (RT) virus. Incertain embodiments, the disease is an infection caused by an ssRNA-RTvirus. In certain embodiments, the disease is an infection caused by adsDNA-RT virus. In certain embodiments, the disease is an infectioncaused by human immunodeficiency virus (HIV). In certain embodiments,the disease is an infection caused by acquired immunodeficiency syndrome(AIDS). In certain embodiments, the disease is an infection caused byhuman papillomavirus (HPV). In certain embodiments, the disease is aninfection caused by hepatitis C virus (HCV). In certain embodiments, thedisease is an infection caused by a herpes virus (e.g., herpes simplexvirus (HSV)). In certain embodiments, the disease is an infection causedby Ebola virus. In certain embodiments, the disease is an infectioncaused by severe acute respiratory syndrome (SARS). In certainembodiments, the disease is an infection caused by influenza virus. Incertain embodiments, the disease is an infection caused by an influenzavirus. In certain embodiments, the disease is an infection caused by aninfluenza A virus. In certain embodiments, the disease is human flu(e.g., human flu caused by HIN1, H2N2, H3N2, H5N1, H7N7, H1N2, H9N2,H7N2, H7N3, or H10N7 virus). In certain embodiments, the disease is birdflu (e.g., bird flu caused by H5N1 or H7N9 virus). In certainembodiments, the disease is swine influenza (e.g., swine influenzacaused by HIN1, H1N2, H2N1, H3N1, H3N2, H2N3, or influenza C virus). Incertain embodiments, the disease is equine influenza (e.g., equineinfluenza caused by H7N7 or H3N8 virus). In certain embodiments, thedisease is canine influenza (e.g., canine influenza caused by H3N8virus). In certain embodiments, the disease is an infection caused by aninfluenza B virus. In certain embodiments, the disease is an infectioncaused by an influenza C virus. In certain embodiments, the disease isDengue fever, Dengue hemorrhagic fever (DHF), Dengue shock syndrome(DSS), hepatitis A, hepatitis B, hepatitis D, hepatitis E, hepatitis F,infection caused by Coxsackie A virus, infection caused by Coxsackie Bvirus, fulminant viral hepatitis, viral myocarditis, infection caused byparainfluenza virus, infection caused by an RS virus (RSV) (e.g., RSVbronchiolitis, RSV pneumonia, especially an infant and childhoodinfection caused by RSV and RSV pneumonia in the patients withcardiopulmonary disorders), infection caused by measles virus, infectioncaused by vesicular stomatitis virus, infection caused by rabies virus,Japanese encephalitis, infection caused by Junin virus, infection causedby human cytomegalovirus, infection caused by varicellovirus, infectioncaused by cytomegalovirus, infection caused by muromegalovirus,infection caused by proboscivirus, infection caused by roseolovirus,infection caused by lymphocryptovirus, infection caused by macavirus,infection caused by percavirus, infection caused by rhadinovirus,infection caused by poliovirus, infection caused by Marburg virus,infection caused by Lassa fever virus, Venezuelan equine encephalitis,infection caused by Rift Valley Fever virus, infection caused by Koreanhemorrhagic fever virus, infection caused by Crimean-Congo hemorrhagicfever virus, encephalitis, Saint Louise encephalitis, Kyasanur Forestdisease, Murray Valley encephalitis, tick-borne encephalitis, West Nileencephalitis, yellow fever, infection caused by adenovirus, infectioncaused by poxvirus, or a viral infection in subjects with immunedisorders.

In certain embodiments, the disease is a fibrotic condition. In certainembodiments, the disease is selected from the group consisting of renalfibrosis, post-operative stricture, keloid formation, hepatic cirrhosis,biliary cirrhosis, and cardiac fibrosis. In certain embodiments, thedisease is scleroderma. In certain embodiments, the disease isidiopathic pulmonary fibrosis.

In certain embodiments, the methods described herein include contactinga cell with an effective amount of a composition thereof. In certainembodiments, the cell is in vitro. In certain embodiments, the cell isin vivo.

EXAMPLES

In order that the present disclosure may be more fully understood, thefollowing examples are set forth. The synthetic and biological examplesdescribed in this application are offered to illustrate the compounds,pharmaceutical compositions, and methods provided herein and are not tobe construed in any way as limiting their scope.

In some examples, polylactone diacrylates with different numbers oflactone units have been synthesized according to FIG. 4 and used in thesynthesis of novel polylactone-based PBAEs with the same procedure shownin FIG. 1. These novel carriers have further been formulated witholigonucleotides and polynucleotides to form nanoparticles, which aretransfected into cells.

The present disclosure provides formulations of the polymers describedherein with mRNA to form nanoparticles. Such nanoparticles can be usedto transfect the mRNA into cells, including, e.g., HeLa cells. Thepotency of these novel nanoparticles has been evaluated and iscomparable to the potency of one of the current top performing PBAEspolymers (DD90-C12-122).^(9f) The polylactone-based PBAEs with 5 unitsof ϵ-caprolactone (caprolactone or CL) shows a higher transfectionefficiency than DD90-C12-122. In particular, the PBAE C1 is five timesmore effective and 35% less cytotoxic than DD90-C12-122. In addition, C1does not release compounds that may induce side effects that aredifficult to evaluate with a cellular assay, such as those related to asystematic toxicity and genotoxicity.

Experimental Section Materials

All chemicals were used as received except when specifically noted. Allthe solvents used were of analytical-grade purity and were purchasedfrom Sigma Aldrich. ϵ-caprolactone (CL), acryloyl chloride,triethylamine, 2-hydroxyethyl acrylate (HEA), 4-methoxyphenol, tin(II)2-ethylhexanoate, bisphenol A glycerolate (DD), ethylene glycoldiacrylate (A), dodecyl amine (C12), 4-(2-aminomethyl) morpholine (4),(+/−)-3amino-1,2-propanediol (1), N—N′-dimethyl ethylene diamine (3)were obtained from Sigma Aldrich (St. Louis, Mo., USA). 14:0 PEG2000 PE(PEG-lipid) was purchased from Avanti Polar Lipids (Alabaster, Ala.,USA). 5-amino-1-pentaol (2) and heparin sodium salt were obtained fromALFA Aesar (Haverhill, Mass., USA).

Firefly luciferase (FLuc) mRNA was generously provided by ShirePharmaceuticals (Lexington, Mass.). FLuc mRNA contained a 5′ cap (Cap1),a 5′ UTR consisting of a partial sequence of the cytomegalovirus (CMV)immediate early 1 (IE1) gene, a coding region as described below, a 3′UTR consisting of a partial sequence of the human growth hormone (hGH)gene, and a 3′ polyA tail.

Firefly luciferase (FLuc) mRNA sequence:

(SEQ ID NO: 1) AUGGAAGAUGCCAAAAACAUUAAGAAGGGCCCAGCGCCAUUCUACCCACUCGAAGACGGGACCGCCGGCGAGCAGCUGCACAAAGCCAUGAAGCGCUACGCCCUGGUGCCCGGCACCAUCGCCUUUACCGACGCACAUAUCGAGGUGGACAUUACCUACGCCGAGUACUUCGAGAUGAGCGUUCGGCUGGCAGAAGCUAUGAAGCGCUAUGGGCUGAAUACAAACCAUCGGAUCGUGGUGUGCAGCGAGAAUAGCUUGCAGUUCUUCAUGCCCGUGUUGGGUGCCCUGUUCAUCGGUGUGGCUGUGGCCCCAGCUAACGACAUCUACAACGAGCGCGAGCUGCUGAACAGCAUGGGCAUCAGCCAGCCCACCGUCGUAUUCGUGAGCAAGAAAGGGCUGCAAAAGAUCCUCAACGUGCAAAAGAAGCUACCGAUCAUACAAAAGAUCAUCAUCAUGGAUAGCAAGACCGACUACCAGGGCUUCCAAAGCAUGUACACCUUCGUGACUUCCCAUUUGCCACCCGGCUUCAACGAGUACGACUUCGUGCCCGAGAGCUUCGACCGGGACAAAACCAUCGCCCUGAUCAUGAACAGUAGUGGCAGUACCGGAUUGCCCAAGGGCGUAGCCCUACCGCACCGCACCGCUUGUGUCCGAUUCAGUCAUGCCCGCGACCCCAUCUUCGGCAACCAGAUCAUCCCCGACACCGCUAUCCUCAGCGUGGUGCCAUUUCACCACGGCUUCGGCAUGUUCACCACGCUGGGCUACUUGAUCUGCGGCUUUCGGGUCGUGCUCAUGUACCGCUUCGAGGAGGAGCUAUUCUUGCGCAGCUUGCAAGACUAUAAGAUUCAAUCUGCCCUGCUGGUGCCCACACUAUUUAGCUUCUUCGCUAAGAGCACUCUCAUCGACAAGUACGACCUAAGCAACUUGCACGAGAUCGCCAGCGGCGGGGCGCCGCUCAGCAAGGAGGUAGGUGAGGCCGUGGCCAAACGCUUCCACCUACCAGGCAUCCGCCAGGGCUACGGCCUGACAGAAACAACCAGCGCCAUUCUGAUCACCCCCGAAGGGGACGACAAGCCUGGCGCAGUAGGCAAGGUGGUGCCCUUCUUCGAGGCUAAGGUGGUGGACUUGGACACCGGUAAGACACUGGGUGUGAACCAGCGCGGCGAGCUGUGCGUCCGUGGCCCCAUGAUCAUGAGCGGCUACGUUAACAACCCCGAGGCUACAAACGCUCUCAUCGACAAGGACGGCUGGCUGCACAGCGGCGACAUCGCCUACUGGGACGAGGACGAGCACUUCUUCAUCGUGGACCGGCUGAAGAGCCUGAUCAAAUACAAGGGCUACCAGGUAGCCCCAGCCGAACUGGAGAGCAUCCUGCUGCAACACCCCAACAUCUUCGACGCCGGGGUCGCCGGCCUGCCCGACGACGAUGCCGGCGAGCUGCCCGCCGCAGUCGUCGUGCUGGAACACGGUAAAACCAUGACCGAGAAGGAGAUCGUGGACUAUGUGGCCAGCCAGGUUACAACCGCCAAGAAGCUGCGCGGUGGUGUUGUGUUCGUGGACGAGGUGCCUAAAGGACUGACCGGCAAGUUGGACGCCCGCAAGAUCCGCGAGAUUCUCAUUAAGGCCAAGAAGGGCGGCAAGAUCGCCGUGUAA

General Methods and Instruments 1) Synthesis of the Polylactone-basedDiacrylates

Polylactone-based diacrylates were synthesized in a two-step procedure:(i) ring opening polymerization of CL with HEA as initiator and tin(II)2-ethylhexanoate as catalyst and (ii) esterification with acryloylchloride (see FIG. 4). In the first step, the ring openingpolymerization was carried out in bulk with a CL to HEA ratio and HEA totin(II) 2-ethylhexanoate ratio equal to 3, 5 and 7 and 1/200,respectively. As an example for q=3, 1 g of CL, 17 mg of Na₂SO₄ and 1 mgof 4-methoxyphenol were weighted in a septa-sealed flask and heated to130° C. in a constant temperature oil bath under stirring. 1 g of HEAwere mixed with 17 mg of tin(II) 2-ethylhexanoate in a different vialand injected into the pre-heated CL containing flask. The polymerizationwas allowed to proceed for 3 hours. An aliquot was taken to perform ¹HNMR (in CDCl₃) and GPC (in THF). In the second step, the esterificationwas carried out in dichloromethane with 2 equivalent of triethylamineand acryloyl chloride. As an example for q=3, 3.95 g of the previousmacro-monomer and 2.4 ml of triethylamine were dissolved in 50 ml of DCMand poured in a septum sealed round-bottom flask. 1.4 ml of acryloylchloride were added dropwise into the reactor under inert atmosphere at0° C. and under stirring. The reaction was left to equilibrate to roomtemperature and then stopped after 24 h. The final mixture was filteredand washed several times with water and brine. The mixture was driedover Na_(s)SO₄ and the solvent eliminated via rotavapor. The finaldiacrylate was dried under vacuum and characterized via NMR and GPC. ThePCL-based custom diacrylates were characterized via ¹H NMR (400 MHz,CDCl₃) on a Bruker spectrometer.

Polylactone-based diacrylates and PBAE polymers were dissolved intetrahydrofuran (THF) at a concentration of 4 mg ml⁻¹, filtered over 0.2um PTFE syringe filter and eluted in Styragel columns at a 1 ml min⁻¹flow rate. The instrument is equipped with a Malvern Viscotek™ TDA 305triple detector. Molecular weights and polydispersities were relative tolinear polystyrene standards.

2) PBAE Synthesis

In certain embodiments, alkyl-side chain containing polymers weresynthesized as shown in FIG. 5. Briefly, the diacrylate monomer, the C12amine and the hydrophilic amine were dissolved in DMSO at aconcentration of 0.6 M and added in a 4 ml vials in such way that theirmolar ratio was 1.2:0.3:0.7. The hydrophobic C12 amine was heated to 60°C. before addition to the reaction medium in order to avoid crashingout. The reaction was purged with nitrogen for 2 minutes and heated to90° C. After 2 days, the end capping was added and the reactionre-started at 90° C. for an additional day. The final concentration wasadjusted to 100 mg ml⁻¹ by adding more DMSO and the samples were dividedinto aliquots and stored at −80° C.

3) Nanoparticle Formulation

Two different procedures were adopted to produce the nanoparticles(NPs). In the first case, 5 ul of the 100 mg ml⁻¹ polymer solution inDMSO were dissolved in 195 ul of 25 mM NaOAc and then mixed by pipettingwith 200 ul of a solution of 50 ng/ul of mRNA in 25 mM NaOAc buffer. Inthe second case, 5 ul of the 100 mg ml⁻¹ polymer solution in DMSO weredissolved in 45 ul of ethanol with different amount of PEG-lipid whenspecifically noted and mixed by pipetting with 50 ul of a solution of200 ng/ul of mRNA in 25 mM NaOAc buffer. The final mixture was finallydiluted with PBS to reach the mRNA final concentration of 5 ng/ul.

Nanoparticle (NP) size, PDI (polydispersity index), and (potential(i.e., zeta potential; electrokinetic potential) were obtained using aZetasizer (Malvern). For size measurement, NPs were diluted in PBS at a1/40 v/v ratio and an intensity average size was reported. For the ζpotential measurements, NPs were diluted in deionized water at a ratioof 1/40 v/v.

4) Transfection Tests

20000 HeLa cells in 100 ul of freshly prepared medium were seeded intoeach well of a 96-well white polystyrene tissue culture plate (Costar®)and left to grow for a day. In a typical experiment, for a mRNA dose of50 ng/well, 50 ul of the 5 ug/ul mRNA NP formulation were mixed with 450ul of freshly prepared medium pre-heated to 37° C. The conditionedmedium was removed by the wells and replaced with 100 ul of the dilutedmRNA NP formulation. The HeLa cells were left to incubate for anotherday. The MultiTox-Fluor Multiplex Cytotoxicity Assay (Promega) andcellular Bright-Glo kits (Promega) were carried out according to thespecifications of the producer in order to evaluate the cell viabilityand the cellular luminescence via a Tecan plate reader. Cellularluminescence was normalized to live cell count that, in turn, wasdetermined via a standard curve made using the viability assay.

5) In Vivo Experiments

20 ul of PBAE (100 ug/ul in DMSO) and different amount of PEG-lipid weredissolved in 180 ul of ethanol and then mixed by repeated pipetting tothe aqueous phase consisting of 40 ul of 1 mg/ml mRNA and 160 ul of 25mM NaOAc buffer. After 10 min, the NPs were dialyzed against 4 L of PBSat 4° C. in 20000 MWCO cassettes (Thermo Fisher). The mRNA concentrationof the final NPs were evaluated via a modified Quanti-iT RiboGreen RNAassay (Thermo Fisher) according to a previous work²⁴. In vivo jetPEI(Polyplus) was used according to manufacturer instructions at an N/P of8, the maximum recommended polymer:nucleic acid ratio.

NP formulations were injected via tail vein in female C57BL/6 mice(Charles River Laboratories, 18-22 g). 130 uL of 30 mg ml⁻¹ D-luciferin(PerfkinElmer) in PBS were injected intraperitoneally 24 hours after theadministration of the NP formulation for luciferase image experiments.Mice were sacrificed via CO₂ asphyxiation 10 min after D-luciferininjection. The luminescence of the pancreas, spleen, kidneys, liver,lungs, and heart was quantified via an IVIS imaging apparatus(PerkinElmer). 24 hours following intravenous nanoparticle injection inmice, blood samples were collected via the tail vein in serum collectiontubes (Sarstedt). Serum was collected from the blood, and ALT and ASTactivities were analyzed using AST and ALT colorimetric/fluorometricactivity assay kits (Sigma). Assays were performed according tomanufacturer instructions, and enzyme activities were normalized tothose of PBS-treated mice.

6) Nanoparticle Stability Test

After synthesizing NPs for the in vivo tests, the final concentrationwas adjusted to 50 ng/ul mRNA. 90 ul of NP solution was added to eachwell of a transparent 96 well plate. The absorbance of each plate wasmeasured initially at 660 nm. After the addition of 10 ul of fetalbovine serum, the plate was incubated at 37° C. and the absorbance wasmeasured at the same wavelength after 15, 30, 45, 60, 90 and 120minutes.

DISCUSSION

The polylactone-based diacrylates were synthesized via a novel two-stepprocedure that consists of (i) a ring opening polymerization ofcaprolactone (CL) with 2-hydroxyethylacrylate (HEA) as initiator andtin(II)-ethylhexanoate as catalyst and (ii) acylation with acryloylchloride (see FIG. 4).

The ROP is a so-called “living polymerization”, a technique that allowsfor control over the number of the units of caprolactone (q) that can beattached to the final diacrylate. In addition to PCL being inherentlymore biocompatible than other diacrylates, the tunability of q byvarying the reaction stoichiometry allows for control over thediacrylate's (and therefore the PBAE's) lipophilicity and molecularweight. In addition, the polymerization does not require any solvent orfurther purification steps due to the nearly complete conversion and theabsence of by-products²⁵. For this reason, the obtained macromonomer canbe directly used in the second step of the synthesis of the customdiacrylates, an esterification with acryloyl chloride. This latter stepwas used to attach the additional double bond required to produce thePBAE polymers via Michael step-growth polymerization. An ethylenglycoldiacrylate (A), which has a q value equal to 0, has been used inprevious PBAEs.^(9f) Three different PCL-based diacrylates weresynthesized with a low polydispersity (Ð<1.3) and theoretical q valuesvery close to the one evaluated via ¹H NMR (data in table of FIG. 8A andrepresentative ¹H NMR of a custom diacrylate in FIG. 8B).

In certain embodiments, PBAEs incorporating the custom diacrylates weresynthesized in a two-step reaction.^(9f) In the first step, as shown inFIG. 5, an alkyl amine (dodecylamine) and different hydrophilic amines(1, 2, 3, 4) were reacted with the custom diacrylates (A, B, C, D) at aratio equal to 1.2:0.7:0.3 (diacrylate:hydrophilic amine:dodecyl amine)to produce a library of acrylate-terminated PBAE polymers. The choice toincorporate a lipophilic amine in the PBAE is justified by the superiorstability and efficiency of the polymers to deliver DNA compared toclassic PBAE polymers.^(9f) The second step is carried out without anyintermediate purification and consists in another Michael addition withan excess of a PEGylated diamine in order to completely consume theremaining double bonds in the mixture. In fact, the end-capping of thePBAE polymers has shown to increase the transfection efficiency and toreduce their toxicity.^(26,27)

The PBAE polymers synthesized show a high Ð (see FIG. 9) as expectedfrom a step-growth polymerization, but in addition they show a linearbehavior of M_(w) within the adoption of the same hydrophilic amine in away similar to the custom diacrylate (FIG. 10A).

In fact, the M_(w) obtained via GPC is a linear function of the numberof CL units attached with the intercept equal to the M_(w) of thediacrylate A due to the control given by the ring openingpolymerization. Thus, despite the fact that Michael additionpolymerization is known to require high conversion and the completeabsence of impurities in the mixture, this novel strategy allowed us toimprove the control over the M_(w) and the lipophilicity of the finalmaterial. However, it is important to note that as long as the number ofamines per each polymer chain is theoretically the same for apre-assigned stoichiometry,²⁸ the increase of the M_(w) results in areduction by weight of the overall ionizable charges (i.e. chargedensity).

The lipophilicity of the polymer also plays an important role in theproduction of the NPs since it can affect its solubility in the mediumthat is used for the ionic complexation of the oligonucleotides. Forthis reason, two different procedures were adopted in order to evaluatethe effect of the formulation method on the NPs size and, in turn, ontheir transfection efficiency in vitro. In the first case, so calleddirect mixing, the polymer solution (polymer in DMSO) was directlydissolved in 25 mM NaOAc and then mixed with the mRNA. However, not allthe polymers are freely soluble in these aqueous phase and smallnanoparticles were produced. The addition of the oligonucleotide led toa partial coagulation of these primary NPs into larger nanoaggregrates,as visible in FIG. 11. In the second case, the polymer solution wasdissolved in ethanol before mixing with mRNA. In this case, referred toas pre-mixing protocol, the NPs were formed during the mixing of the twophases resulting into a higher size compared to the ones obtained withthe previous method (see FIG. 11).

However, as shown in FIG. 10B, all the NPs generated with the pre-mixingprotocol show a higher transfection efficiency compared to the onesobtained via direct mixing. This effect is more enhanced where thelipophilicity of the polymers is higher, and underscores the importanceof formulation conditions, in addition to material synthesis, whenconducting material screens. Thus, in order to find the best performingmaterials in the screening of the PBAE library, the pre-mixing procedurewas applied to all the polymers, which were then used to transfect HeLacells (see FIG. 10C). A dependence on the lipophilicity of the polymeron the transfection efficiency can be seen in all the testedformulations. In particular, the less lipophilic PBAEs that are basedonly on the A diacrylate show little efficacy in mRNA delivery while theinitial increase in the number of the CL units (q) leads to a highertransfection. However, as mentioned previously, the beneficial impact ofthe increased lipophilicity is counterbalanced by the reduction in thedensity of ionizable amines that are necessary to correctly condense themRNA. When this reduction is too high, it affects the transfection, asclearly visible in the polymers made with the D. In addition, as visiblein the cell viability test in FIG. 10D, almost all the tested polymersare non-toxic despite the lack of a wash step following transfection.

The high transfection efficacy of most of these polymers can also beascribed to their ability to form relative small and mono-dispersenanoparticles in PBS, as visible in FIG. 12.

However, the stability of the NPs in biologically relevant fluid is amore crucial property for applications in which a systemic delivery ofthe mRNA is required. For this reason, the top performing polymers wereco-formulated with 7 wt. % of a polyethylene glycol-modified lipid(PEG-lipid) and the stability of the resulting NPs were tested in amixture of 10% fetal bovine serum (FBS) according to a previousprotocol.²⁹ Briefly, the NPs were incubated at 37° C. and the opticalclarity of the solution was evaluated at different time points (see FIG.13A). Among the C derived polymers, the C1 showed the best stability andwas therefore optimized in order to find the minimum amount of PEG-lipidthat led to no change in the absorbance once incubated with the FBS (seeFIG. 13B).

The increase in PEG-lipid led to a higher stability, but at the sametime it reduced the transfection efficacy of the NP formulation due tothe charge shielding effect of the PEG tails, as already reported inliterature²⁹ and also demonstrated in FIGS. 14A and 14B.

However, as serum stability is critical for effective systemic deliveryof PBAE polymers,²⁹ C1 was formulated with 15 wt % PEG-lipid for in vivotesting with an mRNA coding for firefly luciferase. The stable C1 wasinjected in mice at three different doses (0.5, 0.25 and 0.125 mg/kgmRNA) and their efficacy and toxicity compared with PEI. Interestingly,this PCL-based polymer has been found to work selectively into thespleen (see FIG. 13C) in contrast with other members of the PBAEfamily²⁹ that also show activity into the lungs, as well as lipidnanoparticles that are typically effective in the liver.^(30,31) Inaddition, even at the highest tested concentration, there is nosignificant difference in body weight (see FIG. 15) or in levels of theliver enzymes ALT and AST compared to PBS (p>0.05) indicating littlesystemic toxicity caused by the polymer (see FIG. 13D). The efficacy ofthe C1 is also higher in the spleen, as well as more specificallytargeted, compared to PEI at all the tested concentrations, and alsopossesses a much wider therapeutic window overall (see FIGS. 13E and13F).

Given its spleen-targeting capability, this novel class of PBAE polymersmay be used as novel biodegradable and biocompatible carriers for theintravenous delivery of antigen-encoding RNA for cancer immunotherapyand vaccination, as recently shown with a non-biodegradable lipidformulation.³²

A new synthesis for the production of well controlled poly-caprolactonebased diacrylates has been developed. The versatility of this method hasbeen demonstrated by varying the number of caprolactone units attachedto each diacrylate and is to some extent reflected in thecharacteristics of the PBAE polymers synthesized from them viastep-growth polymerization. The ability of the novel PCL-based PBAEmaterials to deliver mRNA has been shown to depend on the physiochemicalcharacteristics of the material, such as lipophilicity, as well as theformulation method used to complex the polymer with the oligonucleotide.This latter variable represents a previously unstudied aspect of PBAElibrary screens that could play an important role in identifying truetop candidates for nucleic acid delivery. The most stable polymer, theC1, was injected via IV in mice and showed a transfection efficacyseveral times higher than the PEI and selectively to the spleen, openingthe possibility to use a completely biodegradable and biocompatiblecarrier in the intravenously delivery of antigen-encoding mRNA forcancer immunotherapy and vaccination. The adoption of a controlledliving polymerization for the synthesis of more biocompatible and morebiodegradable custom diacrylates from other lactones will lead to thesynthesis of more versatile PBAE polymers with more controlledproperties, higher efficacy and lower toxicity for a variety ofapplications.

EQUIVALENTS AND SCOPE

In the claims articles such as “a,” “an,” and “the” may mean one or morethan one unless indicated to the contrary or otherwise evident from thecontext. Claims or descriptions that include “or” between one or moremembers of a group are considered satisfied if one, more than one, orall of the group members are present in, employed in, or otherwiserelevant to a given product or process unless indicated to the contraryor otherwise evident from the context. The invention includesembodiments in which exactly one member of the group is present in,employed in, or otherwise relevant to a given product or process. Theinvention includes embodiments in which more than one, or all of thegroup members are present in, employed in, or otherwise relevant to agiven product or process.

Furthermore, the invention encompasses all variations, combinations, andpermutations in which one or more limitations, elements, clauses, anddescriptive terms from one or more of the listed claims is introducedinto another claim. For example, any claim that is dependent on anotherclaim can be modified to include one or more limitations found in anyother claim that is dependent on the same base claim. Where elements arepresented as lists, e.g., in Markush group format, each subgroup of theelements is also disclosed, and any element(s) can be removed from thegroup. It should it be understood that, in general, where the invention,or aspects of the invention, is/are referred to as comprising particularelements and/or features, certain embodiments of the invention oraspects of the invention consist, or consist essentially of, suchelements and/or features. For purposes of simplicity, those embodimentshave not been specifically set forth in haec verba herein. It is alsonoted that the terms “comprising” and “containing” are intended to beopen and permits the inclusion of additional elements or steps. Whereranges are given, endpoints are included. Furthermore, unless otherwiseindicated or otherwise evident from the context and understanding of oneof ordinary skill in the art, values that are expressed as ranges canassume any specific value or sub-range within the stated ranges indifferent embodiments of the invention, to the tenth of the unit of thelower limit of the range, unless the context clearly dictates otherwise.

This application refers to various issued patents, published patentapplications, journal articles, and other publications, all of which areincorporated herein by reference. If there is a conflict between any ofthe incorporated references and the instant specification, thespecification shall control. In addition, any particular embodiment ofthe present invention that falls within the prior art may be explicitlyexcluded from any one or more of the claims. Because such embodimentsare deemed to be known to one of ordinary skill in the art, they may beexcluded even if the exclusion is not set forth explicitly herein. Anyparticular embodiment of the invention can be excluded from any claim,for any reason, whether or not related to the existence of prior art.

Those skilled in the art will recognize or be able to ascertain using nomore than routine experimentation many equivalents to the specificembodiments described herein. The scope of the present embodimentsdescribed herein is not intended to be limited to the above Description,but rather is as set forth in the appended claims. Those of ordinaryskill in the art will appreciate that various changes and modificationsto this description may be made without departing from the spirit orscope of the present invention, as defined in the following claims.

REFERENCES

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1. A polymer of Formula (I):

or a pharmaceutically acceptable salt thereof, wherein:

is a single bond or a double bond A is hydrogen or —XR₃; each Bindependently is a diradical of the formula:

each D independently is:

E is hydrogen or —XR₄; each R, R′, and R″ independently is optionallysubstituted, cyclic or acyclic aliphatic; optionally substituted, cyclicor acyclic heteroaliphatic; or a combination thereof; each R_(a)independently is hydrogen or optionally substituted aliphatic; R₁ and R₂independently are selected from hydrogen, optionally substituted, cyclicor acyclic aliphatic; optionally substituted, cyclic or acyclicheteroaliphatic; optionally substituted aryl; optionally substitutedheteroaryl; and a nitrogen protecting group; or R₁ and R₂ are combinedto form a ring; R₃ and R₄ independently are selected from hydrogen;optionally substituted, cyclic or acyclic aliphatic; optionallysubstituted, cyclic or acyclic heteroaliphatic; optionally substitutedaryl; optionally substituted heteroaryl; and a protecting group; X is O,S, NH, or NR_(X), wherein R_(X) is optionally substituted, aliphatic;optionally substituted carbocyclyl; optionally substitutedheteroaliphatic; optionally substituted heterocyclyl; optionallysubstituted aryl; or optionally substituted heteroaryl; X′ is O orNR_(y), wherein R_(y) is hydrogen; optionally substituted, aliphatic;optionally substituted carbocyclyl; optionally substitutedheteroaliphatic; optionally substituted heterocyclyl; optionallysubstituted aryl; or optionally substituted heteroaryl; each mindependently is an integer between 1 and 100, inclusive; and n is aninteger between 1 and 10,000, inclusive. 2.-4. (canceled)
 5. The polymerof claim 1, having the structure of Formula (III) or Formula (V):

6.-35. (canceled)
 36. The polymer of claim 1, wherein B is of theformula:


37. (canceled)
 38. The polymer of claim 1, wherein m is an integerbetween 3 and 7, inclusive 39.-85. (canceled)
 86. The polymer of claim1, wherein the polymer is of the formula:

wherein: each D is

87.-93. (canceled)
 94. The polymer of claim 1, wherein the polymer has amolecular weight between 1-100 kDa. 95.-113. (canceled)
 114. A method ofpreparing a polymer of Formula (III), as defined in claim 5, or apharmaceutically acceptable salt thereof:

comprising reacting a polymer of Formula (V), as defined in claim 1, ora pharmaceutically acceptable salt thereof:

with one or more nucleophiles selected from R₃XH and R₄XH; to obtain thepolymer of Formula (III). 115.-117. (canceled)
 118. A compositioncomprising a polymer of claim 1 and an excipient. 119.-123. (canceled)124. The composition of claim 118, wherein the composition furthercomprises an agent.
 125. (canceled)
 126. The composition of claim 124,wherein the agent is a polynucleotide.
 127. The composition of claim126, wherein the polynucleotide is DNA.
 128. The composition of claim126, wherein the polynucleotide is RNA.
 129. The composition of claim128, wherein the RNA is dsRNA, siRNA, shRNA, miRNA, mRNA, or antisenseRNA.
 130. The composition of claim 126, wherein the polynucleotidecarries out RNA interference.
 131. (canceled)
 132. The composition ofclaim 118, wherein the composition is in the form of a particle.133.-134. (canceled)
 135. The composition of claim 132, wherein theparticle encapsulates an agent.
 136. A method of treating a disease,disorder, or condition from which a subject suffers, comprisingadministering to a subject in need thereof an effective amount of acomposition of claim
 118. 137. (canceled)
 138. A method of delivering apolynucleotide to a cell, comprising contacting the cell with acomposition of claim
 126. 139.-140. (canceled)
 141. The composition ofclaim 128, wherein the RNA is mRNA.
 142. The composition of claim 141,wherein the mRNA encodes an antigen.